FastQCFastQC Report
Thu 26 May 2016
SRR1544841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544841_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences410875
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT31960.7778521448128993Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT24830.6043200486766048Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT17230.41934895041070885Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTTG12760.31055673866747796Illumina Single End Adapter 1 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC6850.16671737146334045No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT6840.16647398843930636Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC6190.15065409187709156No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC5550.13507757833891085No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA5520.13434742926680865No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT4690.11414663827198053Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC4490.10927897779129905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA351.0174335E-746.00000419
GCCGGAT351.0174335E-746.00000418
CGGATAA351.0174335E-746.00000420
CCATAGG351.0174335E-746.0000041
CGAATGT351.0174335E-746.00000410
TTCGGTA351.0174335E-746.00000434
ACGTAGG351.0174335E-746.0000041
ATTGCGG700.046.0000041
AGGGCGC351.0174335E-746.0000045
TCGGTAC351.0174335E-746.00000435
GCCCGCG351.0174335E-746.0000045
GACTAAT351.0174335E-746.0000048
CTAGCGG1400.046.0000041
AGGTACT206.3066976E-446.022
GCCCAAT206.3066976E-446.08
ACGCCGA206.3066976E-446.041
CGTATAA206.3066976E-446.021
CGAAATG253.4132965E-546.045
ATAGGGT206.3066976E-446.03
TTTACGG253.4132965E-546.01
Produced by FastQC (version 0.11.5)