##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544845_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	876737
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86851815310635	34.0	33.0	34.0	31.0	34.0
2	33.22004546403311	34.0	33.0	34.0	31.0	34.0
3	33.30957630395432	34.0	34.0	34.0	31.0	34.0
4	36.55833619432053	37.0	37.0	37.0	35.0	37.0
5	36.56692371828724	37.0	37.0	37.0	35.0	37.0
6	36.49310340501199	37.0	37.0	37.0	35.0	37.0
7	36.34678814741479	37.0	37.0	37.0	35.0	37.0
8	36.391808489889215	37.0	37.0	37.0	35.0	37.0
9	38.23911845855713	39.0	39.0	39.0	37.0	39.0
10	38.09824382910725	39.0	39.0	39.0	35.0	39.0
11	37.46805598486205	39.0	35.0	39.0	35.0	39.0
12	37.40400713098683	39.0	35.0	39.0	35.0	39.0
13	37.39735861495522	39.0	35.0	39.0	35.0	39.0
14	38.630598457690276	41.0	35.0	41.0	35.0	41.0
15	38.685184952842185	41.0	35.0	41.0	35.0	41.0
16	38.666548805400026	41.0	35.0	41.0	35.0	41.0
17	38.75479647830535	41.0	36.0	41.0	35.0	41.0
18	38.75675487631981	41.0	36.0	41.0	35.0	41.0
19	38.696926216185695	41.0	35.0	41.0	35.0	41.0
20	38.621168035568246	41.0	35.0	41.0	35.0	41.0
21	38.560935605546476	41.0	35.0	41.0	35.0	41.0
22	38.550391964751114	40.0	35.0	41.0	35.0	41.0
23	38.523890288649845	40.0	35.0	41.0	35.0	41.0
24	38.457456455014444	40.0	35.0	41.0	35.0	41.0
25	38.41139018884797	40.0	35.0	41.0	35.0	41.0
26	38.35800815980163	40.0	35.0	41.0	35.0	41.0
27	38.33141637686102	40.0	35.0	41.0	35.0	41.0
28	38.40639895430443	40.0	35.0	41.0	35.0	41.0
29	38.434304700269294	40.0	36.0	41.0	35.0	41.0
30	38.407596576852576	40.0	35.0	41.0	35.0	41.0
31	38.3760785731639	40.0	35.0	41.0	35.0	41.0
32	38.356259630881326	40.0	35.0	41.0	35.0	41.0
33	38.2744334960199	40.0	35.0	41.0	35.0	41.0
34	38.22615105784289	40.0	35.0	41.0	35.0	41.0
35	38.15832912264453	40.0	35.0	41.0	35.0	41.0
36	38.12110473266213	40.0	35.0	41.0	35.0	41.0
37	38.075017935823396	40.0	35.0	41.0	35.0	41.0
38	38.02390454606113	40.0	35.0	41.0	35.0	41.0
39	37.98321845661812	40.0	35.0	41.0	35.0	41.0
40	37.93852318312105	40.0	35.0	41.0	35.0	41.0
41	37.90368149171302	40.0	35.0	41.0	35.0	41.0
42	37.84684346617058	40.0	35.0	41.0	35.0	41.0
43	37.77274028585539	40.0	35.0	41.0	35.0	41.0
44	37.65574739060859	40.0	35.0	41.0	35.0	41.0
45	37.644153263749565	40.0	35.0	41.0	35.0	41.0
46	37.60494994508045	39.0	35.0	41.0	35.0	41.0
47	37.53278006973585	39.0	35.0	41.0	35.0	41.0
48	37.48955159871204	39.0	35.0	41.0	35.0	41.0
49	37.4594901321605	39.0	35.0	41.0	35.0	41.0
50	37.39454020989191	39.0	35.0	41.0	35.0	41.0
51	37.32486823300488	39.0	35.0	41.0	35.0	41.0
52	36.943221285288516	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	10.0
14	7.0
15	12.0
16	20.0
17	32.0
18	73.0
19	95.0
20	194.0
21	316.0
22	493.0
23	735.0
24	1282.0
25	2071.0
26	3103.0
27	3567.0
28	3663.0
29	4213.0
30	4974.0
31	6351.0
32	8605.0
33	12668.0
34	43316.0
35	174751.0
36	31506.0
37	48535.0
38	83415.0
39	437945.0
40	4783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03480633302803	22.487359379152473	23.29832093318749	22.179513354632004
2	27.93893721834484	24.12103059412344	25.679536736786517	22.260495450745207
3	28.02687693116636	23.739274149488388	25.93788102931666	22.295967890028596
4	26.89312758558154	25.322531158146628	23.63148812015462	24.152853136117216
