##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544842_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	572708
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9414361245172	34.0	33.0	34.0	31.0	34.0
2	33.237567835616055	34.0	33.0	34.0	31.0	34.0
3	33.2799611669472	34.0	34.0	34.0	31.0	34.0
4	36.565309023097285	37.0	37.0	37.0	35.0	37.0
5	36.562310985703014	37.0	37.0	37.0	35.0	37.0
6	36.687345034467825	37.0	37.0	37.0	35.0	37.0
7	36.67115004504913	37.0	37.0	37.0	35.0	37.0
8	36.585038099694785	37.0	37.0	37.0	35.0	37.0
9	38.37850178450449	39.0	39.0	39.0	37.0	39.0
10	38.05766289278306	39.0	39.0	39.0	35.0	39.0
11	36.89421136076325	39.0	35.0	39.0	35.0	39.0
12	36.72804815019172	39.0	35.0	39.0	35.0	39.0
13	36.70054024040174	39.0	35.0	39.0	35.0	39.0
14	37.80155332211179	40.0	35.0	41.0	35.0	41.0
15	37.892219071498914	40.0	35.0	41.0	35.0	41.0
16	37.90585603833018	40.0	35.0	41.0	35.0	41.0
17	38.02852064228193	40.0	35.0	41.0	35.0	41.0
18	38.01689691780104	39.0	35.0	41.0	35.0	41.0
19	37.87009610482131	39.0	35.0	41.0	35.0	41.0
20	37.77582642463524	39.0	35.0	41.0	35.0	41.0
21	37.711830461596485	39.0	35.0	41.0	35.0	41.0
22	37.69520768000447	39.0	35.0	41.0	35.0	41.0
23	37.67260628452894	39.0	35.0	41.0	35.0	41.0
24	37.63477199550207	38.0	35.0	41.0	35.0	41.0
25	37.592282629193235	38.0	35.0	41.0	35.0	41.0
26	37.549354994168056	38.0	35.0	41.0	35.0	41.0
27	37.57529491468602	38.0	35.0	41.0	35.0	41.0
28	37.62220538214937	38.0	35.0	41.0	35.0	41.0
29	37.61140406629556	38.0	35.0	41.0	35.0	41.0
30	37.47687477737346	38.0	35.0	41.0	35.0	41.0
31	37.324767595354004	38.0	35.0	41.0	35.0	41.0
32	37.236710505178905	38.0	35.0	41.0	35.0	41.0
33	37.166561319206295	38.0	35.0	41.0	35.0	41.0
34	36.938504438562056	37.0	35.0	41.0	34.0	41.0
35	36.968275281644395	37.0	35.0	41.0	34.0	41.0
36	36.81450058319423	37.0	35.0	41.0	34.0	41.0
37	36.71486167471032	36.0	35.0	41.0	34.0	41.0
38	36.68495812874973	36.0	35.0	41.0	34.0	41.0
39	36.62750476682707	36.0	35.0	41.0	33.0	41.0
40	36.62909720136614	36.0	35.0	41.0	34.0	41.0
41	36.61641185385921	36.0	35.0	41.0	34.0	41.0
42	36.55346005294147	36.0	35.0	40.0	34.0	41.0
43	36.60085593356475	36.0	35.0	41.0	34.0	41.0
44	36.53069103277761	35.0	35.0	41.0	34.0	41.0
45	36.52103340620351	35.0	35.0	41.0	34.0	41.0
46	36.43904048834659	35.0	35.0	41.0	34.0	41.0
47	36.40279339558728	35.0	35.0	40.0	34.0	41.0
48	36.42899872186175	36.0	35.0	40.0	34.0	41.0
49	36.35035655168079	35.0	35.0	40.0	34.0	41.0
50	36.26319520593391	35.0	35.0	40.0	34.0	41.0
51	36.17572480216795	36.0	35.0	40.0	33.0	41.0
52	35.96324304881371	35.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	4.0
16	6.0
17	14.0
18	42.0
19	71.0
20	144.0
21	271.0
22	460.0
23	709.0
24	1272.0
25	2033.0
26	3000.0
27	3343.0
28	3471.0
29	3657.0
30	4351.0
31	5453.0
32	7206.0
33	11119.0
34	38309.0
35	180990.0
36	17909.0
37	27692.0
38	50619.0
39	209224.0
40	1338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.56653652472115	21.586742284026066	25.763739986170965	22.082981205081822
2	24.71102202169343	25.08905061567151	28.27706265671162	21.922864705923438
3	25.62789414500932	25.339789211954432	26.665071904006926	22.367244739029314
4	25.833758215355818	27.62262793605118	24.784183213784335	21.759430634808663
5	23.873422407230212	35.14461121548852	21.240667146259526	19.741299231021742
6	87.86833779168441	2.3640319325031256	6.9937909021700415	2.773839373642415
7	89.12552295410576	1.6816597637888768	5.120061183011238	4.0727560990941285
8	80.72438310622516	3.469132612081549	10.129245619058928	5.677238662634362
9	62.260349078413434	14.078553119565292	11.50743485336332	12.153662948657955
10	54.01321441292946	15.64462169203154	19.52495861765507	10.817205277383938
11	47.460485971908895	16.371868386682216	20.872940486251284	15.294705155157601
12	17.937063913896786	43.13018152356873	24.064793926398792	14.867960636135692
13	11.327412922466596	45.0648498012949	28.103501260677344	15.50423601556116
