##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544846_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1264795
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86437248724101	34.0	31.0	34.0	31.0	34.0
2	33.18573286580039	34.0	33.0	34.0	31.0	34.0
3	33.238708249162904	34.0	33.0	34.0	31.0	34.0
4	36.53930953237481	37.0	37.0	37.0	35.0	37.0
5	36.53752821603501	37.0	37.0	37.0	35.0	37.0
6	36.644710802936444	37.0	37.0	37.0	35.0	37.0
7	36.611438217260506	37.0	37.0	37.0	35.0	37.0
8	36.5000083017406	37.0	37.0	37.0	35.0	37.0
9	38.28253274246024	39.0	39.0	39.0	37.0	39.0
10	38.06800311512933	39.0	39.0	39.0	35.0	39.0
11	37.333002581446	39.0	35.0	39.0	35.0	39.0
12	37.219940780917064	39.0	35.0	39.0	35.0	39.0
13	37.20685328452437	39.0	35.0	39.0	35.0	39.0
14	38.458343842282744	40.0	35.0	41.0	35.0	41.0
15	38.52196442901814	41.0	35.0	41.0	35.0	41.0
16	38.487450535462266	41.0	35.0	41.0	35.0	41.0
17	38.56699544194909	41.0	36.0	41.0	35.0	41.0
18	38.553735585608734	40.0	36.0	41.0	35.0	41.0
19	38.48408002877937	40.0	35.0	41.0	35.0	41.0
20	38.3967472989694	40.0	35.0	41.0	35.0	41.0
21	38.332565356441165	40.0	35.0	41.0	35.0	41.0
22	38.319627291379234	40.0	35.0	41.0	35.0	41.0
23	38.281313572555234	40.0	35.0	41.0	35.0	41.0
24	38.23172371807289	40.0	35.0	41.0	35.0	41.0
25	38.19454377982203	40.0	35.0	41.0	35.0	41.0
26	38.136305092920196	40.0	35.0	41.0	35.0	41.0
27	38.10695883522626	40.0	35.0	41.0	35.0	41.0
28	38.141181772540214	40.0	35.0	41.0	35.0	41.0
29	38.147395427717534	40.0	35.0	41.0	35.0	41.0
30	38.093778833724045	40.0	35.0	41.0	35.0	41.0
31	38.034984325523105	40.0	35.0	41.0	35.0	41.0
32	37.985056866923095	40.0	35.0	41.0	35.0	41.0
33	37.88432038393574	40.0	35.0	41.0	35.0	41.0
34	37.81589901920864	40.0	35.0	41.0	35.0	41.0
35	37.7575883838883	40.0	35.0	41.0	35.0	41.0
36	37.70437817986314	40.0	35.0	41.0	35.0	41.0
37	37.624219735214005	40.0	35.0	41.0	35.0	41.0
38	37.582298317118585	40.0	35.0	41.0	35.0	41.0
39	37.47532604097897	40.0	35.0	41.0	34.0	41.0
40	37.395037931048115	39.0	35.0	41.0	34.0	41.0
41	37.372370226005	39.0	35.0	41.0	34.0	41.0
42	37.29142667388786	39.0	35.0	41.0	34.0	41.0
43	37.29607169541309	39.0	35.0	41.0	34.0	41.0
44	37.282289224736026	39.0	35.0	41.0	34.0	41.0
45	37.25871070015299	39.0	35.0	41.0	34.0	41.0
46	37.22251827371234	39.0	35.0	41.0	34.0	41.0
47	37.1379520001265	39.0	35.0	41.0	34.0	41.0
48	37.13400274352761	38.0	35.0	41.0	34.0	41.0
49	37.07728287983428	38.0	35.0	41.0	34.0	41.0
50	37.00133144106357	38.0	35.0	41.0	34.0	41.0
51	36.93622761000795	38.0	35.0	41.0	34.0	41.0
52	36.698529801272144	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	9.0
15	15.0
16	28.0
17	45.0
18	92.0
19	196.0
20	310.0
21	523.0
22	933.0
23	1437.0
24	2338.0
25	3952.0
26	5551.0
27	6815.0
28	7046.0
29	7798.0
30	9571.0
31	11805.0
32	15693.0
33	22321.0
34	71661.0
35	265881.0
36	48798.0
37	71814.0
38	119088.0
39	585426.0
40	5642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.21376191398606	22.666598144363313	24.419609501935096	19.700030439715526
2	28.276202862914545	23.787965638700342	25.77532327373211	22.160508224653007
3	27.991255499903144	24.016698358231967	25.833356393723882	22.158689748141004
