FastQCFastQC Report
Thu 26 May 2016
SRR1544843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544843_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences657192
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT52370.7968751902031674RNA PCR Primer, Index 16 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT41370.6294964028776978RNA PCR Primer, Index 16 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT30390.4624219406200928Illumina PCR Primer Index 7 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTTG21920.3335402743794812Illumina PCR Primer Index 7 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA19530.2971734287696746No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC12730.19370290569574797RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT11820.1798561151079137Illumina PCR Primer Index 7 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC10460.15916201049312834Illumina PCR Primer Index 7 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC9130.13892439348013974Illumina PCR Primer Index 7 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG8940.1360333053354271No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8850.13466384253003688No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT7710.11731731366176094Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC7560.11503487565277727RNA PCR Primer, Index 16 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT7210.1097091869651487No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.10560079854897808No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG6750.10270971040426544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.01867045E-746.0000045
GGATCGA351.01867045E-746.0000047
TGATCGC206.309472E-446.033
CTTCGAC253.4155484E-546.013
AATCCCC301.8601786E-646.036
AGATCCC206.309472E-446.012
GTCGATG301.8601786E-646.042
TTTACGG600.046.01
CTATCTA301.8601786E-646.037
TATATCG206.309472E-446.020
TAAGTCG206.309472E-446.011
TCGATGG501.6370905E-1146.01
TTATCGG206.309472E-446.01
ACGGGCC301.8601786E-646.04
ATCCGCG206.309472E-446.021
GTTACGG453.092282E-1046.01
ATTCCCG206.309472E-446.011
CTAACGG650.046.01
AAGTTGG600.046.01
CTTGCGG1350.046.01
Produced by FastQC (version 0.11.5)