##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544841_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	410875
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97421600243383	34.0	33.0	34.0	31.0	34.0
2	33.28465591724977	34.0	34.0	34.0	31.0	34.0
3	33.364534225737756	34.0	34.0	34.0	31.0	34.0
4	36.605622147855186	37.0	37.0	37.0	35.0	37.0
5	36.5955533921509	37.0	37.0	37.0	35.0	37.0
6	36.56241192576818	37.0	37.0	37.0	35.0	37.0
7	36.45557894736842	37.0	37.0	37.0	35.0	37.0
8	36.49248554913295	37.0	37.0	37.0	35.0	37.0
9	38.339339215089744	39.0	39.0	39.0	37.0	39.0
10	38.100760571950104	39.0	39.0	39.0	35.0	39.0
11	37.07367934286584	39.0	35.0	39.0	35.0	39.0
12	36.95990264679039	39.0	35.0	39.0	35.0	39.0
13	36.938560389412835	39.0	35.0	39.0	35.0	39.0
14	38.051417097657435	40.0	35.0	41.0	35.0	41.0
15	38.13043626407058	40.0	35.0	41.0	35.0	41.0
16	38.154596896866444	40.0	35.0	41.0	35.0	41.0
17	38.2792941892303	40.0	36.0	41.0	35.0	41.0
18	38.27582841496805	40.0	36.0	41.0	35.0	41.0
19	38.14663583815029	40.0	35.0	41.0	35.0	41.0
20	38.06847337998175	40.0	35.0	41.0	35.0	41.0
21	38.0073964101004	40.0	35.0	41.0	35.0	41.0
22	37.994380285975055	40.0	35.0	41.0	35.0	41.0
23	37.98778217219349	40.0	35.0	41.0	35.0	41.0
24	37.93508244599939	40.0	35.0	41.0	35.0	41.0
25	37.88988865226651	40.0	35.0	41.0	35.0	41.0
26	37.840567082446	40.0	35.0	41.0	35.0	41.0
27	37.87884879829632	39.0	35.0	41.0	35.0	41.0
28	37.936048676604806	39.0	35.0	41.0	35.0	41.0
29	37.92465591724977	40.0	35.0	41.0	35.0	41.0
30	37.82972680255552	39.0	35.0	41.0	35.0	41.0
31	37.70808396714329	39.0	35.0	41.0	35.0	41.0
32	37.68029936111956	39.0	35.0	41.0	35.0	41.0
33	37.65135625190143	39.0	35.0	41.0	35.0	41.0
34	37.43203894128384	38.0	35.0	41.0	35.0	41.0
35	37.46634621235169	39.0	35.0	41.0	35.0	41.0
36	37.35951566778217	38.0	35.0	41.0	35.0	41.0
37	37.2741174323091	38.0	35.0	41.0	35.0	41.0
38	37.251093398235476	38.0	35.0	41.0	35.0	41.0
39	37.24950167325829	38.0	35.0	41.0	35.0	41.0
40	37.27449710982659	38.0	35.0	41.0	35.0	41.0
41	37.26281472467296	38.0	35.0	41.0	35.0	41.0
42	37.228096136294496	38.0	35.0	41.0	35.0	41.0
43	37.18706419227259	38.0	35.0	41.0	35.0	41.0
44	37.03367934286584	37.0	35.0	41.0	35.0	41.0
45	37.020015819896564	37.0	35.0	41.0	35.0	41.0
46	36.9334298752662	37.0	35.0	41.0	34.0	41.0
47	36.91327532704594	37.0	35.0	41.0	34.0	41.0
48	36.91625676909035	37.0	35.0	41.0	34.0	41.0
49	36.84461940979617	36.0	35.0	41.0	34.0	41.0
50	36.75952296927289	36.0	35.0	41.0	34.0	41.0
51	36.70464983267417	36.0	35.0	40.0	34.0	41.0
52	36.31572376026772	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	1.0
15	9.0
16	4.0
17	12.0
18	29.0
19	53.0
20	71.0
21	162.0
22	239.0
23	404.0
24	697.0
25	1119.0
26	1557.0
27	1759.0
28	1862.0
29	1924.0
30	2365.0
31	2955.0
32	4111.0
33	6385.0
34	22712.0
35	123123.0
36	11741.0
37	19223.0
38	36218.0
39	171440.0
40	698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.377365378764832	21.48634012777609	24.981320352905385	23.154974140553698
2	24.290599330696683	25.125159720109526	28.14237906905993	22.44186188013386
