FastQCFastQC Report
Thu 26 May 2016
SRR1544848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544848_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences733106
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT38990.5318466906559215Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT35000.4774207276983138TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT28580.38984812564622306Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.1827839357473544No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT11570.15782165198484258Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC11100.1514105736414652TruSeq Adapter, Index 23 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC10710.146090742675684TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC9670.13190452676693412Illumina PCR Primer Index 7 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9510.12972203201174182No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT7920.10803349038201844TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT7740.10557818378242709TruSeq Adapter, Index 23 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC7650.10435053048263143TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGG351.0188887E-746.0000041
CCGATGT351.0188887E-746.00000410
CGACCCC351.0188887E-746.00000419
GCTCGAT351.0188887E-746.0000048
GTGCAAC253.4159362E-546.08
TCCGCAA551.8189894E-1246.031
GGTCGGA206.3099514E-446.07
TAGACTT206.3099514E-446.028
CCTAGAC206.3099514E-446.026
GCGAAAC301.8604751E-646.08
TCGGGTG206.3099514E-446.04
ATATGCG253.4159362E-546.018
TCGATTG206.3099514E-446.08
TATGCGT206.3099514E-446.019
TGCGGAT206.3099514E-446.022
GGCCCCG253.4159362E-546.08
AAACGGC206.3099514E-446.036
AACGGCG206.3099514E-446.037
AGTGCGG1000.046.01
CTAACGG301.8604751E-646.01
Produced by FastQC (version 0.11.5)