##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544843_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	657192
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88958630050274	34.0	33.0	34.0	31.0	34.0
2	33.24206015897941	34.0	33.0	34.0	31.0	34.0
3	33.338293831939524	34.0	34.0	34.0	31.0	34.0
4	36.580382901800384	37.0	37.0	37.0	35.0	37.0
5	36.57399511862591	37.0	37.0	37.0	35.0	37.0
6	36.49366090883638	37.0	37.0	37.0	35.0	37.0
7	36.37042142935398	37.0	37.0	37.0	35.0	37.0
8	36.42070506031723	37.0	37.0	37.0	35.0	37.0
9	38.28224476256558	39.0	39.0	39.0	37.0	39.0
10	38.10475933973633	39.0	39.0	39.0	35.0	39.0
11	37.27302523463463	39.0	35.0	39.0	35.0	39.0
12	37.1993740033354	39.0	35.0	39.0	35.0	39.0
13	37.1741439335841	39.0	35.0	39.0	35.0	39.0
14	38.34919932074645	40.0	35.0	41.0	35.0	41.0
15	38.42166672753168	40.0	35.0	41.0	35.0	41.0
16	38.42836796552606	40.0	35.0	41.0	35.0	41.0
17	38.52549787581103	40.0	36.0	41.0	35.0	41.0
18	38.52220812182741	40.0	36.0	41.0	35.0	41.0
19	38.438894569623486	40.0	35.0	41.0	35.0	41.0
20	38.35359833960243	40.0	35.0	41.0	35.0	41.0
21	38.291281695455815	40.0	35.0	41.0	35.0	41.0
22	38.28265407978186	40.0	35.0	41.0	35.0	41.0
23	38.26383613921046	40.0	35.0	41.0	35.0	41.0
24	38.19261190032746	40.0	35.0	41.0	35.0	41.0
25	38.125681688152014	40.0	35.0	41.0	35.0	41.0
26	38.050009738402174	40.0	35.0	41.0	35.0	41.0
27	38.08082265152345	40.0	35.0	41.0	35.0	41.0
28	38.12688985867144	40.0	35.0	41.0	35.0	41.0
29	38.15047200818026	40.0	35.0	41.0	35.0	41.0
30	38.08659265481016	40.0	35.0	41.0	35.0	41.0
31	38.00823960121243	40.0	35.0	41.0	35.0	41.0
32	37.994748870954	40.0	35.0	41.0	35.0	41.0
33	37.89986031479385	40.0	35.0	41.0	35.0	41.0
34	37.8536668127427	40.0	35.0	41.0	35.0	41.0
35	37.781275487224434	40.0	35.0	41.0	35.0	41.0
36	37.72654414539434	40.0	35.0	41.0	35.0	41.0
37	37.67562143178858	40.0	35.0	41.0	35.0	41.0
38	37.65077481162278	40.0	35.0	41.0	35.0	41.0
39	37.60049726716089	40.0	35.0	41.0	35.0	41.0
40	37.560726545667016	39.0	35.0	41.0	35.0	41.0
41	37.54139581735627	39.0	35.0	41.0	35.0	41.0
42	37.47243575697817	39.0	35.0	41.0	35.0	41.0
43	37.401220647847204	39.0	35.0	41.0	35.0	41.0
44	37.2594690744866	39.0	35.0	41.0	35.0	41.0
45	37.25024954655565	39.0	35.0	41.0	35.0	41.0
46	37.202537462415854	38.0	35.0	41.0	35.0	41.0
47	37.15960784671755	38.0	35.0	41.0	35.0	41.0
48	37.12760654420626	38.0	35.0	41.0	34.0	41.0
49	37.084836699168584	38.0	35.0	41.0	34.0	41.0
50	37.00933821470742	37.0	35.0	41.0	34.0	41.0
51	36.951251689004124	37.0	35.0	41.0	34.0	41.0
52	36.53313339176375	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	2.0
14	4.0
15	9.0
16	15.0
17	30.0
18	47.0
19	83.0
20	174.0
21	240.0
22	406.0
23	652.0
24	1165.0
25	1736.0
26	2406.0
27	2849.0
28	2872.0
29	3243.0
30	4019.0
31	4982.0
32	6765.0
33	10218.0
34	34249.0
35	161431.0
36	23184.0
37	35344.0
38	63904.0
39	294895.0
40	2263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.967978916359296	21.914295974388	23.71818281415477	22.399542295097934
2	26.610640421672816	24.205407247805816	26.875403230714923	22.30854909980645
3	27.441143531875007	24.530882907886888	25.43579349718195	22.592180063056155
4	25.658863771926622	27.029696040122218	23.432573737963942	23.878866449987218
