##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544852_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1451807
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92257648571746	34.0	33.0	34.0	31.0	34.0
2	33.27686806855181	34.0	33.0	34.0	31.0	34.0
3	33.34386802102483	34.0	34.0	34.0	31.0	34.0
4	36.61839693568084	37.0	37.0	37.0	35.0	37.0
5	36.618367317418915	37.0	37.0	37.0	35.0	37.0
6	36.67879614852387	37.0	37.0	37.0	35.0	37.0
7	36.655535480955805	37.0	37.0	37.0	35.0	37.0
8	36.548064584342136	37.0	37.0	37.0	35.0	37.0
9	38.360973600485465	39.0	39.0	39.0	37.0	39.0
10	38.17384886558613	39.0	39.0	39.0	35.0	39.0
11	37.64575250016014	39.0	37.0	39.0	35.0	39.0
12	37.580998714016395	39.0	37.0	39.0	35.0	39.0
13	37.565353383748665	39.0	37.0	39.0	35.0	39.0
14	38.911119039927485	41.0	37.0	41.0	35.0	41.0
15	38.966293729125155	41.0	37.0	41.0	35.0	41.0
16	38.94338848070026	41.0	37.0	41.0	35.0	41.0
17	38.98325810524402	41.0	37.0	41.0	35.0	41.0
18	38.967660990751526	41.0	36.0	41.0	35.0	41.0
19	38.908224715819664	41.0	36.0	41.0	35.0	41.0
20	38.83317066249164	41.0	35.0	41.0	35.0	41.0
21	38.76665562295815	41.0	35.0	41.0	35.0	41.0
22	38.73649458915682	41.0	35.0	41.0	35.0	41.0
23	38.69091277284102	41.0	35.0	41.0	35.0	41.0
24	38.62876401615366	41.0	35.0	41.0	35.0	41.0
25	38.58280336160385	40.0	35.0	41.0	35.0	41.0
26	38.495420534547634	40.0	35.0	41.0	35.0	41.0
27	38.478870125299025	40.0	36.0	41.0	35.0	41.0
28	38.49986809541489	40.0	36.0	41.0	35.0	41.0
29	38.49390311522124	40.0	36.0	41.0	35.0	41.0
30	38.446537315221654	40.0	35.0	41.0	35.0	41.0
31	38.388797546781355	40.0	35.0	41.0	35.0	41.0
32	38.30817319381984	40.0	35.0	41.0	35.0	41.0
33	38.197574470986844	40.0	35.0	41.0	35.0	41.0
34	38.111963918069	40.0	35.0	41.0	35.0	41.0
35	38.02521822804271	40.0	35.0	41.0	35.0	41.0
36	37.9676623683451	40.0	35.0	41.0	35.0	41.0
37	37.9024822169889	40.0	35.0	41.0	35.0	41.0
38	37.82543271936284	40.0	35.0	41.0	35.0	41.0
39	37.695400283922034	40.0	35.0	41.0	35.0	41.0
40	37.58190103781012	40.0	35.0	41.0	34.0	41.0
41	37.52536184217324	39.0	35.0	41.0	34.0	41.0
42	37.47415048970008	39.0	35.0	41.0	34.0	41.0
43	37.45992752480185	39.0	35.0	41.0	34.0	41.0
44	37.419472422987354	39.0	35.0	41.0	34.0	41.0
45	37.37861230866086	39.0	35.0	41.0	34.0	41.0
46	37.30015697678824	39.0	35.0	41.0	34.0	41.0
47	37.202723915782194	39.0	35.0	41.0	34.0	41.0
48	37.154030115573214	38.0	35.0	41.0	34.0	41.0
49	37.082665946644426	38.0	35.0	41.0	34.0	41.0
50	36.990960230939784	38.0	35.0	41.0	34.0	41.0
51	36.88251537566632	38.0	35.0	41.0	34.0	41.0
52	36.60173769653955	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	4.0
14	9.0
15	17.0
16	26.0
17	60.0
18	94.0
19	195.0
20	337.0
21	585.0
22	851.0
23	1515.0
24	2525.0
25	4005.0
26	5812.0
27	6858.0
28	7314.0
29	8070.0
30	9370.0
31	11444.0
32	15089.0
33	22191.0
34	72772.0
35	243583.0
36	62115.0
37	98116.0
38	169672.0
39	702856.0
40	6316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.45809257015567	21.89781424114913	23.56718213922374	20.07691104947145
