##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544851_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1014698
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90559949857002	34.0	33.0	34.0	31.0	34.0
2	33.276436930002816	34.0	33.0	34.0	31.0	34.0
3	33.376411503718344	34.0	34.0	34.0	31.0	34.0
4	36.59958233878454	37.0	37.0	37.0	35.0	37.0
5	36.61057477200113	37.0	37.0	37.0	35.0	37.0
6	36.516089516289576	37.0	37.0	37.0	35.0	37.0
7	36.407322178618664	37.0	37.0	37.0	35.0	37.0
8	36.43333977203069	37.0	37.0	37.0	35.0	37.0
9	38.28946149494727	39.0	39.0	39.0	37.0	39.0
10	38.17258336963313	39.0	39.0	39.0	35.0	39.0
11	37.708331937187225	39.0	37.0	39.0	35.0	39.0
12	37.68077102743871	39.0	37.0	39.0	35.0	39.0
13	37.671047937415864	39.0	37.0	39.0	35.0	39.0
14	38.98637032890574	41.0	38.0	41.0	35.0	41.0
15	39.039370334818834	41.0	38.0	41.0	35.0	41.0
16	39.027899926874795	41.0	38.0	41.0	35.0	41.0
17	39.0790560344063	41.0	38.0	41.0	35.0	41.0
18	39.075229280041945	41.0	38.0	41.0	35.0	41.0
19	39.03005524796541	41.0	37.0	41.0	35.0	41.0
20	38.97148314079657	41.0	37.0	41.0	35.0	41.0
21	38.90615730000453	41.0	37.0	41.0	35.0	41.0
22	38.8813893394882	41.0	36.0	41.0	35.0	41.0
23	38.84586448381686	41.0	36.0	41.0	35.0	41.0
24	38.76873217449921	41.0	36.0	41.0	35.0	41.0
25	38.707213377773485	41.0	35.0	41.0	35.0	41.0
26	38.61723192516394	40.0	35.0	41.0	35.0	41.0
27	38.61083593345015	40.0	36.0	41.0	35.0	41.0
28	38.66893696449584	40.0	36.0	41.0	35.0	41.0
29	38.7048688378217	41.0	36.0	41.0	35.0	41.0
30	38.673999554547265	41.0	36.0	41.0	35.0	41.0
31	38.65578132606943	41.0	35.0	41.0	35.0	41.0
32	38.60215059061908	41.0	36.0	41.0	35.0	41.0
33	38.50576624769143	40.0	35.0	41.0	35.0	41.0
34	38.44896609631634	40.0	35.0	41.0	35.0	41.0
35	38.33969121847092	40.0	35.0	41.0	35.0	41.0
36	38.310762414038464	40.0	35.0	41.0	35.0	41.0
37	38.24378583578562	40.0	35.0	41.0	35.0	41.0
38	38.16584540424836	40.0	35.0	41.0	35.0	41.0
39	38.095245087700974	40.0	35.0	41.0	35.0	41.0
40	37.99601950531094	40.0	35.0	41.0	35.0	41.0
41	37.97359115717189	40.0	35.0	41.0	35.0	41.0
42	37.90595625496453	40.0	35.0	41.0	35.0	41.0
43	37.81832426988129	40.0	35.0	41.0	35.0	41.0
44	37.67607307790101	40.0	35.0	41.0	35.0	41.0
45	37.64948388584584	39.0	35.0	41.0	35.0	41.0
46	37.572470823831324	39.0	35.0	41.0	35.0	41.0
47	37.47473928203269	39.0	35.0	41.0	35.0	41.0
48	37.40803766243749	39.0	35.0	41.0	35.0	41.0
49	37.34864659238512	39.0	35.0	41.0	35.0	41.0
50	37.26260917041326	39.0	35.0	41.0	35.0	41.0
51	37.16848756970054	38.0	35.0	41.0	34.0	41.0
52	36.71120372761157	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	4.0
14	6.0
15	16.0
16	26.0
17	39.0
18	91.0
19	148.0
20	249.0
21	353.0
22	611.0
23	902.0
24	1443.0
25	2271.0
26	3318.0
27	3801.0
28	4220.0
29	4430.0
30	5327.0
31	6747.0
32	9129.0
33	13426.0
34	45543.0
35	162874.0
36	41190.0
37	66824.0
38	119241.0
39	518711.0
40	3752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.32617389607549	21.385180615316084	22.250561250736673	22.03808423787176