5	24.23736137993159	33.94989592082679	20.93680385640726	20.875938842834362
6	84.41505678705767	3.2354319590013265	8.307009214963056	4.042502038977955
7	85.99572118954583	2.680190872682565	6.265444497194123	5.058643440577487
8	75.70162144396159	4.801793083299858	11.99436389974315	7.502221572995412
9	53.5840363242401	18.41075363059806	13.77207878367357	14.233131261488271
10	45.9681493383973	18.169728176849382	24.023420857222852	11.838701627530462
11	41.89932318104907	17.4171322840205	22.62854690866596	18.05499762626447
12	20.277179271810976	36.041522112259436	25.897150300064514	17.784148315865075
13	13.551747434539676	38.682150726119616	29.06258749345701	18.703514345883697
14	13.358501016445725	23.293649344483804	44.73517632594432	18.612673313126148
15	15.03350618997188	17.82173246174634	46.97713910090202	20.16762224737976
16	15.105783393589697	20.978952878306494	27.03395659107232	36.88130713703149
17	19.06003116288695	23.349797319504802	32.48365774385567	25.10651377375257
18	24.739193416840134	26.665418933888425	26.55647055959294	22.038917089678513
19	28.335402743794813	22.28146416876651	24.06541771658815	25.31771537085053
20	19.848233088655977	30.57295280526848	27.611413407345193	21.967400698730355
21	20.908197300027997	23.837934728359443	26.071224238883005	29.182643732729552
22	19.348987814824284	29.675498180136096	22.648023713009287	28.327490292030333
23	19.337727787313298	21.972726387417985	37.32242632290107	21.36711950236765
24	19.309425556001898	22.493274416000194	33.12137092356572	25.07592910443219
25	16.40996847192297	32.22482927363693	27.66497461928934	23.70022763515076
26	17.353680507370754	30.70274744671268	26.451782736247548	25.491789309669016
27	19.87729613263704	27.023913863832792	34.740228122070874	18.3585618814593
28	16.80528673507894	23.994509975775724	32.86756381696673	26.332639472178602
29	18.831939524522515	28.78793411970931	27.941149618376365	24.438976737391812
30	29.578114158419456	23.992379700300674	20.722863333698523	25.706642807581346
31	27.542027291872085	26.172564486481882	23.752571546823457	22.53283667482258
32	24.876748347514884	26.93596391921995	21.906840010225324	26.280447723039842
33	17.83725304020743	24.346309754227075	26.819103093159992	30.997334112405504
34	18.390972501186866	25.447814337362594	29.268767726935202	26.892445434515334
35	27.15994716916822	21.020341087536064	24.09843698645145	27.721274756844274
36	21.1130080707008	27.067432348537412	24.343722991150226	27.475836589611557
37	20.045435732632168	29.338914655077968	25.252589806327524	25.363059805962333
38	18.823266260088374	27.228115984369865	23.90686435623075	30.041753399311006
39	24.861379931587724	21.65790210471217	24.293509354952587	29.18720860874752
40	16.689338884222572	20.663063457863153	28.592861751208172	34.05473590670611
41	20.776424545642673	26.041704707300152	22.126867034291347	31.055003712765828
42	21.086988277398387	21.387813606982434	27.0791488636502	30.446049251968983
43	22.439865366589977	24.85498910516257	26.851970200489355	25.8531753277581
44	17.759041497766255	29.791293868458535	24.4502367649028	27.999427868872417
45	21.01349377350911	26.66268000827764	25.61321501174695	26.7106112064663
46	22.53983615138346	22.13036677257179	27.162990419846867	28.166806656197885
