##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544844_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1077024
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86000497667647	34.0	31.0	34.0	31.0	34.0
2	33.19170603440592	34.0	33.0	34.0	31.0	34.0
3	33.25462013845559	34.0	34.0	34.0	31.0	34.0
4	36.54839817868497	37.0	37.0	37.0	35.0	37.0
5	36.54265178863238	37.0	37.0	37.0	35.0	37.0
6	36.64264306087887	37.0	37.0	37.0	35.0	37.0
7	36.61306061889057	37.0	37.0	37.0	35.0	37.0
8	36.52065877826307	37.0	37.0	37.0	35.0	37.0
9	38.31991301957988	39.0	39.0	39.0	37.0	39.0
10	38.076017804617166	39.0	39.0	39.0	35.0	39.0
11	37.16815317021719	39.0	35.0	39.0	35.0	39.0
12	37.04675940369017	39.0	35.0	39.0	35.0	39.0
13	37.01444257509582	39.0	35.0	39.0	35.0	39.0
14	38.21416700092106	40.0	35.0	41.0	35.0	41.0
15	38.29731649433996	40.0	35.0	41.0	35.0	41.0
16	38.29102508393499	40.0	35.0	41.0	35.0	41.0
17	38.38636743470897	40.0	36.0	41.0	35.0	41.0
18	38.37099266125917	40.0	36.0	41.0	35.0	41.0
19	38.27996869150548	40.0	35.0	41.0	35.0	41.0
20	38.184893744243396	40.0	35.0	41.0	35.0	41.0
21	38.12338629408444	40.0	35.0	41.0	35.0	41.0
22	38.1150763585584	40.0	35.0	41.0	35.0	41.0
23	38.080552522506466	40.0	35.0	41.0	35.0	41.0
24	38.021576120866385	40.0	35.0	41.0	35.0	41.0
25	37.96794036158897	40.0	35.0	41.0	35.0	41.0
26	37.895301311762786	40.0	35.0	41.0	35.0	41.0
27	37.91770378375969	40.0	35.0	41.0	35.0	41.0
28	37.9244807915144	40.0	35.0	41.0	35.0	41.0
29	37.90245249873726	40.0	35.0	41.0	35.0	41.0
30	37.828249881153994	40.0	35.0	41.0	35.0	41.0
31	37.71384203137534	40.0	35.0	41.0	35.0	41.0
32	37.66986529548088	40.0	35.0	41.0	35.0	41.0
33	37.543732544492975	39.0	35.0	41.0	35.0	41.0
34	37.48962790058532	39.0	35.0	41.0	35.0	41.0
35	37.44550353566866	39.0	35.0	41.0	35.0	41.0
36	37.35250096562379	39.0	35.0	41.0	34.0	41.0
37	37.310170432599456	39.0	35.0	41.0	34.0	41.0
38	37.27619904477523	39.0	35.0	41.0	34.0	41.0
39	37.152259373978666	39.0	35.0	41.0	34.0	41.0
40	37.08334168969308	38.0	35.0	41.0	34.0	41.0
41	37.07313021808242	38.0	35.0	41.0	34.0	41.0
42	36.98156865585168	38.0	35.0	41.0	34.0	41.0
43	36.98575147814719	38.0	35.0	41.0	34.0	41.0
44	36.94476817601093	38.0	35.0	41.0	34.0	41.0
45	36.9395315239029	38.0	35.0	41.0	34.0	41.0
46	36.89746003803072	37.0	35.0	41.0	34.0	41.0
47	36.84425695249131	37.0	35.0	41.0	34.0	41.0
48	36.851050672965506	37.0	35.0	41.0	34.0	41.0
49	36.78798801140922	37.0	35.0	41.0	34.0	41.0
50	36.70237153489616	37.0	35.0	41.0	34.0	41.0
51	36.635177117687256	37.0	35.0	41.0	34.0	41.0
52	36.37663041863505	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	4.0
15	9.0
16	27.0
17	43.0
18	59.0
19	176.0
20	295.0
21	504.0
22	846.0
23	1408.0
24	2198.0
25	3509.0
26	4843.0
27	5646.0
28	5911.0
29	6831.0
30	8177.0
31	10246.0
32	13568.0
33	20040.0
34	65069.0
35	268067.0
36	40518.0
37	59442.0
38	104690.0
39	451449.0
40	3445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58423210624833	22.32726475918828	24.737517455507028	20.350985679056365
2	26.6145415515346	23.902438571471016	27.045451169147576	22.437568707846808
3	27.49576611106159	24.49063344920819	25.412247080844995	22.601353358885223