3	25.829510191664127	25.302099178582292	26.54505628232431	22.323334347429267
4	26.068025555217524	27.108974749011256	24.655673866747794	22.167325829023426
5	23.675813811986615	34.8385762093094	21.279951323395192	20.205658655308792
6	85.76477030727106	2.4783693337389714	8.00486766048068	3.751992698509279
7	87.08682689382415	1.7796166717371462	5.935138424094919	5.1984180103437785
8	77.25196227563127	3.7227867356251902	11.983206571341649	7.042044417401887
9	59.25524794645574	14.323821113477337	12.586066321874048	13.83486461819288
10	50.85682993611196	15.687496197140248	22.062671128688773	11.39300273805902
11	44.58509278977792	16.36994219653179	22.0057195010648	17.039245512625495
12	17.44837237602677	40.35339215089748	26.087496197140247	16.110739275935504
13	11.284940675387892	42.69230301186492	29.208883480377246	16.813872832369942
14	10.204806814724673	21.229084271372074	52.29960450258595	16.26650441131731
15	11.540249467599635	15.124307879525404	53.43474292668087	19.900699726194098
16	11.84764222695467	18.492728932156982	27.742987526620016	41.91664131426833
17	17.59756616975966	21.36270155156678	36.2971706723456	24.74256160632796
18	25.274353513842406	25.148767873440825	26.787709157286276	22.789169455430482
19	29.05579555825981	22.23815028901734	23.72156982050502	24.984484332217825
20	17.54791603285671	32.68171585031944	27.033769394584727	22.736598722239123
21	19.420991785822938	24.349011256464863	25.168968664435653	31.061028293276543
22	16.650806206267113	31.92065713416489	21.597079403711593	29.831457255856403
23	16.836507453605112	22.643626407058107	39.653665956799514	20.866200182537266
24	16.38892607240645	21.201338606632188	37.19987830848798	25.20985701247338
25	13.316945543048373	33.72169151201704	28.461454213568604	24.499908731365988
26	16.986188013386066	29.54329175540006	27.89899604502586	25.571524186188014
27	17.749680559780955	25.769151201703682	38.51682385153635	17.964344386979008
28	15.972254335260116	22.58521448128993	34.50441131731062	26.93811986613934
29	19.491572862792818	26.102099178582293	31.23650745360511	23.169820505019775
30	30.888956495284454	22.67770003042288	20.23827198052936	26.19507149376331
31	34.51682385153635	22.596653483419534	23.737389717067234	19.14913294797688
32	29.6321265591725	23.427806510495895	24.52400365074536	22.416063279586247
33	22.51828414968056	22.967569212047458	27.224825068451473	27.28932156982051
34	21.539397627015518	21.191359902646788	32.39038637055066	24.87885609978704
35	38.22792820200791	17.356860358989962	24.291329479768788	20.123881959233344
36	26.14371767569212	26.854882871919685	26.970246425311835	20.03115302707636
37	25.280438089443262	27.509826589595377	25.372680255552176	21.83705506540919
38	23.14621235168847	28.364344386979006	25.600730149072103	22.88871311226042
39	29.511165196227562	22.49126863401278	23.79920900517189	24.19835716458777
40	18.017158503194402	19.659993915424398	29.862366899908732	32.46048068147247
41	23.727167630057803	23.17371463340432	23.42464253118345	29.67447520535443
