FastQCFastQC Report
Thu 26 May 2016
SRR1544851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544851_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1014698
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT51480.5073430715345847TruSeq Adapter, Index 14 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA46810.46131952561254674No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT42160.41549308267090307Illumina PCR Primer Index 3 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT31890.3142807022384985TruSeq Adapter, Index 14 (96% over 26bp)
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTTG23520.23179310494353986TruSeq Adapter, Index 14 (96% over 28bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21010.20705668090407198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.11855744270709119No Hit
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11420.1125458018050691TruSeq Adapter, Index 14 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11400.11234869882467494Illumina PCR Primer Index 3 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11390.11225014733447784Illumina PCR Primer Index 3 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT10300.10150803490299577TruSeq Adapter, Index 14 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA206.3111034E-446.018
CGACATA253.4168705E-546.030
TCGTATA301.8611863E-646.028
CGTAAGT253.4168705E-546.016
CGGTCTA750.046.030
CTAGCGG3750.043.546671
TCGGCGT5850.043.2478644
ACGTAGG750.042.933331
CATGCGG2750.042.654551
TTAATCG1800.042.16666819
CGTTGAT1800.042.16666824
GTCGGCG6050.041.818183
CGTAGGG3550.041.464792
CGTTTTT11800.041.3220331
TTACGGG2850.041.1578982
TAATCGT1850.041.02702720
CACGACG850.040.58823425
CACACGG1200.040.251
TTGAGGG10700.040.1962622
TAGGGTA3450.040.04
Produced by FastQC (version 0.11.5)