47	20.811878416048888	18.4840959719534	32.737464850454664	27.966560761543054
48	19.75602259309304	17.998393163641676	29.70045283570098	32.545131407564305
49	19.039337058272164	24.532708858294075	30.260563123105577	26.167390960328184
50	18.37134353430961	25.761421319796955	27.851678048424205	28.015557097469234
51	19.741414989835544	20.960236886632828	28.692528210933794	30.60581991259784
52	22.874137238432603	19.298013365956983	32.3176484193356	25.510200976274817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	91.0
1	151.0
2	211.0
3	855.5
4	1500.0
5	1057.0
6	614.0
7	693.5
8	773.0
9	784.5
10	796.0
11	791.0
12	786.0
13	791.5
14	808.5
15	820.0
16	745.0
17	670.0
18	742.5
19	815.0
20	751.5
21	688.0
22	893.0
23	1098.0
24	1274.0
25	1450.0
26	1801.5
27	2153.0
28	2809.5
29	3466.0
30	3821.0
31	4176.0
32	5032.5
33	5889.0
34	6770.5
35	7652.0
36	8808.0
37	9964.0
38	11032.5
39	13751.5
40	15402.0
41	22918.0
42	30434.0
43	41824.0
44	53214.0
45	56666.5
46	60119.0
47	59779.5
48	59440.0
49	67707.5
50	75975.0
51	78593.0
52	81211.0
53	69756.5
54	58302.0
55	50030.0
56	41758.0
57	35111.0
58	28464.0
59	25896.0
60	23328.0
61	22406.0
62	21484.0
63	20605.0
64	15542.0
65	11358.0
66	9377.0
67	7396.0
68	6303.5
69	5211.0
70	4123.0
71	3035.0
72	2583.5
73	2132.0
74	1812.0
75	1492.0
76	1045.0
77	598.0
78	413.0
79	228.0
80	255.0
81	282.0
82	177.5
83	73.0
84	45.0
85	17.0
86	9.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	657192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.616939335718705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.22949922197205	17.2883532192519
2	12.11047084516523	5.962454522448449
3	4.631235202965185	3.420205081225166
4	2.195483782297613	2.1618436432549837
5	1.1998947038341587	1.4768867566767823
6	0.8188021821578257	1.2093842187679975
7	0.5393371601780156	0.9293781107520707
8	0.4602677388360246	0.9064306404091871
9	0.3513108805768598	0.7783378771624627
>10	6.546772633275044	43.70194235709686
>50	0.761724963443777	12.008344127058228
>100	0.14278463047438364	5.8000774355630895
>500	0.007449632894315668	1.2908927910363193
>1k	0.004345619188350806	2.265137084022768
>5k	6.208027411929723E-4	0.8003321352737307
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	5237	0.7968751902031674	RNA PCR Primer, Index 16 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	4137	0.6294964028776978	RNA PCR Primer, Index 16 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	3039	0.4624219406200928	Illumina PCR Primer Index 7 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTTG	2192	0.3335402743794812	Illumina PCR Primer Index 7 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1953	0.2971734287696746	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1273	0.19370290569574797	RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1182	0.1798561151079137	Illumina PCR Primer Index 7 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1046	0.15916201049312834	Illumina PCR Primer Index 7 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	913	0.13892439348013974	Illumina PCR Primer Index 7 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	894	0.1360333053354271	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	885	