FastQCFastQC Report
Thu 26 May 2016
SRR1544847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544847_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences510686
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT47120.9226804729324869Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT38520.7542795377198513TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT26920.5271340902237382Illumina PCR Primer Index 7 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTG19430.3804686245559894Illumina PCR Primer Index 7 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT10710.20971790885201477Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC10520.20599742307406116TruSeq Adapter, Index 23 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC9910.19405270557642074TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC8600.16840093521263555Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC7100.1390286790708968TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT6850.13413330304727367TruSeq Adapter, Index 23 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6350.1243425510000274No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.12081788026301876No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT5720.11200620342049714TruSeq Adapter, Index 23 (95% over 23bp)
GTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTGA5510.10789408756065372TruSeq Adapter, Index 23 (96% over 28bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG5510.10789408756065372No Hit
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC5210.10201963633230594TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT206.308143E-446.00000440
ATAGGCG206.308143E-446.0000049
TTGTCGT206.308143E-446.00000434
TTCACGG351.0180702E-746.0000041
GTTACTA206.308143E-446.0000048
ATATCTA206.308143E-446.00000411
CTAACGG351.0180702E-746.0000041
GGGCGTA206.308143E-446.0000046
TGTTCGG351.0180702E-746.0000041
AGACCGG206.308143E-446.0000041
GAAGTTA206.308143E-446.00000417
CGCAGAA206.308143E-446.00000429
AATTCCG206.308143E-446.00000412
TCATCGG206.308143E-446.0000041
ACCCGTG206.308143E-446.00000441
CCAACGG351.0180702E-746.0000041
ATAGTCG206.308143E-446.00000439
CATCCGT206.308143E-446.00000421
TGTCGTA206.308143E-446.00000435
TAGTCGC206.308143E-446.00000440
Produced by FastQC (version 0.11.5)