4	26.830988421048467	25.96246822607616	23.152763886637757	24.053779466237614
5	24.66423412489771	34.688704493613585	20.8023434627746	19.8447179187141
6	85.48752959965844	3.079392312588206	8.400808036084898	3.032270051668452
7	86.63277448124003	3.0163781482374614	5.9047513628690815	4.446096007653414
8	75.60363537174008	5.599168244656249	11.592471507240305	7.204724876363364
9	51.112235579678924	19.785024450602666	14.87165904356042	14.231080926157993
10	43.45637039994624	18.99438248886183	23.851928573405175	13.697318537786757
11	40.359188643218864	17.51256132416716	23.6620954383912	18.46615459422278
12	21.386232551520205	31.746093240406548	27.019398400531315	19.848275807541935
13	15.214244205582721	33.787530785621385	30.838199075739546	20.160025933056342
14	16.00899750552461	24.446886649615156	40.83594574614858	18.708170098711648
15	18.262010839701297	18.781699801153547	43.027289007309484	19.929000351835676
16	17.477140564281168	22.04768361671259	26.94152016729984	33.533655651706404
17	20.651963361651493	23.683521835554377	30.838752525112767	24.825762277681363
18	24.426171830217545	27.7732755110512	26.719745097031534	21.08080756169972
19	28.471886748445403	22.691661494550498	24.878735289117998	23.9577164678861
20	21.649121003799035	29.974897117714725	26.75397989397491	21.622001984511325
21	20.724307101150778	23.71799382508628	28.224890199597564	27.332808874165377
22	20.426630402555354	27.334706414873555	24.511561162085556	27.727102020485532
23	21.29783878019758	21.745421194739066	35.919101514474676	21.03763851058867
24	20.839740827564942	21.49043916207765	31.69715250297479	25.972667507382617
25	16.47539719875553	30.492925731047322	28.365150083610384	24.66652698658676
26	17.27291774556351	28.421364727090157	28.13436169497824	26.171355832368093
27	19.193149878043478	27.723939452638568	32.59935404551726	20.483556623800695
28	17.540233792828086	25.797698441249374	29.492526456856645	27.1695413090659
29	18.64057021098281	29.519487347752005	27.81881648804747	24.021125953217716
30	26.30821595594543	25.914950644175537	22.080495258124834	25.6963381417542
31	25.79532651536415	31.050249249878437	21.61725813274088	21.537166102016535
32	22.540886072446522	28.681248739914373	22.463007839215052	26.314857348424052
33	16.17550670266723	29.464616795607196	27.138864400950354	27.221012100775226
34	17.207215398542846	28.639898165315326	27.973386991567807	26.179499444574024
35	23.280689756047423	26.878664131341445	23.26535130198965	26.575294810621486
36	19.75387315731008	30.982807490541948	26.354626639099614	22.90869271304836
37	19.1482414146166	31.116742238860844	25.01520009171447	24.71981625480809
38	18.8330915286667	28.61515107191284	25.228752485580667	27.323004913839792
39	23.561446716661592	24.38561189758024	26.56936499590843	25.483576389849738
40	15.260338631952214	23.909250115631387	30.94264287888551	29.88776837353089
41	19.22090141090058	27.238089967148827	23.239101988859858	30.301906633090738
42	20.108159820366144	22.781004036227216	27.384595922659404	29.726240220747236
43	21.37136848263948	27.037029716278134	26.593558639937697	24.998043161144693
44	17.701129432042347	30.95236777501492	24.171980439517867	27.174522353424862
