FastQCFastQC Report
Thu 26 May 2016
SRR1544844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544844_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1077024
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT61950.5751960959087262RNA PCR Primer, Index 16 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT58240.5407493240633449RNA PCR Primer, Index 16 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT43660.4053762961642452Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT17560.16304186350536293Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC17470.16220622753067712RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16130.14976453635202186No Hit
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC15430.14326514543779897Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.14261520634637667No Hit
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC12850.11931024749680601Illumina PCR Primer Index 7 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC11610.1077970407344683RNA PCR Primer, Index 16 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT11380.10566152657693793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT253.4170123E-546.012
TCGTTCC206.311276E-446.013
CGAACAC206.311276E-446.018
ACACGAA206.311276E-446.032
TCCGCGT253.4170123E-546.043
ACGCCCG206.311276E-446.044
GATCGAC253.4170123E-546.08
CGGTTTA206.311276E-446.029
GGCCGTA253.4170123E-546.016
ACCGAGT253.4170123E-546.013
GTTAGTC253.4170123E-546.029
TTACTCG301.8612936E-646.038
CGATTCC206.311276E-446.039
ACTTACC206.311276E-446.04
ATCCGTG206.311276E-446.046
TGTTGCG206.311276E-446.032
ACGATAT206.311276E-446.028
CTAACGG1000.046.01
TCTACGG206.311276E-446.01
TACGATA206.311276E-446.023
Produced by FastQC (version 0.11.5)