4	25.816694892592924	27.52037094809401	23.09447143239148	23.56846272692159
5	24.46593576373414	34.716496568321595	20.422850372879342	20.394717295064922
6	86.0989170157768	3.195471967198502	7.526573223995009	3.179037793029682
7	87.5137415693615	2.5930712778916716	5.393937368155212	4.4992497845916155
8	78.06436996761447	4.580492171019401	10.790660189559379	6.564477671806756
9	54.82421933030276	18.730687524140592	13.089309059036752	13.355784086519892
10	47.431440710699114	18.41036040051104	22.594946816412634	11.563252072377217
11	43.40432525180498	17.4100112903705	22.20396202870131	16.981701429123213
12	20.654135840984043	37.304182636598625	25.041967495617552	16.999714026799776
13	13.546587634073148	39.67460335145735	28.68348337641501	18.09532563805449
14	13.47091615414327	23.212110407938912	45.72145096116707	17.595522476750748
15	15.139588347149182	17.560890008022106	47.63979261372077	19.659729031107943
16	15.350354309653266	20.32498811539947	27.0007910687227	37.32386650622456
17	19.757498440146183	23.12204741955611	32.49361202721573	24.626842113081974
18	25.36628710223728	26.692627090946907	26.28390825088392	21.6571775559319
19	29.142154678075883	22.320579671390796	24.05127462340672	24.485991027126598
20	20.372340820631667	29.996267492646407	27.615540600766558	22.015851085955372
21	20.981240900852722	23.801790860742194	26.147606738568502	29.06936149983659
22	19.474310693169326	29.419678670113203	22.81109798853136	28.29491264818611
23	19.729551059215023	21.76135350744273	37.611139584633214	20.897955848709035
24	18.817593665507918	21.91083949846986	34.15782749502333	25.113739340998904
25	16.09054208634162	32.58228228897406	28.006061146269722	23.321114478414597
26	17.25281887868794	31.283425438987432	26.889837180972755	24.573918501351873
27	18.97097929108358	27.669671242237868	35.70198992780105	17.657359538877497
28	16.488026264967168	24.163156995572987	33.31244243396619	26.03637430549366
29	18.646659684463856	28.954693674421367	28.609854562201026	23.788792078913747
30	29.85077398460944	24.119332531122797	21.345578185815732	24.68431529845203
31	27.97653534183082	26.41937412722465	24.193889829753097	21.41020070119143
32	25.397112784859022	27.40802433371958	22.092172504976677	25.10269037644472
33	18.053265293995306	25.025997563656894	27.00914742846956	29.911589713878246
34	17.908885967257927	25.818366164542294	29.432770300383275	26.839977567816504
35	27.861496122649076	21.453375226550197	24.07922200433788	26.605906646462845
36	20.98346926345188	27.529841489140445	25.174183676501173	26.312505570906495
37	19.860374513474166	30.02059378435392	24.854970734171197	25.264060968000713
38	19.005054669162433	27.622782779213832	24.52025210208872	28.85191044953502
39	24.876604421071395	22.15382387022016	24.43251032474671	28.53706138396173
40	16.47539887690525	21.306860385655288	28.888307038654666	33.3294336987848
41	20.937137891077636	25.934241019698725	22.35790474492676	30.77071634429688
42	21.532482098820456	21.6867033603708	26.89652226877024	29.884292272038504
43	21.664883976587337	25.631555099979202	27.279614938942863	25.423945984490597
44	17.5453843182696	31.16922185578037	23.867620405859107	27.417773420090917