42	23.809674475205355	21.080377243687252	26.65019774870703	28.459750532400363
43	26.283176148463642	25.67423182233039	26.463766352296926	21.578825676909037
44	18.517310617584425	30.807179799209006	25.262184362640706	25.413325220565863
45	24.459263766352297	25.616306662610285	26.3350167325829	23.589412838454518
46	25.324490416793427	20.38113781563736	28.62257377547916	25.67179799209005
47	24.639123821113476	16.950897474901126	32.78515363553392	25.62482506845147
48	21.558624885914206	17.07964709461515	30.54773349558868	30.81399452388196
49	21.546699117736537	24.192272588986917	30.817888652266507	23.44313964101004
50	20.122421661089138	26.642896257986003	26.887739580164283	26.346942500760573
51	20.932887131122605	19.735199269850927	30.73051414663827	28.6013994523882
52	24.92193489504107	17.943413446912075	34.00352905384849	23.131122604198357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	77.0
2	89.0
3	411.5
4	734.0
5	561.0
6	388.0
7	447.0
8	506.0
9	529.0
10	552.0
11	567.5
12	583.0
13	574.0
14	514.0
15	463.0
16	437.5
17	412.0
18	365.0
19	318.0
20	425.5
21	533.0
22	516.5
23	500.0
24	571.5
25	643.0
26	754.0
27	865.0
28	1015.0
29	1165.0
30	1440.5
31	1716.0
32	1965.0
33	2214.0
34	2732.0
35	3250.0
36	3765.0
37	4280.0
38	4782.5
39	6842.0
40	8399.0
41	14647.0
42	20895.0
43	30314.5
44	39734.0
45	41502.5
46	43271.0
47	41546.5
48	39822.0
49	45824.5
50	51827.0
51	52599.0
52	53371.0
53	46522.0
54	39673.0
55	32556.0
56	25439.0
57	21344.0
58	17249.0
59	15278.5
60	13308.0
61	12514.0
62	11720.0
63	10215.5
64	6911.0
65	5111.0
66	4088.5
67	3066.0
68	2365.5
69	1665.0
70	1307.0
71	949.0
72	784.5
73	620.0
74	504.0
75	388.0
76	308.5
77	229.0
78	175.0
79	121.0
80	133.5
81	146.0
82	90.0
83	34.0
84	17.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	410875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.017628727189628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.60216141154345	16.912457753495396
2	13.215546676202344	6.612432803441504
3	4.864987204826305	3.6513133095861767
4	2.3145129314385318	2.3161450081203396
5	1.1914315681960477	1.4903396313491017
6	0.8102312770423636	1.2162039163321547
7	0.5747433802394598	1.0065101550168547
8	0.47296570441781965	0.946598431505498
9	0.3891489764329093	0.8762026150767953
>10	7.949615055203044	49.14997372110509
>50	0.4869351257392355	8.223822999721806
>100	0.1187403277929693	4.659101276918643
>500	0.004989089403065937	0.7726060036467639
>1k	0.00399127152245275	2.166292374683883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	3196	0.7778521448128993	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	2483	0.6043200486766048	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1723	0.41934895041070885	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTTG	1276	0.31055673866747796	Illumina Single End Adapter 1 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	685	0.16671737146334045	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	684	0.16647398843930636	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	619	0.15065409187709156	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	555	0.13507757833891085	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	552	0.13434742926680865	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	469	0.11414663827198053	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	449	0.10927897779129905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6167325829023426	0.0