2	27.720858817106176	24.386664800758453	25.37779713767052	22.514679244464855
3	28.666953122998173	24.896767314018557	24.5539066796229	21.882372883360368
4	26.56682086689833	26.740271489645195	22.381831835679186	24.31107580777729
5	25.47536311296563	34.721661026236376	19.786576892829196	20.016398967968797
6	85.76808074914901	3.479261809917828	7.426840301252195	3.3258171396809693
7	87.0588096162602	3.1005284330904366	5.647493145743857	4.193168804905499
8	76.08736786708951	6.190610408219983	10.523229571754355	7.198792152936144
9	49.81255506564515	21.900703460537027	13.859098963435427	14.427642510382398
10	37.619271941011014	23.29776938557088	23.7261727134576	15.3567859599605
11	37.43606472073464	18.472491322541288	23.976000741107207	20.115443215616864
12	23.305949159257235	28.263778976601905	27.123636786511852	21.306635077629007
13	17.220296088097147	30.480004888153918	28.654239980762746	23.64545904298619
14	16.129035437144847	26.2095717149339	36.0893586071915	21.572034240729753
15	19.03748701584117	18.85152035383927	40.408673319549266	21.7023193107703
16	19.1918186494898	22.521183642817864	27.922495166049405	30.364502541642935
17	20.924748053115312	24.256182627737513	28.087076154678535	26.731993164468644
18	25.04015973225531	27.80886529785217	25.135853229236677	22.01512174065584
19	29.909588862893195	22.655410772466293	24.28752200162019	23.147478363020326
20	24.896767314018557	27.15290657910038	25.38311891814116	22.5672071887399
21	22.27973249183501	24.197938697031038	25.1082588119815	28.41406999915246
22	22.222375524540304	27.36981840902416	24.057502823500194	26.350303242935336
23	22.442046796189604	21.984669330184943	32.9164933802964	22.656790493329048
24	22.772095736859637	22.337188010619908	28.372185615818697	26.518530636701758
25	21.213799573863355	29.25836061567087	25.650981868496835	23.87685794196894
26	19.609479864945037	28.785707668685657	27.152118167178806	24.452694299190497
27	23.433770442042853	25.582094376849074	30.523466095330825	20.460669085777248
28	20.145797074597567	25.316005353316946	28.76205531103836	25.77614226104713
29	23.063118287411623	26.40805441619083	26.86612174262687	23.662705553770678
30	25.75406672724298	24.433575310092266	24.73070805303647	25.081649909628283
31	27.3211339728668	26.03838777646157	24.611559301388198	22.028918949283433
32	27.706963056988386	25.9831989419512	24.6216115533883	21.688226447672115
33	20.27273139397141	25.444417945043746	27.344687779023907	26.938162881960935
34	25.35345491959184	22.723509852192475	27.962605622559618	23.960429605656067
35	25.78353362281191	23.78934421867393	26.20218035316912	24.224941805345036
36	24.15585721071688	27.28447281851349	24.18828065099172	24.371389319777904
37	25.283976119002894	24.751108211507265	27.3988910986323	22.566024570857536
38	22.858623945252678	24.1686689044425	25.51557212096604	27.45713502933878
39	27.727166112478784	22.11140654657839	26.68330872831128	23.47811861263154
40	18.50757565305145	21.7327717212412	32.21825607225007	27.541396553457286
41	21.293232074962205	25.811325143047487	25.239036639473028	27.65640614251728