2	28.11027912112285	24.22222788566249	25.28848531519686	22.37900767801781
3	28.16600278136143	25.154169941321403	24.651761563348295	22.028065713968868
4	26.757964385073223	27.26078604111979	22.02379517387642	23.95745439993057
5	25.773329375047787	35.36613337723265	19.660671149815368	19.1998660979042
6	87.85582381129173	3.4474279294699643	6.29484497595066	2.4019032832876546
7	88.92235675954173	2.9831788936132693	4.825848063826665	3.268616283018335
8	79.36082413158223	6.005068166774233	8.880174844176947	5.753932857466592
9	51.30089605574295	22.56222762391971	12.987470097609394	13.149406222727952
10	39.280772168752456	23.877347333357672	21.798351984802387	15.043528513087484
11	39.399382975836325	18.612046918082086	23.402215308233117	18.586354797848475
12	24.07737392091373	29.50550589713371	25.91625470878705	20.50086547316551
13	17.472983667939335	31.43654769538926	27.919551290219708	23.1709173464517
14	16.376074781289798	26.362388389090285	36.99534442250244	20.266192407117476
15	19.626506829075765	18.871241149822257	40.79233672244313	20.709915298658842
16	19.953478664863855	21.49445484144931	28.040986164138896	30.511080329547934
17	21.87501506742976	23.9281116567147	27.877052528331937	26.319820747523604
18	26.073438136060783	27.77903674524231	24.521854488923115	21.62567062977379
19	31.378964283820093	22.870464187044146	23.80378383628127	21.946787692854492
20	25.96288625140945	26.652027438908892	24.96757489115289	22.417511418528772
21	22.943132248294713	24.392016294176845	24.770992287542352	27.89385916998609
22	22.980051756190733	27.36803170118342	24.009320798150167	25.642595744475678
23	23.45897216365536	21.930532088631615	32.660952867702115	21.94954288001091
24	22.473992755235372	22.01298106428747	29.088783839725252	26.424242340751903
25	21.637862332940948	29.603521680223338	25.498499456194935	23.260116530640783
26	20.116723503881715	29.385379737113816	27.19411051193444	23.30378624707003
27	22.80750816051996	26.270640656781513	30.791833900787086	20.13001728191144
28	20.066785736671612	25.896486240939737	29.022590468292275	25.014137554096376
29	22.739179519040754	26.683780970886627	27.44593461803119	23.131104892041435
30	26.263201651459184	24.70411011932027	24.465373152216515	24.567315077004036
31	27.736124705281075	26.98581836290912	24.488103446256975	20.789953485552832
32	28.134869166493893	26.55917763173755	24.17745609437067	21.128497107397884
33	20.918758485115447	26.396070552077518	26.991604255937602	25.69356670686944
34	24.697084392071396	23.918399621988325	27.513298944005644	23.871217041934635
35	26.28297011930649	24.814730883650512	24.957449578353046	23.94484941868995
36	23.674841077360835	28.522248480686486	24.73793004166532	23.064980400287364
37	25.144595665952846	25.590384947861526	26.460610811216647	22.80440857496899
38	23.089983723731873	25.14700645471471	25.606778311442223	26.156231510111194
39	27.27008479777271	23.24131237829822	26.576741949859727	22.91186087406935
40	18.45045519135808	22.77947413120339	31.80002576099991	26.970044916438617