45	21.30853165760466	27.435136078676052	25.493953709480937	25.762378554238346
46	22.24490819146091	23.137645957750244	26.890208574739283	27.72723727604956
47	21.14613973319072	19.178124164364025	32.17672029592655	27.4990158065187
48	19.978663428113023	18.547219003476243	29.852630953442077	31.621486614968653
49	19.2031932436046	25.656902724544672	29.844738835903378	25.29516519594735
50	18.220113943607572	26.69021303146448	27.295677719345157	27.79399530558279
51	19.714695308553942	21.90294738093116	28.728793415931307	29.653563894583595
52	22.943685563181507	19.69668271087738	31.79056362718008	25.569068098761033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	321.5
2	443.0
3	1731.5
4	3020.0
5	2035.0
6	1050.0
7	1182.0
8	1314.0
9	1385.5
10	1457.0
11	1442.0
12	1427.0
13	1441.5
14	1462.0
15	1468.0
16	1303.5
17	1139.0
18	1280.5
19	1422.0
20	1318.0
21	1214.0
22	1473.0
23	1732.0
24	2129.5
25	2527.0
26	3234.0
27	3941.0
28	5194.0
29	6447.0
30	7165.0
31	7883.0
32	9104.5
33	10326.0
34	12206.0
35	14086.0
36	15864.5
37	17643.0
38	18824.5
39	23574.0
40	27142.0
41	40492.5
42	53843.0
43	73186.5
44	92530.0
45	98295.0
46	104060.0
47	101623.0
48	99186.0
49	107801.0
50	116416.0
51	120034.0
52	123652.0
53	106872.5
54	90093.0
55	78581.0
56	67069.0
57	56857.5
58	46646.0
59	42432.5
60	38219.0
61	37092.0
62	35965.0
63	33260.0
64	24647.0
65	18739.0
66	15405.0
67	12071.0
68	10096.5
69	8122.0
70	6316.0
71	4510.0
72	3961.5
73	3413.0
74	3015.0
75	2617.0
76	1788.0
77	959.0
78	671.5
79	384.0
80	411.5
81	439.0
82	277.0
83	115.0
84	88.5
85	62.0
86	35.5
87	9.0
88	5.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1077024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.673668056978435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.45674697961688	14.152520633171603
2	12.788116359208251	5.287545453685739
3	5.382143955224014	3.3380597269552275
4	2.7801470504491386	2.299033490822927
5	1.5938095764512599	1.6474945064793367
6	0.9951464811046948	1.2343996811057172
7	0.648948481670908	0.9391301847301152
8	0.46672445570655935	0.7719125177081072
9	0.3511290869154192	0.6533213569235377
>10	3.827374792444254	19.977068168800663
>50	2.3237048269063667	33.835490632047176
>100	0.36979291771552525	11.71590248754115
>500	0.010359606708230305	1.3316784679006661
>1k	0.004954594512631885	1.6972591730945694
>5k	9.0083536593307E-4	1.1191835190335016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	6195	0.5751960959087262	RNA PCR Primer, Index 16 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	5824	0.5407493240633449	RNA PCR Primer, Index 16 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	4366	0.4053762961642452	Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1756	0.16304186350536293	Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1747	0.16220622753067712	RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1613	0.14976453635202186	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1543	0.14326514543779897	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1536	0.14261520634637667	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1285	0.11931024749680601	Illumina PCR Primer Index 7 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1161	0.1077970407344683	RNA PCR Primer, Index 16 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	1138	0.10566152657693793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.284844163175565E-5	0.0	0.0	0.5558836200493211	0.0
