##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544850_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3103420
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85625406809262	34.0	31.0	34.0	31.0	34.0
2	33.18359326162749	34.0	33.0	34.0	31.0	34.0
3	33.24412132421651	34.0	33.0	34.0	31.0	34.0
4	36.54319847136385	37.0	37.0	37.0	35.0	37.0
5	36.53925604655509	37.0	37.0	37.0	35.0	37.0
6	36.65546654980634	37.0	37.0	37.0	35.0	37.0
7	36.63003074028008	37.0	37.0	37.0	35.0	37.0
8	36.52125590477602	37.0	37.0	37.0	35.0	37.0
9	38.29612685360022	39.0	39.0	39.0	37.0	39.0
10	38.12999658441333	39.0	39.0	39.0	35.0	39.0
11	37.72225963614335	39.0	37.0	39.0	35.0	39.0
12	37.68564164695723	39.0	37.0	39.0	35.0	39.0
13	37.67286348608954	39.0	37.0	39.0	35.0	39.0
14	39.062141443955376	41.0	38.0	41.0	35.0	41.0
15	39.121342905568696	41.0	38.0	41.0	35.0	41.0
16	39.100100534249314	41.0	38.0	41.0	35.0	41.0
17	39.13102931604488	41.0	38.0	41.0	35.0	41.0
18	39.11298438496884	41.0	38.0	41.0	35.0	41.0
19	39.05346424267421	41.0	38.0	41.0	35.0	41.0
20	38.99801251522514	41.0	38.0	41.0	35.0	41.0
21	38.944402626779485	41.0	38.0	41.0	35.0	41.0
22	38.91374064741479	41.0	37.0	41.0	35.0	41.0
23	38.88069871303272	41.0	37.0	41.0	35.0	41.0
24	38.82553956602716	41.0	37.0	41.0	35.0	41.0
25	38.794788974743994	41.0	37.0	41.0	35.0	41.0
26	38.73284859928724	41.0	37.0	41.0	35.0	41.0
27	38.69810789387192	41.0	36.0	41.0	35.0	41.0
28	38.73338445972508	41.0	37.0	41.0	35.0	41.0
29	38.727044357515254	41.0	36.0	41.0	35.0	41.0
30	38.65997802424422	41.0	36.0	41.0	35.0	41.0
31	38.63993819721468	41.0	36.0	41.0	35.0	41.0
32	38.56353184551237	41.0	36.0	41.0	35.0	41.0
33	38.4768458668179	40.0	36.0	41.0	35.0	41.0
34	38.403181006760285	40.0	36.0	41.0	35.0	41.0
35	38.347123173788916	40.0	36.0	41.0	35.0	41.0
36	38.284210322805166	40.0	36.0	41.0	35.0	41.0
37	38.23033427637896	40.0	35.0	41.0	35.0	41.0
38	38.17520606298857	40.0	36.0	41.0	35.0	41.0
39	38.10075110684342	40.0	35.0	41.0	35.0	41.0
40	37.996438767553215	40.0	35.0	41.0	35.0	41.0
41	37.95234579915061	40.0	35.0	41.0	35.0	41.0
42	37.87797462154655	40.0	35.0	41.0	35.0	41.0
43	37.88113532812188	40.0	35.0	41.0	35.0	41.0
44	37.85805111779907	40.0	35.0	41.0	35.0	41.0
45	37.82061725451276	40.0	35.0	41.0	35.0	41.0
46	37.7794852130875	40.0	35.0	41.0	35.0	41.0
47	37.667332491251585	40.0	35.0	41.0	35.0	41.0
48	37.64049403561233	40.0	35.0	41.0	34.0	41.0
49	37.59275670067216	39.0	35.0	41.0	34.0	41.0
50	37.49435429300578	39.0	35.0	41.0	34.0	41.0
51	37.41575133240103	39.0	35.0	41.0	34.0	41.0
52	37.138205914765	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	3.0
13	10.0
14	14.0
15	27.0
16	37.0
17	88.0
18	157.0
19	327.0
20	630.0
21	1173.0
22	1786.0
23	3206.0
24	5234.0
25	8880.0
26	12895.0
27	15218.0
28	15916.0
29	17673.0
30	21206.0
31	25977.0
32	33459.0
33	46464.0
34	126709.0
35	425131.0
36	116423.0
37	181551.0
38	332140.0
39	1696868.0
40	14211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.694601439702005	22.738688285826605	24.94970065282817	18.61700962164322
2	26.82917555471061	24.009060971444406	26.210793253894092	22.950970219950893