41	21.480334507272662	26.32953278224998	25.096586529752233	27.093546180725127
42	23.62772737698606	23.453668428379252	27.23660927382221	25.68199492081248
43	22.759361264961527	27.051391817231906	26.924033290926413	23.265213626880158
44	22.595840907228027	27.26230139405582	24.67332090284728	25.468536795868875
45	22.28037197781799	26.31479253096314	25.267063735055693	26.13777175616318
46	23.439616973881513	24.896628821875083	27.25651550102734	24.40723870321606
47	23.732562248287824	20.87439997189709	31.235212393933903	24.157825385881182
48	21.273626590862285	22.172506400644163	28.136797797503387	28.417069210990164
49	20.306969177032485	25.828984155607458	29.230675978280864	24.633370689079197
50	19.770671997035418	25.752803230732457	27.816369531211794	26.660155241020327
51	22.084547050675468	23.02930072661173	28.04449902776333	26.841653194949465
52	23.927560619283415	20.63029038983832	29.594291803249327	25.847857187628936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	543.5
2	842.0
3	3027.5
4	5213.0
5	3495.5
6	1778.0
7	1852.5
8	1927.0
9	1973.5
10	2020.0
11	1964.0
12	1908.0
13	1794.5
14	1744.0
15	1807.0
16	1714.0
17	1621.0
18	1899.0
19	2177.0
20	2291.0
21	2405.0
22	2530.5
23	2656.0
24	3547.0
25	4438.0
26	5647.5
27	6857.0
28	9521.5
29	12186.0
30	12825.0
31	13464.0
32	15419.0
33	17374.0
34	22598.5
35	27823.0
36	29088.5
37	30354.0
38	33549.5
39	42129.0
40	47513.0
41	57503.0
42	67493.0
43	84949.0
44	102405.0
45	107565.5
46	112726.0
47	120574.5
48	128423.0
49	127874.5
50	127326.0
51	117958.0
52	108590.0
53	99125.5
54	89661.0
55	82727.0
56	75793.0
57	72270.5
58	68748.0
59	70888.5
60	73029.0
61	75775.5
62	78522.0
63	73847.5
64	56579.0
65	43985.0
66	35395.0
67	26805.0
68	22707.0
69	18609.0
70	15987.0
71	13365.0
72	11544.5
73	9724.0
74	8113.5
75	6503.0
76	5422.0
77	4341.0
78	3169.5
79	1998.0
80	1452.0
81	906.0
82	706.0
83	506.0
84	294.5
85	83.0
86	66.0
87	49.0
88	27.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1451807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.294465166279277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49475360255404	15.224425401613068
2	11.143169702361263	4.74575678137741
3	4.0235839298648095	2.5704020351432177
4	1.84890395073743	1.5748568309909738
5	1.1204323604691813	1.1929503935591526
6	0.6914434053641488	0.8834350505988233
7	0.5079586455203159	0.7571695378059937
8	0.41730880430077444	0.7109094237411598
9	0.35363639921081075	0.6777448186070737
>10	6.357086045983426	37.165625367311655
>50	1.8315509590403432	24.587211530487348
>100	0.19687426252831436	6.688530934115973
>500	0.008432834968185131	1.2552823127032793
>1k	0.004865097097029883	1.9656995819448713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	4002	0.27565647499977614	TruSeq Adapter, Index 14 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	3240	0.22317015966998366	Illumina PCR Primer Index 3 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3109	0.21414692173270963	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2780	0.19148550737115883	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2503	0.17240583631295345	TruSeq Adapter, Index 14 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1905	0.13121578832448114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.88796789104888E-5	0.0	0.0	0.22833613558827034	0.0