5	24.44712610509195	33.589548519111204	21.19404108643755	20.769284289359295
6	82.97790557487593	3.078688363785263	9.740549332353945	4.202856728984861
7	84.31456639790495	2.9981625048332625	7.088214595711142	5.599056501550636
8	72.00209412856992	5.65677050244258	13.609440459339575	8.731694909647933
9	49.27156034249724	19.01539458241183	16.04540472228274	15.667640352808196
10	40.938046415287594	18.696028569571034	26.265117133188177	14.100807881953198
11	37.7278476897861	17.448333992976227	24.533583047139565	20.290235270098105
12	20.68716160034309	29.890149497511796	28.77921200998703	20.64347689215808
13	14.956252559205327	32.2199245611854	31.753079885986335	21.070742993622947
14	15.627947719783696	24.528678497656653	39.418548549907214	20.424825232652438
15	17.61360590462134	18.822406263223748	42.53179687865346	21.03219095350145
16	16.91202720998429	23.22714793604011	26.957342966020597	32.903481887954996
17	19.59766725939478	23.99328418898712	30.926948446341378	25.48210010527672
18	23.2231558608796	27.945666716472555	27.342179011493755	21.488998411154085
19	27.01209142536473	22.432724979098634	25.20596256345974	25.349221032076898
20	20.749666091427645	30.454857043788504	27.121018047601503	21.674458817182348
21	20.17640409837842	23.62749604499411	28.272674701763474	27.923425154864002
22	19.835937116832074	27.427153182767466	24.31915158137503	28.417758119025432
23	20.267537471328346	21.84303844824617	35.912365966076486	21.977058114349003
24	21.30102870073922	21.646058053897576	30.583059686086024	26.469853559277183
25	16.34378382570828	30.167997928683288	27.89627904377253	25.5919392018359
26	16.99095623887209	28.189867657005465	27.222188638097855	27.596987466024586
27	20.025275538730543	27.11314795657078	31.24563010344037	21.615946401258302
28	17.463161700715265	26.131439644956238	28.14903443107796	28.25636422325053
29	18.563491674242105	30.06979287973474	26.258957931511954	25.107757514511192
30	25.02563482549499	26.491867002305135	21.027058285437935	27.455439886761933
31	24.723491765489534	31.68361777819346	20.71579048220846	22.877099974108543
32	21.2933867282891	28.365176786197	21.98720939118573	28.35422709432817
33	15.335271580873169	29.910223932604644	26.770057611347532	27.98444687517465
34	17.045476579635626	28.612571386858317	27.582387876866154	26.759564156639904
35	22.078570882716253	26.659648218336855	23.043854656527557	28.217926242419335
36	19.50972754657326	30.983065617169114	25.908111554548285	23.59909528170934
37	18.700020644731545	31.035989127868447	25.35663488594641	24.907355341453595
38	18.192456802895283	28.446272941600505	24.68733497046435	28.67393528503987
39	23.855956803465578	23.773491936578473	26.66135910769136	25.709192152264592
40	15.260220567855582	23.207757856689064	31.07750670953775	30.454514865917602
41	18.881488975599297	27.25138781641473	22.961161671059852	30.905961536926124
42	19.60257180887769	21.666132488990428	27.936770091829132	30.79452561030275
43	22.077772467684152	25.987382761306982	26.41670192999725	25.518142841011617
44	17.707590759828772	29.394789999737664	25.01343048143286	27.884188759000704
45	19.4051351773679	27.39783994516029	25.104563854382782	28.092461023089022
46	21.897900966880606	23.347936724468113	26.806556584243623	27.94760572440766