3	26.83214002616468	24.779469101829594	26.237312384401722	22.151078487603996
4	25.8488055113391	27.260248371151825	23.62548414329997	23.2654619742091
5	24.55175258263464	35.05539050466904	20.8262497502755	19.566607162420812
6	86.35792126106038	2.699473484091744	7.897931958935627	3.044673295912252
7	87.56085222109802	2.6877444883386716	5.615836722067912	4.135566568495402
8	77.0508986859658	5.175741601201255	11.008435854637787	6.764923858195153
9	44.77550573238556	24.23697082573419	15.1522191646635	15.835304277216746
10	33.62235855926687	23.28460214859735	27.76572297658712	15.327316315548654
11	32.46698803255763	19.54856255356993	28.370668488312894	19.613780925559542
12	21.541557378633893	28.01560858665601	30.533862641859628	19.908971392850468
13	16.69419543600286	30.571176315161985	31.466124469134055	21.268503779701103
14	15.294062679237744	26.794310792609444	37.29781982458063	20.613806703572187
15	18.51396201609837	20.469900947986417	39.93436273530492	21.081774300610295
16	18.233046123309123	24.062937017870606	28.06945241056641	29.634564448253865
17	20.180768313666857	24.554459273962273	30.312300623183457	24.952471789187413
18	24.029328933885843	27.909145394435814	27.41372421393173	20.647801457746613
19	26.238246837360073	23.465853800001288	26.60954688698275	23.686352475655887
20	22.5958458732624	27.988992788600964	27.549961010755876	21.86520032738076
21	20.926365106882088	25.431427264115076	27.28915196782904	26.3530556611738
22	19.574630568856293	28.416875575977468	25.335017496826083	26.673476358340153
23	19.709127349826964	23.83029045375747	34.31627043713065	22.144311759284918
24	21.81064760812265	23.472201635614905	30.57098298006715	24.14616777619529
25	18.900825540854928	30.003737811833396	27.563494467394033	23.53194217991764
26	19.560098214228177	27.8100933808508	27.883528494370722	24.746279910550296
27	21.781035116097726	26.62849372627617	31.496316966443473	20.094154191182632
28	18.19631245529126	26.41224842270785	30.032738076058024	25.358701045942862
29	22.3678393514252	25.991776813966528	29.17558693312539	22.46479690148288
30	23.48202950293547	28.88851653981736	25.149995811072944	22.47945814617422
31	24.491689813173853	26.90589736484266	27.77384305057002	20.82856977141347
32	24.083398315406875	27.05057646080775	27.261376159205007	21.604649064580368
33	19.433334837050737	28.783148913134543	27.160036346997828	24.623479902816893
34	19.953116239503515	23.790624536801335	32.874667302524315	23.381591921170838
35	25.89407814604533	24.878327780319776	27.816312326401192	21.411281747233694
36	20.59263651068821	28.04190215955301	28.242261762829397	23.123199566929387
37	21.184950796218367	26.86487810222271	30.897107062531013	21.05306403902791
38	20.69143074414678	26.288675074595126	27.642955191369524	25.376938989888576
39	25.64419253597644	23.331324796514814	28.323236945047718	22.701245722461028
40	18.98534519981182	22.929800027066914	32.14650289035967	25.9383518827616
41	21.52547834324713	27.154655186858367	25.581487520219632	25.73837894967487
42	21.8134509669977	23.378691894748375	29.27138447261408	25.53647266563984
43	22.46850249080047	25.871747942592364	29.25227652074163	22.40747304586553
