FastQCFastQC Report
Thu 26 May 2016
SRR1544840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544840_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1179163
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT59480.5044255968004423No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT45810.388495907690455Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT39970.3389692519185219No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.1892868076762924No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19630.1664740158909328No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC14470.12271416250340283No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT14360.1217812974118082No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC13720.1163537186970758No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12730.10795793287272412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAA2100.046.00000411
TCGACAC351.0195981E-746.00000434
AACGTTC206.311522E-446.039
CTCACGT301.8614446E-646.044
TGGAACG206.311522E-446.026
TAAACGG650.046.01
GCGCAAA206.311522E-446.019
CATTACG206.311522E-446.014
ATATCGA206.311522E-446.042
TAACACG206.311522E-446.025
ATCCCGC206.311522E-446.09
ACTACGG600.046.01
GCGATCG206.311522E-446.08
GCGATAT600.046.08
ACCGTAG253.4172102E-546.039
ACGTACT206.311522E-446.026
CACAACG405.6115823E-946.011
AATCGAC253.4172102E-546.012
TACCGAT253.4172102E-546.026
CATGCGG1250.044.161
Produced by FastQC (version 0.11.5)