47	18.51056816354277	20.43337967942496	33.89739454363167	27.1586576134006
48	18.77507165774913	20.296622590354918	29.051243417353206	31.877062334542742
49	18.325449935385414	23.13453179231628	32.145786022490206	26.3942322498081
50	17.36997526053993	24.33922601646788	29.979686040397517	28.31111268259467
51	18.38544512208336	20.395854172916163	29.404028802251986	31.814671902748486
52	19.29233053926092	19.839130776960477	34.99156531548229	25.87697336829631
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	261.5
2	386.0
3	1479.5
4	2573.0
5	1771.0
6	969.0
7	987.5
8	1006.0
9	1033.5
10	1061.0
11	1126.5
12	1192.0
13	1117.5
14	1024.0
15	1005.0
16	932.0
17	859.0
18	836.5
19	814.0
20	833.5
21	853.0
22	1235.5
23	1618.0
24	1703.0
25	1788.0
26	2256.0
27	2724.0
28	3804.0
29	4884.0
30	5271.0
31	5658.0
32	6758.5
33	7859.0
34	9547.0
35	11235.0
36	11975.5
37	12716.0
38	14776.5
39	19755.0
40	22673.0
41	33846.5
42	45020.0
43	61816.0
44	78612.0
45	98736.0
46	118860.0
47	118733.5
48	118607.0
49	104563.0
50	90519.0
51	79421.0
52	68323.0
53	59062.0
54	49801.0
55	44976.0
56	40151.0
57	36696.5
58	33242.0
59	32947.0
60	32652.0
61	31673.5
62	30695.0
63	29392.0
64	21968.0
65	15847.0
66	12702.0
67	9557.0
68	7905.5
69	6254.0
70	5135.0
71	4016.0
72	3345.5
73	2675.0
74	2268.0
75	1861.0
76	1657.5
77	1454.0
78	942.5
79	431.0
80	265.5
81	100.0
82	84.0
83	68.0
84	35.5
85	3.0
86	3.0
87	3.0
88	3.5
89	2.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	876737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.343098773410077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44525577047926	18.030601333330516
2	12.921161351495156	6.807668594992116
3	5.125646022353085	4.0507619833315145
4	2.4607692494256397	2.5929714958475922
5	1.3814265705141164	1.8195528297633254
6	0.8871276108085944	1.4021814165690059
7	0.6003508466794056	1.1070571156943165
8	0.4868238041112828	1.0259558045560608
9	0.394854902702374	0.9361531532748478
>10	6.794917742665571	43.60844179413545
>50	0.359947621118915	6.384179986512583
>100	0.12563389174802045	6.021270703282509
>500	0.009998545018008549	1.821990088994175
>1k	0.005216632183308807	2.7310383181856115
>5k	8.694386972181346E-4	1.6601753815303928
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	7715	0.8799674246666902	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	6782	0.7735501068165254	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	4502	0.5134949249318781	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3421	0.3901968321172712	No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG	3253	0.3710348713468235	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1767	0.20154276596060164	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1746	0.19914752086429566	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1692	0.19298831918808035	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1404	0.1601392435815986	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1357	0.15477845693748524	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1266	0.1443990615201594	No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1212	0.13823985984394407	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1190	0.13573055545733784	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1038	0.11839354333169469	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	938	0.1069876143016663	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	925	0.10550484352776261	No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	904	0.10310959843145663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1405929030028389E-4	0.0	0.0	0.7888340517167635	0.0