2	6.88796789104888E-5	0.0	0.0	0.8049968074268825	0.0
3	6.88796789104888E-5	0.0	0.0	1.3107802896666017	0.0
4	6.88796789104888E-5	0.0	0.0	1.63747660673905	0.0
5	6.88796789104888E-5	0.0	0.0	3.3007830930695334	0.0
6	6.88796789104888E-5	0.0	0.0	3.5759574103169363	0.0
7	6.88796789104888E-5	0.0	0.0	4.4164961320616305	0.0
8	6.88796789104888E-5	0.0	0.0	5.528283029355831	0.0
9	6.88796789104888E-5	0.0	0.0	6.281413438563114	0.0
10	6.88796789104888E-5	0.0	0.0	6.943967070003106	0.0
11	6.88796789104888E-5	0.0	0.0	7.541704923588328	0.0
12	6.88796789104888E-5	0.0	0.0	8.086543183770294	0.0
13	6.88796789104888E-5	0.0	0.0	8.353107541153886	0.0
14	6.88796789104888E-5	0.0	0.0	8.543284334625746	0.0
15	6.88796789104888E-5	0.0	0.0	9.135374054540307	0.0
16	6.88796789104888E-5	0.0	0.0	9.765347597855637	0.0
17	6.88796789104888E-5	0.0	0.0	10.517169293163622	0.0
18	6.88796789104888E-5	0.0	0.0	10.94381002433519	0.0
19	6.88796789104888E-5	0.0	0.0	11.399449100328074	0.0
20	6.88796789104888E-5	0.0	0.0	11.974594419230655	0.0
21	6.88796789104888E-5	0.0	0.0	12.514197823815424	0.0
22	6.88796789104888E-5	0.0	0.0	13.107251859234733	0.0
23	6.88796789104888E-5	0.0	0.0	13.541813753481007	0.0
24	6.88796789104888E-5	0.0	0.0	13.940007177262542	0.0
25	6.88796789104888E-5	0.0	0.0	14.306447069066342	0.0
26	6.88796789104888E-5	0.0	0.0	14.644921811232484	0.0
27	6.88796789104888E-5	0.0	0.0	14.99524385817123	0.0
28	6.88796789104888E-5	0.0	0.0	15.370706987912305	0.0
29	6.88796789104888E-5	0.0	0.0	15.790597510550645	0.0
30	6.88796789104888E-5	0.0	0.0	16.235766875349135	0.0
31	6.88796789104888E-5	0.0	0.0	16.67211964124708	0.0
32	6.88796789104888E-5	0.0	0.0	17.034013474242787	0.0
33	6.88796789104888E-5	0.0	0.0	17.414022662791954	0.0
34	6.88796789104888E-5	0.0	0.0	17.877858420575187	0.0
35	6.88796789104888E-5	0.0	0.0	18.28955226142318	0.0
36	6.88796789104888E-5	0.0	0.0	18.76461540686882	0.0
37	6.88796789104888E-5	0.0	0.0	19.160122523172845	0.0
38	6.88796789104888E-5	0.0	0.0	19.56410183998286	0.0
39	6.88796789104888E-5	0.0	0.0	19.986540910740892	0.0
40	6.88796789104888E-5	0.0	0.0	20.443213181917432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAAC	20	6.312005E-4	46.0	42
ATGTACG	20	6.312005E-4	46.0	6
ACAATCG	20	6.312005E-4	46.0	1
ACGCTAA	20	6.312005E-4	46.0	10
CGCAAAT	20	6.312005E-4	46.0	44
TGTACGA	20	6.312005E-4	46.0	38
GATTACG	20	6.312005E-4	46.0	10
GTACGAC	20	6.312005E-4	46.0	44
TAGTAGG	395	0.0	44.253166	1
CGGTCTA	100	0.0	43.7	30
ACGTAGG	190	0.0	43.57895	1
ACGGGTA	85	0.0	43.294117	4
CATGCGG	415	0.0	42.674698	1
ACTACGG	65	0.0	42.46154	1
CTAGCGG	425	0.0	42.211765	1
ATTGCGG	390	0.0	41.871796	1
ATCAACG	930	0.0	41.795696	14
CGTTTTT	1750	0.0	41.531425	1
CGCAAGC	925	0.0	41.524323	19
ATAGCGG	245	0.0	41.30612	1
>>END_MODULE