45	20.006167007301578	28.594752509299926	24.830506129451805	26.56857435394669
46	21.756332053811093	24.70930071671694	26.591028585660126	26.943338643811842
47	19.54285081772145	21.229527314703173	32.852359473274326	26.375262394301053
48	18.989243316110517	21.474072873469613	29.30134923050771	30.23533457991216
49	18.304863634027647	24.719104677042523	31.533805873679132	25.44222581525069
50	17.132578797354512	26.21776651552228	29.05972904699971	27.589925640123496
51	18.55407398036836	21.920153068283792	28.996714882648966	30.529058068698877
52	19.702718622385447	20.734190125672537	33.46692547013548	26.096165781806533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	474.5
2	750.0
3	3052.5
4	5355.0
5	3536.0
6	1717.0
7	1779.5
8	1842.0
9	1885.5
10	1929.0
11	1918.0
12	1907.0
13	1828.0
14	1773.5
15	1798.0
16	1671.0
17	1544.0
18	1462.0
19	1380.0
20	1377.5
21	1375.0
22	1946.0
23	2517.0
24	2855.5
25	3194.0
26	3762.0
27	4330.0
28	6517.5
29	8705.0
30	9217.0
31	9729.0
32	11531.0
33	13333.0
34	15899.0
35	18465.0
36	19765.0
37	21065.0
38	24155.5
39	31463.5
40	35681.0
41	52760.0
42	69839.0
43	92713.5
44	115588.0
45	137230.5
46	158873.0
47	156864.5
48	154856.0
49	138968.0
50	123080.0
51	109544.0
52	96008.0
53	84108.0
54	72208.0
55	65788.5
56	59369.0
57	54955.5
58	50542.0
59	50245.0
60	49948.0
61	48505.0
62	47062.0
63	42993.5
64	31186.5
65	23448.0
66	18809.0
67	14170.0
68	11675.5
69	9181.0
70	7528.0
71	5875.0
72	4963.5
73	4052.0
74	3425.5
75	2799.0
76	2480.0
77	2161.0
78	1414.5
79	668.0
80	432.5
81	197.0
82	152.0
83	107.0
84	61.0
85	15.0
86	11.0
87	7.0
88	6.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1264795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.622756184491628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.43264191709041	15.255122169664666
2	13.042559695923591	5.9011729604511345
3	5.615387336575034	3.811066157904565
4	2.793568685596461	2.5279289303551797
5	1.642940455177283	1.8583920671556686
6	1.0664622306225315	1.4475789014005567
7	0.6872495439003402	1.0883235208692332
8	0.49926029575644865	0.9035715154796256
9	0.3685390029357659	0.7503631207102329
>10	5.02184092713375	30.171884856844787
>50	1.5962069661459128	23.257955687505376
>100	0.21474568601982713	7.895508314500682
>500	0.012281207533804248	1.8857836131947192
>1k	0.0056142663011676565	2.276895579106926
>5k	7.017832876459571E-4	0.968452604856692
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	6296	0.49778817911203005	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	5904	0.46679501421178926	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	4945	0.3909724500808431	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2781	0.21987752956012635	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1949	0.15409611834328885	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1947	0.15393798995094066	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1881	0.14871975300345114	No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1848	0.1461106345297064	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1620	0.12808399780201535	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1500	0.11859629426112532	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	1450	0.11464308445242113	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1398	0.11053174625136879	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1358	0.10736917840440546	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1322	0.10452286734213845	No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1311	0.10365316118422353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4775477448914646	0.0