2	1.1405929030028389E-4	0.0	0.0	2.5553843398875604	0.0
3	1.1405929030028389E-4	0.0	0.0	3.830453146154434	0.0
4	1.1405929030028389E-4	0.0	0.0	4.6443802417372595	0.0
5	1.1405929030028389E-4	0.0	0.0	8.545207970006969	0.0
6	1.1405929030028389E-4	0.0	0.0	9.150634682920876	0.0
7	1.1405929030028389E-4	0.0	0.0	11.247842853672196	0.0
8	1.1405929030028389E-4	0.0	0.0	13.753611402279132	0.0
9	1.1405929030028389E-4	0.0	0.0	15.537042465414372	0.0
10	1.1405929030028389E-4	0.0	0.0	16.9553697402984	0.0
11	1.1405929030028389E-4	0.0	0.0	18.253250404625334	0.0
12	1.1405929030028389E-4	0.0	0.0	19.471403625032366	0.0
13	1.1405929030028389E-4	0.0	0.0	19.99653259757487	0.0
14	1.1405929030028389E-4	0.0	0.0	20.371787662662804	0.0
15	1.1405929030028389E-4	0.0	0.0	21.512608684246246	0.0
16	1.1405929030028389E-4	0.0	0.0	22.68764749291977	0.0
17	1.1405929030028389E-4	0.0	0.0	24.211593670621863	0.0
18	1.1405929030028389E-4	0.0	0.0	25.0300831378167	0.0
19	1.1405929030028389E-4	0.0	0.0	25.896135329066755	0.0
20	1.1405929030028389E-4	0.0	0.0	26.945024562668166	0.0
21	1.1405929030028389E-4	0.0	0.0	27.82636069881846	0.0
22	2.2811858060056779E-4	0.0	0.0	28.780124484309432	0.0
23	2.2811858060056779E-4	0.0	0.0	29.500751080426628	0.0
24	3.421778709008517E-4	0.0	0.0	30.145186070623232	0.0
25	3.421778709008517E-4	0.0	0.0	30.744453581860924	0.0
26	3.421778709008517E-4	0.0	0.0	31.275171459628144	0.0
27	3.421778709008517E-4	0.0	0.0	31.854136417192386	0.0
28	3.421778709008517E-4	0.0	0.0	32.3828012277342	0.0
29	3.421778709008517E-4	0.0	0.0	33.07787854282413	0.0
30	3.421778709008517E-4	0.0	0.0	33.739080248694876	0.0
31	3.421778709008517E-4	0.0	0.0	34.32169510354873	0.0
32	3.421778709008517E-4	0.0	0.0	34.91765489536771	0.0
33	3.421778709008517E-4	0.0	0.0	35.466166022421774	0.0
34	3.421778709008517E-4	0.0	0.0	36.16706036131702	0.0
35	3.421778709008517E-4	0.0	0.0	36.70564833011496	0.0
36	3.421778709008517E-4	0.0	0.0	37.256782820845935	0.0
37	3.421778709008517E-4	0.0	0.0	37.81943729989723	0.0
38	3.421778709008517E-4	0.0	0.0	38.34011796011803	0.0
39	3.421778709008517E-4	0.0	0.0	38.98991373695875	0.0
40	3.421778709008517E-4	0.0	0.0	39.59819193213016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	35	1.01919795E-7	46.000004	1
CACGTTC	35	1.01919795E-7	46.000004	46
TGGCGAA	20	6.3106325E-4	46.0	2
ATAGCGG	80	0.0	46.0	1
TAAACGG	30	1.8608935E-6	46.0	1
GTAGGGT	115	0.0	46.0	3
ACGGGTA	25	3.4164874E-5	46.0	4
CGTTCAC	25	3.4164874E-5	46.0	20
TATGACG	20	6.3106325E-4	46.0	17
CCACTAG	20	6.3106325E-4	46.0	17
CGAATAT	95	0.0	46.0	14
GCGATAC	25	3.4164874E-5	46.0	8
TAAGGCG	40	5.6079443E-9	46.0	1
CGGTCTA	45	3.092282E-10	46.0	30
CCAACGG	45	3.092282E-10	46.0	1
GATCTCG	20	6.3106325E-4	46.0	8
AATAGGC	20	6.3106325E-4	46.0	42
CACAACG	20	6.3106325E-4	46.0	11
ATTGCGG	130	0.0	46.0	1
TGCGTGT	20	6.3106325E-4	46.0	19
>>END_MODULE