FastQCFastQC Report
Thu 26 May 2016
SRR1544850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544850_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3103420
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT160610.5175258263464177Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT148970.48001881794923024Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT112920.3638566484716861Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.18669725657500436No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC57260.18450612550025455No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC48870.15747143473973874Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT47780.15395918051697804Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC46850.15096248654709965Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC41560.13391677568617846Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC34000.1095565537374896Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA405.6170393E-946.011
ACGTCGA253.418509E-546.028
CGGTCTA2600.044.23076630
TACGGGA7150.044.0699273
TACGCGG3200.043.1251
GCGTAGG5250.042.933331
CGTTTTT46850.042.4162251
CTTGCGG6900.042.3333321
AATGCGG6850.042.3065721
ACGGGTA2300.042.04
GGCGATA10300.041.533987
GCTCGAC508.731149E-1041.39999830
CACGACG2700.040.8888925
CGTAGGG12150.040.888892
ACGGGAT16150.040.8730664
AATCGGG5200.040.6923032
ATAGCGG6150.040.3902441
TTACGGG9750.040.3384632
GCGATAG5200.040.2499968
AGGGCGA19500.039.6307685
Produced by FastQC (version 0.11.5)