14	10.168008828233585	20.891623654637268	54.40678321238747	14.533584304741684
15	11.765157811659693	14.35356237384496	54.74569937909022	19.135580435405128
16	12.191553112580932	17.046208539080997	27.11783317152895	43.64440517680912
17	18.70656599872885	21.05191476284599	36.306983663577256	23.934535574847914
18	26.43808013856974	24.326532892852903	26.139498662494674	23.09588830608268
19	30.565139652318457	22.0443926049575	23.54568121974898	23.84478652297506
20	18.39611110723091	31.51047305083917	27.210201359156848	22.88321448277307
21	20.17101210389937	24.004728413083107	25.119083372329353	30.70517611068817
22	17.035557387010485	31.550633132416518	21.62620392940207	29.787605551170927
23	17.907031157238944	22.17220642980367	40.165843676009416	19.754918736947975
24	15.691766135622343	20.904020897211144	38.163601695803095	25.240611271363417
25	13.423594571753844	34.41753912988818	28.404003436306112	23.754862862051866
26	16.700133401314456	31.02767902665931	28.309539940074174	23.96264763195206
27	16.020031150254578	26.633991493047066	39.965392486223344	17.38058487047501
28	14.729670268269345	22.568743583117403	36.28585596848656	26.415730180126694
29	18.431905962549852	27.03925909887761	32.12143011796588	22.40740482060666
30	30.96359750518589	23.393771345956406	21.024151923842517	24.618479225015193
31	34.110925637497644	23.562094470480595	24.087667711992847	18.239312180028914
32	29.38443325394442	24.899774405106964	24.231894787570628	21.483897553377986
33	21.51375570098549	24.496602107880456	27.203566215244066	26.786075975889982
34	20.174678893956433	22.75871822988329	32.10833443919065	24.958268436969625
35	37.58093129483087	17.931825642386695	24.79343749345216	19.69380556933027
36	25.173386786983944	27.137738603267287	27.853461100595766	19.835413509153007
37	24.29737318144674	28.50999112986024	24.36477227487655	22.827863413816466
38	22.353450624052744	28.888718160039673	26.380633761009097	22.377197454898482
39	28.32298483695007	23.371595996563695	23.51844220789652	24.78697695858972
40	17.563575155227447	20.185155436976608	29.724397074949188	32.52687233284676
41	23.83430997995488	23.415422868198103	23.342960112308543	29.407307039538473
42	24.127653184519858	22.20712823987093	25.793947351879144	27.87127122373007
43	25.055874896107618	27.07400629989454	26.368411127485558	21.50170767651229
44	18.045845352256297	33.08073224051349	23.91305866165655	24.96036374557366
45	24.89348847929486	26.88228556262528	25.954413069138198	22.269812888941658
46	24.708402885938384	22.09852141056175	28.145407432758056	25.04766827074181
47	25.605718795616617	17.871410910970337	31.61715918059465	24.9057111128184
48	21.609790678670457	17.632894948210957	30.96883577669598	29.788478596422607
49	21.402355126870937	25.98881105205445	29.75023921439896	22.858594606675652
50	19.629549438806514	28.847859642260975	25.454681967075715	26.067908951856793
51	21.14375912332288	21.417895332350867	30.17139624381011	27.266949300516146
52	25.35428176313235	18.938097599474776	32.38107377581595	23.32654686157693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	142.5
2	183.0
3	833.5
4	1484.0
5	1043.0
6	602.0
7	758.0
8	914.0
9	973.0
10	1032.0
11	1089.0
12	1146.0
13	1079.0
14	866.5
15	721.0
16	722.5
17	724.0
18	678.5
19	633.0
20	679.0
21	725.0
22	748.5
23	772.0
24	891.5
25	1011.0
26	1172.5
27	1334.0
28	1625.0
29	1916.0
30	2288.5
31	2661.0
32	3013.5
33	3366.0
34	4199.0
35	5032.0
36	5763.5
37	6495.0
38	7123.0
39	10405.0
40	13059.0
41	22597.0
42	32135.0
43	46291.0
44	60447.0
45	62630.0
46	64813.0
47	60378.5
48	55944.0
49	60553.0
50	65162.0
51	65510.5
52	65859.0
53	58452.5
54	51046.0
55	42284.0
56	33522.0
57	28738.0
58	23954.0
59	21543.0
60	19132.0
61	18339.5
62	17547.0
63	14770.0
64	9902.5
65	7812.0
66	6112.5
67	4413.0
68	3455.5
69	2498.0
70	1983.5
71	1469.0
72	1242.5
73	1016.0
74	765.0
75	514.0
76	421.5
77	329.0
78	240.0
79	151.0
80	183.5
81	216.0
82	136.0
