##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544838_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	456590
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87275235988523	34.0	31.0	34.0	31.0	34.0
2	33.17362841936968	34.0	33.0	34.0	31.0	34.0
3	33.21277732758054	34.0	33.0	34.0	31.0	34.0
4	36.532994590332684	37.0	37.0	37.0	35.0	37.0
5	36.52162771852209	37.0	37.0	37.0	35.0	37.0
6	36.66124093825971	37.0	37.0	37.0	35.0	37.0
7	36.63212072099696	37.0	37.0	37.0	35.0	37.0
8	36.53927593683611	37.0	37.0	37.0	35.0	37.0
9	38.30886572198252	39.0	39.0	39.0	37.0	39.0
10	38.10255371339714	39.0	39.0	39.0	35.0	39.0
11	37.409157011761096	39.0	35.0	39.0	35.0	39.0
12	37.34442059615848	39.0	35.0	39.0	35.0	39.0
13	37.341792417705165	39.0	35.0	39.0	35.0	39.0
14	38.65513042335574	41.0	35.0	41.0	35.0	41.0
15	38.70018178234302	41.0	35.0	41.0	35.0	41.0
16	38.70121553253466	41.0	35.0	41.0	35.0	41.0
17	38.761856370047525	41.0	36.0	41.0	35.0	41.0
18	38.742066186294046	41.0	36.0	41.0	35.0	41.0
19	38.65027924396067	41.0	35.0	41.0	35.0	41.0
20	38.55702271184214	41.0	35.0	41.0	35.0	41.0
21	38.48947195514575	40.0	35.0	41.0	35.0	41.0
22	38.47306993144834	40.0	35.0	41.0	35.0	41.0
23	38.430642370616965	40.0	35.0	41.0	35.0	41.0
24	38.38331544711886	40.0	35.0	41.0	35.0	41.0
25	38.358336801068795	40.0	35.0	41.0	35.0	41.0
26	38.28756214546968	40.0	35.0	41.0	35.0	41.0
27	38.263897588646266	40.0	35.0	41.0	35.0	41.0
28	38.295475152762876	40.0	35.0	41.0	35.0	41.0
29	38.311717295604375	40.0	36.0	41.0	35.0	41.0
30	38.17700562868219	40.0	35.0	41.0	35.0	41.0
31	38.136790118049014	40.0	35.0	41.0	35.0	41.0
32	38.03149214831687	40.0	35.0	41.0	35.0	41.0
33	37.959217240850656	40.0	35.0	41.0	35.0	41.0
34	37.80496944742548	40.0	35.0	41.0	35.0	41.0
35	37.796281127488555	40.0	35.0	41.0	35.0	41.0
36	37.74877023149872	40.0	35.0	41.0	35.0	41.0
37	37.667741299634244	40.0	35.0	41.0	34.0	41.0
38	37.63542127510458	40.0	35.0	41.0	35.0	41.0
39	37.58249195120349	40.0	35.0	41.0	34.0	41.0
40	37.50955780897523	40.0	35.0	41.0	34.0	41.0
41	37.457760791957774	40.0	35.0	41.0	34.0	41.0
42	37.368166188484196	39.0	35.0	41.0	34.0	41.0
43	37.399686808734316	39.0	35.0	41.0	34.0	41.0
44	37.360673689743535	39.0	35.0	41.0	34.0	41.0
45	37.335524212094	39.0	35.0	41.0	34.0	41.0
46	37.29168619549267	39.0	35.0	41.0	34.0	41.0
47	37.21542083707484	39.0	35.0	41.0	34.0	41.0
48	37.222714032282795	39.0	35.0	41.0	34.0	41.0
49	37.16265139402965	39.0	35.0	41.0	34.0	41.0
50	37.07075275411201	39.0	35.0	41.0	34.0	41.0
51	37.00592435226352	39.0	35.0	41.0	34.0	41.0
52	36.76214546967739	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	3.0
17	14.0
18	30.0
19	55.0
20	121.0
21	187.0
22	337.0
23	572.0
24	998.0
25	1689.0
26	2334.0
27	2677.0
28	2722.0
29	3070.0
30	3490.0
31	4281.0
32	5366.0
33	7555.0
34	20947.0
35	91657.0
36	16505.0
37	22911.0
38	42324.0
39	224763.0
40	1979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.919665345276943	22.735714755031864	26.41319345583565	18.931426443855536
2	25.8706936200968	24.6461814757222	27.109660745964653	22.373464158216343
3	25.180577761229987	25.34549595917563	26.826255502748637	22.64767077684575
4	25.482161238748112	27.547690488184145	24.583105192842595	22.387043080225148
5	23.380494535578965	35.1875862371055	21.4783503799908	19.953568847324735
6	85.99706520072714	2.398431853522854	8.529972185111369	3.0745307606386474
7	86.9559123064456	2.222781926892836	5.815063842834928	5.006241923826628
8	76.7130248143849	4.375698109901662	11.979894434832126	6.931382640881316
9	48.81337742832738	20.831380450732603	14.905714097987252	15.449528022952757
10	39.95838717448915	20.44372412886835	26.69528460982501	12.902604086817496
