FastQCFastQC Report
Thu 26 May 2016
SRR1544845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544845_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences876737
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT77150.8799674246666902No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT67820.7735501068165254Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT45020.5134949249318781No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA34210.3901968321172712No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG32530.3710348713468235Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC17670.20154276596060164No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT17460.19914752086429566No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC16920.19298831918808035No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC14040.1601392435815986No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC13570.15477845693748524No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12660.1443990615201594No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT12120.13823985984394407No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.13573055545733784No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT10380.11839354333169469No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT9380.1069876143016663No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG9250.10550484352776261No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC9040.10310959843145663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG351.01919795E-746.0000041
CACGTTC351.01919795E-746.00000446
TGGCGAA206.3106325E-446.02
ATAGCGG800.046.01
TAAACGG301.8608935E-646.01
GTAGGGT1150.046.03
ACGGGTA253.4164874E-546.04
CGTTCAC253.4164874E-546.020
TATGACG206.3106325E-446.017
CCACTAG206.3106325E-446.017
CGAATAT950.046.014
GCGATAC253.4164874E-546.08
TAAGGCG405.6079443E-946.01
CGGTCTA453.092282E-1046.030
CCAACGG453.092282E-1046.01
GATCTCG206.3106325E-446.08
AATAGGC206.3106325E-446.042
CACAACG206.3106325E-446.011
ATTGCGG1300.046.01
TGCGTGT206.3106325E-446.019
Produced by FastQC (version 0.11.5)