2	0.0	0.0	0.0	1.6487256828181642	0.0
3	0.0	0.0	0.0	2.61749927853921	0.0
4	0.0	0.0	0.0	3.2264517174720013	0.0
5	0.0	0.0	0.0	6.25263382603505	0.0
6	0.0	0.0	0.0	6.721721701935887	0.0
7	0.0	0.0	0.0	8.336845101380066	0.0
8	7.906419617408354E-5	0.0	0.0	10.396151154930246	0.0
9	7.906419617408354E-5	0.0	0.0	11.808000506010856	0.0
10	7.906419617408354E-5	0.0	0.0	12.928419229993793	0.0
11	7.906419617408354E-5	0.0	0.0	14.005115453492463	0.0
12	7.906419617408354E-5	0.0	0.0	14.973414664036465	0.0
13	7.906419617408354E-5	0.0	0.0	15.385101933514918	0.0
14	7.906419617408354E-5	0.0	0.0	15.689736281373662	0.0
15	7.906419617408354E-5	0.0	0.0	16.658826133879405	0.0
16	7.906419617408354E-5	0.0	0.0	17.67914958550595	0.0
17	7.906419617408354E-5	0.0	0.0	19.070521309777472	0.0
18	7.906419617408354E-5	0.0	0.0	19.78494538640649	0.0
19	7.906419617408354E-5	0.0	0.0	20.56088140765895	0.0
20	7.906419617408354E-5	0.0	0.0	21.4927320237667	0.0
21	7.906419617408354E-5	0.0	0.0	22.280290481856742	0.0
22	7.906419617408354E-5	0.0	0.0	23.173083385054493	0.0
23	7.906419617408354E-5	0.0	0.0	23.85319360054396	0.0
24	1.5812839234816709E-4	0.0	0.0	24.449574832285073	0.0
25	1.5812839234816709E-4	0.0	0.0	24.99899193149878	0.0
26	1.5812839234816709E-4	0.0	0.0	25.477567510940506	0.0
27	1.5812839234816709E-4	0.0	0.0	26.02034321767559	0.0
28	1.5812839234816709E-4	0.0	0.0	26.527856292917033	0.0
29	1.5812839234816709E-4	0.0	0.0	27.2015623085164	0.0
30	1.5812839234816709E-4	0.0	0.0	27.819844322597735	0.0
31	1.5812839234816709E-4	0.0	0.0	28.394403836194797	0.0
32	1.5812839234816709E-4	0.0	0.0	28.96690768069134	0.0
33	1.5812839234816709E-4	0.0	0.0	29.497665629607962	0.0
34	1.5812839234816709E-4	0.0	0.0	30.208531817409146	0.0
35	1.5812839234816709E-4	0.0	0.0	30.74537770943117	0.0
36	1.5812839234816709E-4	0.0	0.0	31.317881553927712	0.0
37	1.5812839234816709E-4	0.0	0.0	31.875126008562653	0.0
38	1.5812839234816709E-4	0.0	0.0	32.387620128163064	0.0
39	1.5812839234816709E-4	0.0	0.0	33.048517743982224	0.0
40	1.5812839234816709E-4	0.0	0.0	33.662846548254855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGG	105	0.0	46.000004	1
CTCGAGG	145	0.0	46.000004	1
CTTCGTC	25	3.417352E-5	46.0	22
CACGGTT	20	6.311696E-4	46.0	37
CGACGAA	20	6.311696E-4	46.0	12
ATTACGG	50	1.6370905E-11	46.0	1
ATAGCGG	125	0.0	46.0	1
ATCGTTC	20	6.311696E-4	46.0	25
CGTTATC	20	6.311696E-4	46.0	40
CGTTACC	20	6.311696E-4	46.0	39
CCATACG	20	6.311696E-4	46.0	11
AACGAAC	25	3.417352E-5	46.0	24
CGTACAC	25	3.417352E-5	46.0	14
ATGCGTA	20	6.311696E-4	46.0	29
TAGGCGA	20	6.311696E-4	46.0	19
CCGAACT	20	6.311696E-4	46.0	36
TGTCGTT	20	6.311696E-4	46.0	17
CGTTTAG	30	1.8615519E-6	46.0	29
TATCGGA	25	3.417352E-5	46.0	19
CAATACG	20	6.311696E-4	46.0	15
>>END_MODULE