44	19.917671472117856	27.276842966791477	27.870510597985447	24.93497496310522
45	21.609256884340503	26.03978836251619	28.10969833280703	24.241256420336274
46	23.75144195758228	24.031358952381566	28.736458487732886	23.480740602303264
47	20.817614116039724	22.174246476467896	33.246611802463086	23.76152760502929
48	19.34784850261969	22.111348125616257	30.121124436911533	28.419678934852517
49	20.22832874699525	24.118230854992234	32.20611454459919	23.447325853413332
50	19.53702689291169	24.37633320659144	30.695941896359503	25.39069800413737
51	20.585160887021413	22.299656507981517	29.85354866566562	27.261633939331446
52	21.26396040497258	20.70689755173325	33.871986389209326	24.157155654084846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	906.0
2	1373.0
3	6144.0
4	10915.0
5	7454.0
6	3993.0
7	4101.0
8	4209.0
9	4338.0
10	4467.0
11	4341.0
12	4215.0
13	4113.0
14	3987.0
15	3963.0
16	4074.0
17	4185.0
18	4266.5
19	4348.0
20	4711.5
21	5075.0
22	6238.0
23	7401.0
24	9068.5
25	10736.0
26	12910.0
27	15084.0
28	20998.5
29	26913.0
30	30555.0
31	34197.0
32	40252.5
33	46308.0
34	53158.0
35	60008.0
36	67886.0
37	75764.0
38	83867.0
39	103509.5
40	115049.0
41	146309.0
42	177569.0
43	228721.0
44	279873.0
45	328322.0
46	376771.0
47	379943.5
48	383116.0
49	341218.5
50	299321.0
51	261358.0
52	223395.0
53	193992.0
54	164589.0
55	147401.5
56	130214.0
57	119037.5
58	107861.0
59	106853.5
60	105846.0
61	106455.0
62	107064.0
63	94093.5
64	65367.0
65	49611.0
66	39401.5
67	29192.0
68	24742.0
69	20292.0
70	16557.5
71	12823.0
72	10211.0
73	7599.0
74	6696.5
75	5794.0
76	5036.5
77	4279.0
78	2862.5
79	1446.0
80	1061.0
81	676.0
82	453.0
83	230.0
84	131.5
85	33.0
86	47.0
87	61.0
88	33.0
89	6.5
90	8.0
91	7.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3103420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.704318848040742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.18031166319156	13.702182741374274
2	13.330960198457017	5.520169009987892
3	6.121366988115206	3.8021620172342416
4	3.2766429548503866	2.7136264195363506
5	1.9109319731140817	1.9782272434134784
6	1.2093951511978729	1.502382169420512
7	0.8419714470919363	1.2202711711076402
8	0.6291011355071849	1.0420088397764193
9	0.45407106415698884	0.8461108882777915
>10	3.582101112188027	17.091472502722237
>50	1.9268794951681707	29.059741630116065
>100	0.5166815912118888	15.253121157021551
>500	0.012064499061933147	1.6940764938816875
>1k	0.006737317657962666	2.830154255978708
>5k	3.133636119982636E-4	0.3737076559595006
>10k+	4.700454179973953E-4	1.370585804191745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	16061	0.5175258263464177	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	14897	0.48001881794923024	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	11292	0.3638566484716861	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5794	0.18669725657500436	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5726	0.18450612550025455	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4887	0.15747143473973874	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	4778	0.15395918051697804	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4685	0.15096248654709965	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4156	0.13391677568617846	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3400	0.1095565537374896	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2222515805144E-5	0.0	0.0	0.48913778992208595	0.0
