FastQCFastQC Report
Thu 26 May 2016
SRR1544842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544842_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences572708
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT31930.5575266977237964Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT24730.43180818148166256Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT16460.2874064968535449Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC7850.13706810451399315No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC7700.1344489687589487No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT7630.13322670540659465Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.12659156149381534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG351.0183612E-746.0000041
GACCGGG351.0183612E-746.0000042
ACCGAGG351.0183612E-746.0000041
CAATTGC351.0183612E-746.00000424
ATATGCG351.0183612E-746.00000418
TCGATAT351.0183612E-746.00000415
GTTACGG351.0183612E-746.0000041
TACGCGG1100.046.0000041
GCTTAGG551.8189894E-1246.0000041
GCGGGTA351.0183612E-746.0000044
CGGGACC551.8189894E-1246.0000045
ACGTAGG351.0183612E-746.0000041
TCATAGG1100.046.0000041
TCGCCTA206.3087884E-446.09
CTTCGAC206.3087884E-446.036
CGGGTAA253.414994E-546.05
ATCTCGA206.3087884E-446.044
TTAATAC405.6024874E-946.046
GTCGAGG501.6370905E-1146.01
GATCGCC206.3087884E-446.020
Produced by FastQC (version 0.11.5)