42	23.116434643608248	22.173888191363343	28.010994404246386	26.698682760782027
43	23.499997043455295	25.756826168968498	26.878440678901505	23.8647361086747
44	22.76194493336934	25.63294694579077	25.255297635355547	26.349810485484348
45	21.987231668930065	24.79801872084108	25.88494310622471	27.329806504004146
46	23.842956229341144	23.229965960315287	27.671484520517435	25.255593289826134
47	22.729422941604298	19.97668271741937	32.396240063546	24.897654277430327
48	21.10450597123479	20.726955212289766	28.26121663785678	29.90732217861866
49	20.496837482679574	23.977774667930753	29.894411933402843	25.630975915986827
50	19.976485614438978	23.98398341181317	28.672472006449212	27.367058967298647
51	22.138705309362983	21.51260769214091	28.116937256208253	28.231749742287853
52	23.352268359649866	19.86039195898681	31.090235715454252	25.69710396590907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	295.0
2	459.0
3	1473.0
4	2487.0
5	1748.5
6	1010.0
7	1047.5
8	1085.0
9	1117.5
10	1150.0
11	1138.0
12	1126.0
13	1075.5
14	1012.0
15	999.0
16	1019.5
17	1040.0
18	1215.5
19	1391.0
20	1496.5
21	1602.0
22	1671.0
23	1740.0
24	2241.5
25	2743.0
26	3540.5
27	4338.0
28	5743.5
29	7149.0
30	7661.0
31	8173.0
32	9317.5
33	10462.0
34	13613.5
35	16765.0
36	17920.0
37	19075.0
38	21718.5
39	27305.5
40	30249.0
41	37073.0
42	43897.0
43	55205.0
44	66513.0
45	72414.5
46	78316.0
47	89163.0
48	100010.0
49	101477.5
50	102945.0
51	93542.5
52	84140.0
53	75279.5
54	66419.0
55	59692.5
56	52966.0
57	49805.0
58	46644.0
59	47479.5
60	48315.0
61	50054.0
62	51793.0
63	50427.0
64	39195.0
65	29329.0
66	23719.5
67	18110.0
68	15388.5
69	12667.0
70	10761.0
71	8855.0
72	7731.5
73	6608.0
74	5503.5
75	4399.0
76	3646.0
77	2893.0
78	2121.0
79	1349.0
80	966.0
81	583.0
82	412.0
83	241.0
84	143.0
85	45.0
86	41.0
87	37.0
88	19.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1014698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.254044247583344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47033240729634	18.049149370033515
2	10.675272953230856	5.391876310318435
3	3.7832936838439903	2.866303982801962
4	1.805277874177765	1.8236226925467391
5	1.0442247562302724	1.318544909913062
6	0.7377783650681522	1.1179132485804486
7	0.568357509735648	1.0047327989517236
8	0.5043946449010333	1.0190403744459826
9	0.43672235533825143	0.9926105117058915
>10	8.489910050569595	51.483260809884946
>50	0.37312500278558414	6.23872216288908
>100	0.09798450424519717	4.422237385939784
>500	0.008622636373577328	1.466094725827924
>1k	0.004311318186788664	2.296340645369149
>5k	3.919380169807876E-4	0.5095500707913956
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	5148	0.5073430715345847	TruSeq Adapter, Index 14 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4681	0.46131952561254674	No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	4216	0.41549308267090307	Illumina PCR Primer Index 3 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	3189	0.3142807022384985	TruSeq Adapter, Index 14 (96% over 26bp)
TGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTTG	2352	0.23179310494353986	TruSeq Adapter, Index 14 (96% over 28bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2101	0.20705668090407198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1203	0.11855744270709119	No Hit
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1142	0.1125458018050691	TruSeq Adapter, Index 14 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1140	0.11234869882467494	Illumina PCR Primer Index 3 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1139	0.11225014733447784	Illumina PCR Primer Index 3 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1030	0.10150803490299577	TruSeq Adapter, Index 14 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42327865039647267	0.0
2	0.0	0.0	0.0	1.4373734845244595	0.0
3	0.0	0.0	0.0	2.19996491566949	0.0
4	0.0	0.0	0.0	2.6800092244194826	0.0
5	0.0	0.0	0.0	5.1285209983660165	0.0
6	0.0	0.0	0.0	5.512181949703262	0.0
7	0.0	0.0	0.0	6.721802940382261	0.0
8	0.0	0.0	0.0	8.2544757159273	0.0
9	0.0	0.0	0.0	9.320901391349938	0.0
10	0.0	0.0	0.0	10.253789797555529	0.0
11	0.0	0.0	0.0	11.093349942544481	0.0
12	0.0	0.0	0.0	11.837118039061869	0.0
13	0.0	0.0	0.0	12.209839774987238	0.0
14	0.0	0.0	0.0	12.476520107460544	0.0
15	0.0	0.0	0.0	13.260103006017554	0.0
16	0.0	0.0	0.0	14.086851457280886	0.0
17	0.0	0.0	0.0	15.00032521991765	0.0
18	0.0	0.0	0.0	15.532897472942688	0.0
19	0.0	0.0	0.0	16.086756847850296	0.0
20	0.0	0.0	0.0	16.781544853739735	0.0
21	0.0	0.0	0.0	17.448836993864184	0.0
22	0.0	0.0	0.0	18.16964259316565	0.0
23	0.0	0.0	0.0	18.70320036109266	0.0
24	0.0	0.0	0.0	19.16235175392087	0.0
25	0.0	0.0	0.0	19.587699985611483	0.0
26	0.0	0.0	0.0	19.99363357373327	0.0
27	0.0	0.0	0.0	20.418390496482697	0.0
28	0.0	0.0	0.0	20.83940246260464	0.0
29	0.0	0.0	0.0	21.31915111688404	0.0
30	0.0	0.0	0.0	21.811612913398864	0.0
31	9.855149019708327E-5	0.0	0.0	22.28239338207033	0.0
32	9.855149019708327E-5	0.0	0.0	22.688031315721524	0.0
33	9.855149019708327E-5	0.0	0.0	23.09613303662765	0.0
34	9.855149019708327E-5	0.0	0.0	23.591649929338583	0.0
35	9.855149019708327E-5	0.0	0.0	24.026656207068505	0.0
36	9.855149019708327E-5	0.0	0.0	24.529170255583434	0.0
37	9.855149019708327E-5	0.0	0.0	24.954222832803456	0.0
38	9.855149019708327E-5	0.0	0.0	25.391397243317716	0.0
39	9.855149019708327E-5	0.0	0.0	25.838131148381095	0.0
40	9.855149019708327E-5	0.0	0.0	26.306447829797634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	20	6.3111034E-4	46.0	18
CGACATA	25	3.4168705E-5	46.0	30
TCGTATA	30	1.8611863E-6	46.0	28
CGTAAGT	25	3.4168705E-5	46.0	16
CGGTCTA	75	0.0	46.0	30
CTAGCGG	375	0.0	43.54667	1
TCGGCGT	585	0.0	43.247864	4
ACGTAGG	75	0.0	42.93333	1
CATGCGG	275	0.0	42.65455	1
TTAATCG	180	0.0	42.166668	19
CGTTGAT	180	0.0	42.166668	24
GTCGGCG	605	0.0	41.81818	3
CGTAGGG	355	0.0	41.46479	2
CGTTTTT	1180	0.0	41.322033	1
TTACGGG	285	0.0	41.157898	2
TAATCGT	185	0.0	41.027027	20
CACGACG	85	0.0	40.588234	25
CACACGG	120	0.0	40.25	1
TTGAGGG	1070	0.0	40.196262	2
TAGGGTA	345	0.0	40.0	4
>>END_MODULE