FastQCFastQC Report
Thu 26 May 2016
SRR1544838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544838_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences456590
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT28350.6209071595961365No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT26750.5858647802185768Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT18860.41306204691298537No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT9290.20346481526095622No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.19119998247881032No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC7840.1717076589500427No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC7590.166232287172299No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC7230.15834775181234806No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6090.13338005650583676No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT5610.12286734269256881No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG5310.11629689655927639No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT5300.11607788168816664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG750.046.0000044
GGTATGC750.046.0000047
CTTCGGG253.413898E-546.02
CGGGTCA206.3074374E-446.05
TCCGCGG206.3074374E-446.01
ACGCCAA206.3074374E-446.043
GTATTAC206.3074374E-446.08
ATTACGG206.3074374E-446.01
TAGCGCC206.3074374E-446.020
ACCGAGG405.5988494E-946.01
GGATCGT206.3074374E-446.08
CGATTGA253.413898E-546.09
AGACCGG301.8589217E-646.01
ACTACGG501.6370905E-1146.01
TCGTCCC301.8589217E-646.037
CGAATAT206.3074374E-446.014
ACTCGTA253.413898E-546.015
AATTCGC301.8589217E-646.012
AGTTCGG206.3074374E-446.01
CGTACAC253.413898E-546.018
Produced by FastQC (version 0.11.5)