83	56.0
84	30.0
85	4.0
86	2.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	572708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.34769952415698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81180296308347	16.299449988217248
2	12.570396043344045	5.869796594392971
3	4.867470724953912	3.4093273178656345
4	2.347238512796941	2.192104780332483
5	1.316433807759242	1.5367850493502306
6	0.7896746922621832	1.106225246206115
7	0.5493898325813712	0.8978892112915733
8	0.4182672286566055	0.781246206038103
9	0.29253022831096676	0.6146917085103741
>10	5.301164364419055	35.427777756374695
>50	1.4977303736500356	22.103727282126567
>100	0.23042005748462085	7.671598741639714
>500	0.005236819488286838	0.8122067837395476
>1k	0.0022443512092657878	1.2771733339147469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	3193	0.5575266977237964	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	2473	0.43180818148166256	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1646	0.2874064968535449	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	785	0.13706810451399315	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	770	0.1344489687589487	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	763	0.13322670540659465	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.12659156149381534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44455464215621227	0.0
2	0.0	0.0	0.0	1.5449408773755562	0.0
3	0.0	0.0	0.0	2.382540491838773	0.0
4	0.0	0.0	0.0	2.899907107985221	0.0
5	0.0	0.0	0.0	5.482724180559726	0.0
6	0.0	0.0	0.0	5.947184254454277	0.0
7	0.0	0.0	0.0	7.910488416435601	0.0
8	0.0	0.0	0.0	10.112832368327314	0.0
9	0.0	0.0	0.0	11.946751224009443	0.0
10	0.0	0.0	0.0	12.950927872493487	0.0
11	0.0	0.0	0.0	13.957549047682239	0.0
12	0.0	0.0	0.0	14.889262940276721	0.0
13	0.0	0.0	0.0	15.2042576670834	0.0
14	0.0	0.0	0.0	15.444868938446817	0.0
15	0.0	0.0	0.0	16.282643161960372	0.0
16	0.0	0.0	0.0	17.21680158125956	0.0
17	0.0	0.0	0.0	18.788108425235897	0.0
18	0.0	0.0	0.0	19.32799960887573	0.0
19	0.0	0.0	0.0	19.995704617361728	0.0
20	0.0	0.0	0.0	20.827542133163846	0.0
21	0.0	0.0	0.0	21.4512456609651	0.0
22	0.0	0.0	0.0	22.255494946814082	0.0
23	0.0	0.0	0.0	22.76867094575246	0.0
24	3.4921810067259406E-4	0.0	0.0	23.18790727560991	0.0
25	3.4921810067259406E-4	0.0	0.0	23.655859530511187	0.0
26	3.4921810067259406E-4	0.0	0.0	24.00455380403277	0.0
27	3.4921810067259406E-4	0.0	0.0	24.3991702577928	0.0
28	3.4921810067259406E-4	0.0	0.0	24.763055518693644	0.0
29	3.4921810067259406E-4	0.0	0.0	25.375584067273376	0.0
30	3.4921810067259406E-4	0.0	0.0	25.94725409807441	0.0
31	3.4921810067259406E-4	0.0	0.0	26.36229981072379	0.0
32	3.4921810067259406E-4	0.0	0.0	26.7693135070577	0.0
33	3.4921810067259406E-4	0.0	0.0	27.165676051321093	0.0
34	3.4921810067259406E-4	0.0	0.0	27.85101657389106	0.0
35	3.4921810067259406E-4	0.0	0.0	28.29312668934256	0.0
36	3.4921810067259406E-4	0.0	0.0	28.73872898580079	0.0
37	3.4921810067259406E-4	0.0	0.0	29.146091900235373	0.0
38	3.4921810067259406E-4	0.0	0.0	29.523422058012113	0.0
39	3.4921810067259406E-4	0.0	0.0	30.009359045098027	0.0
40	3.4921810067259406E-4	0.0	0.0	30.481327308157038	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGG	35	1.0183612E-7	46.000004	1
GACCGGG	35	1.0183612E-7	46.000004	2
ACCGAGG	35	1.0183612E-7	46.000004	1
CAATTGC	35	1.0183612E-7	46.000004	24
ATATGCG	35	1.0183612E-7	46.000004	18
TCGATAT	35	1.0183612E-7	46.000004	15
GTTACGG	35	1.0183612E-7	46.000004	1
TACGCGG	110	0.0	46.000004	1
GCTTAGG	55	1.8189894E-12	46.000004	1
GCGGGTA	35	1.0183612E-7	46.000004	4
CGGGACC	55	1.8189894E-12	46.000004	5
ACGTAGG	35	1.0183612E-7	46.000004	1
TCATAGG	110	0.0	46.000004	1
TCGCCTA	20	6.3087884E-4	46.0	9
CTTCGAC	20	6.3087884E-4	46.0	36
CGGGTAA	25	3.414994E-5	46.0	5
ATCTCGA	20	6.3087884E-4	46.0	44
TTAATAC	40	5.6024874E-9	46.0	46
GTCGAGG	50	1.6370905E-11	46.0	1
GATCGCC	20	6.3087884E-4	46.0	20
>>END_MODULE