2	3.2222515805144E-5	0.0	0.0	1.645571659652899	0.0
3	3.2222515805144E-5	0.0	0.0	2.6350928975130663	0.0
4	3.2222515805144E-5	0.0	0.0	3.2250227168736427	0.0
5	3.2222515805144E-5	0.0	0.0	6.1655528417036685	0.0
6	3.2222515805144E-5	0.0	0.0	6.608676879056009	0.0
7	3.2222515805144E-5	0.0	0.0	8.039517693383429	0.0
8	3.2222515805144E-5	0.0	0.0	9.934427180336533	0.0
9	3.2222515805144E-5	0.0	0.0	11.210922143957312	0.0
10	6.4445031610288E-5	0.0	0.0	12.347313608857325	0.0
11	9.666754741543201E-5	0.0	0.0	13.43166571073203	0.0
12	9.666754741543201E-5	0.0	0.0	14.324616068724183	0.0
13	1.28890063220576E-4	0.0	0.0	14.72575416798242	0.0
14	1.28890063220576E-4	0.0	0.0	15.03351141643735	0.0
15	1.28890063220576E-4	0.0	0.0	15.99574018341056	0.0
16	1.6111257902572002E-4	0.0	0.0	17.134709449574984	0.0
17	1.6111257902572002E-4	0.0	0.0	18.380013017896385	0.0
18	1.6111257902572002E-4	0.0	0.0	19.08977837353629	0.0
19	1.6111257902572002E-4	0.0	0.0	19.915802566201158	0.0
20	1.6111257902572002E-4	0.0	0.0	20.870749044602405	0.0
21	1.6111257902572002E-4	0.0	0.0	21.778489537349117	0.0
22	1.6111257902572002E-4	0.0	0.0	22.740073854006226	0.0
23	1.6111257902572002E-4	0.0	0.0	23.45602593268072	0.0
24	1.6111257902572002E-4	0.0	0.0	24.089037255672775	0.0
25	1.6111257902572002E-4	0.0	0.0	24.681609321329372	0.0
26	1.6111257902572002E-4	0.0	0.0	25.24444000489782	0.0
27	1.6111257902572002E-4	0.0	0.0	25.84326323862062	0.0
28	1.6111257902572002E-4	0.0	0.0	26.418338478195025	0.0
29	1.9333509483086402E-4	0.0	0.0	27.0909512731116	0.0
30	1.9333509483086402E-4	0.0	0.0	27.738946065953044	0.0
31	2.2555761063600802E-4	0.0	0.0	28.35684502903249	0.0
32	2.57780126441152E-4	0.0	0.0	28.938493661831142	0.0
33	2.57780126441152E-4	0.0	0.0	29.49713541834492	0.0
34	2.57780126441152E-4	0.0	0.0	30.187696154564964	0.0
35	2.57780126441152E-4	0.0	0.0	30.770891468122265	0.0
36	2.57780126441152E-4	0.0	0.0	31.377190325511854	0.0
37	2.57780126441152E-4	0.0	0.0	31.922749740608747	0.0
38	2.57780126441152E-4	0.0	0.0	32.45448569642524	0.0
39	2.57780126441152E-4	0.0	0.0	33.017026377351435	0.0
40	2.57780126441152E-4	0.0	0.0	33.6118862416302	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCTA	40	5.6170393E-9	46.0	11
ACGTCGA	25	3.418509E-5	46.0	28
CGGTCTA	260	0.0	44.230766	30
TACGGGA	715	0.0	44.069927	3
TACGCGG	320	0.0	43.125	1
GCGTAGG	525	0.0	42.93333	1
CGTTTTT	4685	0.0	42.416225	1
CTTGCGG	690	0.0	42.333332	1
AATGCGG	685	0.0	42.306572	1
ACGGGTA	230	0.0	42.0	4
GGCGATA	1030	0.0	41.53398	7
GCTCGAC	50	8.731149E-10	41.399998	30
CACGACG	270	0.0	40.88889	25
CGTAGGG	1215	0.0	40.88889	2
ACGGGAT	1615	0.0	40.873066	4
AATCGGG	520	0.0	40.692303	2
ATAGCGG	615	0.0	40.390244	1
TTACGGG	975	0.0	40.338463	2
GCGATAG	520	0.0	40.249996	8
AGGGCGA	1950	0.0	39.630768	5
>>END_MODULE