FastQCFastQC Report
Thu 26 May 2016
SRR1544849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544849_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2032099
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT158590.7804245757711608Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT142710.7022787767721946Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT100590.4950054106615869Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA74880.3684859841966361No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTTG72790.3582010522125152Illumina Single End Adapter 2 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC39410.19393740167186735Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT37710.185571667522104Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC37520.18463667370536574Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33900.16682258098645783No Hit
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC33160.16318102612126673Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC28240.13896960728783392Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.1273559998799271No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT25820.12706073867464135Illumina Single End Adapter 2 (95% over 22bp)
CTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTTGAAA24910.12258261039447388Illumina Single End Adapter 2 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT22090.1087053337460429No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG21870.10762270932666174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA206.3126057E-446.045
TACGGGA4300.046.03
GCGATCG253.418089E-546.08
TCGGCGT10550.043.165884
TGATCGG800.043.1251
GCGTAGG2950.042.8813551
CGTTTTT25650.042.86161
GTCGGCG10700.042.77573
CTTGCGG4600.042.01
GTCCCCC10800.041.3148169
CTAGCGG5350.041.2710271
CGAAGGG10900.040.935782
ACGGGAT10950.040.5388154
GTTACGG2100.040.5238081
AATGCGG3750.040.481
ATAGCGG3300.040.424241
TTACGGG5300.040.358492
TACGCGG2000.040.251
TAGCGGG14550.039.6769752
ATTGCGG4350.039.6551741
Produced by FastQC (version 0.11.5)