0.13466384253003688	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	771	0.11731731366176094	Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	756	0.11503487565277727	RNA PCR Primer, Index 16 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	721	0.1097091869651487	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694	0.10560079854897808	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	675	0.10270971040426544	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6427345433298032	0.0
2	0.0	0.0	0.0	2.2436365628309534	0.0
3	0.0	0.0	0.0	3.3559446858756647	0.0
4	0.0	0.0	0.0	4.039915275901106	0.0
5	0.0	0.0	0.0	7.330430072185906	0.0
6	1.5216253393224505E-4	0.0	0.0	7.873345993256157	0.0
7	1.5216253393224505E-4	0.0	0.0	9.753466262522977	0.0
8	1.5216253393224505E-4	0.0	0.0	11.895306090153259	0.0
9	1.5216253393224505E-4	0.0	0.0	13.569702613543683	0.0
10	1.5216253393224505E-4	0.0	0.0	14.738006549075461	0.0
11	3.043250678644901E-4	0.0	0.0	15.87329121474394	0.0
12	3.043250678644901E-4	0.0	0.0	16.917887010188803	0.0
13	3.043250678644901E-4	0.0	0.0	17.369505410899706	0.0
14	3.043250678644901E-4	0.0	0.0	17.67930832998576	0.0
15	3.043250678644901E-4	0.0	0.0	18.65390935982179	0.0
16	3.043250678644901E-4	0.0	0.0	19.672941849566033	0.0
17	3.043250678644901E-4	0.0	0.0	21.09064017821276	0.0
18	3.043250678644901E-4	0.0	0.0	21.71541954253856	0.0
19	3.043250678644901E-4	0.0	0.0	22.413236923151835	0.0
20	3.043250678644901E-4	0.0	0.0	23.285584730185395	0.0
21	3.043250678644901E-4	0.0	0.0	24.00150945233661	0.0
22	3.043250678644901E-4	0.0	0.0	24.82501308597792	0.0
23	3.043250678644901E-4	0.0	0.0	25.397448538631025	0.0
24	3.043250678644901E-4	0.0	0.0	25.882694859340955	0.0
25	3.043250678644901E-4	0.0	0.0	26.374788494077833	0.0
26	3.043250678644901E-4	0.0	0.0	26.803734677232832	0.0
27	3.043250678644901E-4	0.0	0.0	27.250331714323973	0.0
28	3.043250678644901E-4	0.0	0.0	27.64930187829432	0.0
29	3.043250678644901E-4	0.0	0.0	28.22356328135461	0.0
30	3.043250678644901E-4	0.0	0.0	28.759936213465775	0.0
31	3.043250678644901E-4	0.0	0.0	29.203946487480067	0.0
32	3.043250678644901E-4	0.0	0.0	29.657847326199953	0.0
33	3.043250678644901E-4	0.0	0.0	30.06549075460444	0.0
34	3.043250678644901E-4	0.0	0.0	30.67353224019769	0.0
35	3.043250678644901E-4	0.0	0.0	31.07143726643051	0.0
36	3.043250678644901E-4	0.0	0.0	31.525946755286128	0.0
37	3.043250678644901E-4	0.0	0.0	31.959457814459093	0.0
38	3.043250678644901E-4	0.0	0.0	32.362232041777744	0.0
39	3.043250678644901E-4	0.0	0.0	32.88521467090287	0.0
40	3.043250678644901E-4	0.0	0.0	33.34535417351398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.01867045E-7	46.000004	5
GGATCGA	35	1.01867045E-7	46.000004	7
TGATCGC	20	6.309472E-4	46.0	33
CTTCGAC	25	3.4155484E-5	46.0	13
AATCCCC	30	1.8601786E-6	46.0	36
AGATCCC	20	6.309472E-4	46.0	12
GTCGATG	30	1.8601786E-6	46.0	42
TTTACGG	60	0.0	46.0	1
CTATCTA	30	1.8601786E-6	46.0	37
TATATCG	20	6.309472E-4	46.0	20
TAAGTCG	20	6.309472E-4	46.0	11
TCGATGG	50	1.6370905E-11	46.0	1
TTATCGG	20	6.309472E-4	46.0	1
ACGGGCC	30	1.8601786E-6	46.0	4
ATCCGCG	20	6.309472E-4	46.0	21
GTTACGG	45	3.092282E-10	46.0	1
ATTCCCG	20	6.309472E-4	46.0	11
CTAACGG	65	0.0	46.0	1
AAGTTGG	60	0.0	46.0	1
CTTGCGG	135	0.0	46.0	1
>>END_MODULE