2	0.0	0.0	0.0	2.1242470337693944	0.0
3	0.0	0.0	0.0	3.209005171889261	0.0
4	0.0	0.0	0.0	3.91335564344387	0.0
5	0.0	0.0	0.0	7.327045938545786	0.0
6	0.0	0.0	0.0	7.9104350471554605	0.0
7	0.0	0.0	0.0	10.457195010648007	0.0
8	0.0	0.0	0.0	13.239306358381503	0.0
9	0.0	0.0	0.0	15.623729844843322	0.0
10	0.0	0.0	0.0	16.915120170368116	0.0
11	0.0	0.0	0.0	18.12205658655309	0.0
12	0.0	0.0	0.0	19.301003954974142	0.0
13	0.0	0.0	0.0	19.70721022208701	0.0
14	0.0	0.0	0.0	20.01679342865835	0.0
15	0.0	0.0	0.0	20.983510800121692	0.0
16	0.0	0.0	0.0	22.04758138119866	0.0
17	2.4338302403407362E-4	0.0	0.0	23.7807118953453	0.0
18	2.4338302403407362E-4	0.0	0.0	24.395984180103437	0.0
19	2.4338302403407362E-4	0.0	0.0	25.135381807118954	0.0
20	2.4338302403407362E-4	0.0	0.0	26.051962275631276	0.0
21	2.4338302403407362E-4	0.0	0.0	26.748767873440826	0.0
22	2.4338302403407362E-4	0.0	0.0	27.64393063583815	0.0
23	2.4338302403407362E-4	0.0	0.0	28.214907210222087	0.0
24	2.4338302403407362E-4	0.0	0.0	28.677334955886828	0.0
25	2.4338302403407362E-4	0.0	0.0	29.18600547611804	0.0
26	2.4338302403407362E-4	0.0	0.0	29.57736537876483	0.0
27	2.4338302403407362E-4	0.0	0.0	30.000365074536052	0.0
28	2.4338302403407362E-4	0.0	0.0	30.391968360206874	0.0
29	2.4338302403407362E-4	0.0	0.0	31.01551566778217	0.0
30	2.4338302403407362E-4	0.0	0.0	31.637846060237298	0.0
31	2.4338302403407362E-4	0.0	0.0	32.078125950714934	0.0
32	2.4338302403407362E-4	0.0	0.0	32.502829327654396	0.0
33	2.4338302403407362E-4	0.0	0.0	32.919014298752664	0.0
34	2.4338302403407362E-4	0.0	0.0	33.61338606632187	0.0
35	2.4338302403407362E-4	0.0	0.0	34.06267112868878	0.0
36	2.4338302403407362E-4	0.0	0.0	34.51268634012778	0.0
37	2.4338302403407362E-4	0.0	0.0	34.94055369637968	0.0
38	2.4338302403407362E-4	0.0	0.0	35.31755400060846	0.0
39	2.4338302403407362E-4	0.0	0.0	35.83109218132035	0.0
40	2.4338302403407362E-4	0.0	0.0	36.29863097048981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGATA	35	1.0174335E-7	46.000004	19
GCCGGAT	35	1.0174335E-7	46.000004	18
CGGATAA	35	1.0174335E-7	46.000004	20
CCATAGG	35	1.0174335E-7	46.000004	1
CGAATGT	35	1.0174335E-7	46.000004	10
TTCGGTA	35	1.0174335E-7	46.000004	34
ACGTAGG	35	1.0174335E-7	46.000004	1
ATTGCGG	70	0.0	46.000004	1
AGGGCGC	35	1.0174335E-7	46.000004	5
TCGGTAC	35	1.0174335E-7	46.000004	35
GCCCGCG	35	1.0174335E-7	46.000004	5
GACTAAT	35	1.0174335E-7	46.000004	8
CTAGCGG	140	0.0	46.000004	1
AGGTACT	20	6.3066976E-4	46.0	22
GCCCAAT	20	6.3066976E-4	46.0	8
ACGCCGA	20	6.3066976E-4	46.0	41
CGTATAA	20	6.3066976E-4	46.0	21
CGAAATG	25	3.4132965E-5	46.0	45
ATAGGGT	20	6.3066976E-4	46.0	3
TTTACGG	25	3.4132965E-5	46.0	1
>>END_MODULE