2	9.284844163175565E-5	0.0	0.0	1.8333853284606472	0.0
3	9.284844163175565E-5	0.0	0.0	2.8098723891018214	0.0
4	9.284844163175565E-5	0.0	0.0	3.3773620643551117	0.0
5	9.284844163175565E-5	0.0	0.0	6.172193005912589	0.0
6	9.284844163175565E-5	0.0	0.0	6.621300918085391	0.0
7	9.284844163175565E-5	0.0	0.0	8.283659417060344	0.0
8	9.284844163175565E-5	0.0	0.0	10.231062631844788	0.0
9	9.284844163175565E-5	0.0	0.0	11.677734200909171	0.0
10	9.284844163175565E-5	0.0	0.0	12.68086876429866	0.0
11	1.856968832635113E-4	0.0	0.0	13.69895192679086	0.0
12	2.7854532489526694E-4	0.0	0.0	14.614066167513444	0.0
13	2.7854532489526694E-4	0.0	0.0	14.997808776777491	0.0
14	2.7854532489526694E-4	0.0	0.0	15.283039189470244	0.0
15	2.7854532489526694E-4	0.0	0.0	16.19824627863446	0.0
16	2.7854532489526694E-4	0.0	0.0	17.17129794693526	0.0
17	2.7854532489526694E-4	0.0	0.0	18.606363460795674	0.0
18	2.7854532489526694E-4	0.0	0.0	19.21340657218409	0.0
19	2.7854532489526694E-4	0.0	0.0	19.88172965504947	0.0
20	3.713937665270226E-4	0.0	0.0	20.72432926285765	0.0
21	3.713937665270226E-4	0.0	0.0	21.384017440651277	0.0
22	3.713937665270226E-4	0.0	0.0	22.1653370769825	0.0
23	4.6424220815877826E-4	0.0	0.0	22.717785304691446	0.0
24	4.6424220815877826E-4	0.0	0.0	23.177942181418427	0.0
25	4.6424220815877826E-4	0.0	0.0	23.638470451911935	0.0
26	4.6424220815877826E-4	0.0	0.0	24.04440383872597	0.0
27	4.6424220815877826E-4	0.0	0.0	24.480605817511957	0.0
28	4.6424220815877826E-4	0.0	0.0	24.88087544938646	0.0
29	4.6424220815877826E-4	0.0	0.0	25.453564637371127	0.0
30	4.6424220815877826E-4	0.0	0.0	25.9984921413079	0.0
31	4.6424220815877826E-4	0.0	0.0	26.442864782957482	0.0
32	4.6424220815877826E-4	0.0	0.0	26.886216091749116	0.0
33	4.6424220815877826E-4	0.0	0.0	27.28212184686692	0.0
34	4.6424220815877826E-4	0.0	0.0	27.9092202216478	0.0
35	4.6424220815877826E-4	0.0	0.0	28.340408384585672	0.0
36	4.6424220815877826E-4	0.0	0.0	28.794344415723327	0.0
37	4.6424220815877826E-4	0.0	0.0	29.230175000742786	0.0
38	4.6424220815877826E-4	0.0	0.0	29.63592269067356	0.0
39	4.6424220815877826E-4	0.0	0.0	30.154852630953442	0.0
40	4.6424220815877826E-4	0.0	0.0	30.628751077041922	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	25	3.4170123E-5	46.0	12
TCGTTCC	20	6.311276E-4	46.0	13
CGAACAC	20	6.311276E-4	46.0	18
ACACGAA	20	6.311276E-4	46.0	32
TCCGCGT	25	3.4170123E-5	46.0	43
ACGCCCG	20	6.311276E-4	46.0	44
GATCGAC	25	3.4170123E-5	46.0	8
CGGTTTA	20	6.311276E-4	46.0	29
GGCCGTA	25	3.4170123E-5	46.0	16
ACCGAGT	25	3.4170123E-5	46.0	13
GTTAGTC	25	3.4170123E-5	46.0	29
TTACTCG	30	1.8612936E-6	46.0	38
CGATTCC	20	6.311276E-4	46.0	39
ACTTACC	20	6.311276E-4	46.0	4
ATCCGTG	20	6.311276E-4	46.0	46
TGTTGCG	20	6.311276E-4	46.0	32
ACGATAT	20	6.311276E-4	46.0	28
CTAACGG	100	0.0	46.0	1
TCTACGG	20	6.311276E-4	46.0	1
TACGATA	20	6.311276E-4	46.0	23
>>END_MODULE