FastQCFastQC Report
Thu 26 May 2016
SRR1544846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544846_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1264795
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT62960.49778817911203005No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT59040.46679501421178926Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT49450.3909724500808431No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.21987752956012635No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT19490.15409611834328885No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19470.15393798995094066No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC18810.14871975300345114No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC18480.1461106345297064No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC16200.12808399780201535No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC15000.11859629426112532No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT14500.11464308445242113No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13980.11053174625136879No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT13580.10736917840440546No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13220.10452286734213845No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT13110.10365316118422353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGG1050.046.0000041
CTCGAGG1450.046.0000041
CTTCGTC253.417352E-546.022
CACGGTT206.311696E-446.037
CGACGAA206.311696E-446.012
ATTACGG501.6370905E-1146.01
ATAGCGG1250.046.01
ATCGTTC206.311696E-446.025
CGTTATC206.311696E-446.040
CGTTACC206.311696E-446.039
CCATACG206.311696E-446.011
AACGAAC253.417352E-546.024
CGTACAC253.417352E-546.014
ATGCGTA206.311696E-446.029
TAGGCGA206.311696E-446.019
CCGAACT206.311696E-446.036
TGTCGTT206.311696E-446.017
CGTTTAG301.8615519E-646.029
TATCGGA253.417352E-546.019
CAATACG206.311696E-446.015
Produced by FastQC (version 0.11.5)