FastQCFastQC Report
Thu 26 May 2016
SRR1544839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544839_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences891099
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT74910.8406473354812429No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT56450.6334874127341631Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT42200.4735725211227933No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA36850.41353429865817376No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTG28070.31500428123025614Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT16990.19066343919138054No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC16230.1821346449721075No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC15350.17225919903400183No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC13640.15306941204063745No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13560.15217164422808238No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT11500.12905412305478964No Hit
AGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC11320.12703414547654077No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.1160364897727413No Hit
CTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTGAAA9300.10436550820952555Illumina Single End Adapter 2 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9000.10099887891244408No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG8960.10054999500616654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAA1100.046.00000411
ACTACGG351.01921614E-746.0000041
CGGGTAC453.092282E-1046.05
TTTACGG900.046.01
CGACGGT405.6097633E-946.027
CATGCGG2000.046.01
GACACTA253.4165336E-546.07
ATAGCGG600.046.01
ATTCGAT253.4165336E-546.013
TCGTCGG206.310688E-446.01
CGTAAGA206.310688E-446.017
CCGTTGG501.6370905E-1146.01
AGTCGAC206.310688E-446.016
GCGTCCC5600.043.946437
GTCCCCC5550.043.927939
CGAATAT1000.043.69999714
TCGGCGT5850.043.6410264
GTCGGCG5850.043.6410263
CGGGATA950.043.5789455
AATGCGG850.043.2941171
Produced by FastQC (version 0.11.5)