11	36.57767362403907	18.612321776648635	27.096519853698066	17.713484745614227
12	19.815808493396702	34.233338443680324	29.248340962351342	16.702512100571628
13	13.5946910795243	36.858012659059554	30.088701022799448	19.458595238616702
14	12.630587616899188	24.598655248691387	43.96614030092643	18.804616833482992
15	14.058126546792526	18.974572373464156	46.34639392014718	20.620907159596136
16	15.20488841192317	22.69913927155654	27.353424297509797	34.742548019010485
17	18.47499945246282	23.37655226789899	33.50949429466261	24.638953984975583
18	23.79552771633194	26.967739109485535	28.055147944545432	21.181585229637093
19	25.88383451236339	23.0802251472875	26.302590945925232	24.733349394423882
20	18.5306292297247	31.028712849602485	28.020981624652315	22.419676296020498
21	19.986640092862306	24.389934076523794	27.426794279331567	28.19663155128233
22	16.833921023237476	30.15747169232791	24.72721697803281	28.281390306401804
23	17.03913795746731	23.73376552267899	38.096760770056285	21.13033574979741
24	18.489673448827176	22.911145666790773	34.80606233163232	23.793118552749732
25	15.868503471385706	32.54845704023303	28.516831292844785	23.06620819553648
26	18.196631551282334	28.647364156026196	28.542018003022406	24.613986289669068
27	19.73083072340612	25.112464136314856	36.44516962701768	18.711535513261353
28	14.964191068573557	23.527234499222498	34.062068814472504	27.446505617731443
29	21.003964169167087	26.363039050351517	29.13664337808537	23.49635340239602
30	26.009111018638166	23.6665279572483	27.302393832541227	23.02196719157231
31	27.25924790293261	24.55069099191835	24.646838520335532	23.54322258481351
32	23.17637267570468	25.786591909590662	30.021682472239863	21.015352942464794
33	20.178935149696663	23.70616964891916	30.25296217613176	25.861933025252416
34	17.14273199150222	22.0556735802361	35.709279660088924	25.09231476817276
35	26.451959088022075	23.226965110931033	28.93755886024661	21.38351694080028
36	21.71598151514488	24.35029238485293	33.37282901509012	20.560897084912067
37	19.40734575877702	27.804156902253663	31.314089226658492	21.474408112310826
38	17.735386232725205	27.56783985632624	30.447228366806105	24.24954554414245
39	25.153200902341272	23.485183643969425	29.509625703585275	21.851989750104032
40	17.25661972447929	20.703694780875622	34.118136621476594	27.92154887316849
41	21.47944545434635	24.654285025953264	26.350117172956043	27.516152346744345
42	21.25013688429444	22.029391795702928	30.83554173328369	25.884929586718936
43	23.493068179329377	24.837162443329902	29.047066295801482	22.622703081539235
44	18.2879607525351	28.381042072756742	28.247223986508686	25.083773188199476
45	22.065310234564926	25.40397292976193	28.501719266738213	24.028997568934933
46	23.057666615563196	21.908714601721456	30.40495849668192	24.628660286033423
47	20.17170765895004	20.19141899734992	35.46332595983267	24.173547383867362
48	19.362885739941742	18.318841849361572	32.52830767209093	29.78996473860575
49	20.41021485358856	23.08175825138527	33.157099367047024	23.350927527979152
50	19.392014717799338	24.685385137650847	30.50526730765019	25.417332836899625
51	19.930572285858208	20.210254276265356	31.868853895179484	27.99031954269695
52	21.09595041503318	19.45333888171007	36.12847412339298	23.322236579863773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	105.5
2	142.0
3	826.5
4	1511.0
5	1045.5
6	580.0
7	656.5
8	733.0
9	763.0
10	793.0
11	824.5
12	856.0
13	795.5
14	729.0
15	723.0
16	690.5
17	658.0
18	649.5
19	641.0
20	737.5
21	834.0
22	863.0
23	892.0
24	1117.0
25	1342.0
26	1537.0
27	1732.0
28	2200.5
29	2669.0
30	3076.0
31	3483.0
32	3948.0
33	4413.0
34	5446.0
35	6479.0
36	7360.0
37	8241.0
38	9287.5
39	12567.5
40	14801.0
41	21791.5
42	28782.0
43	39791.0
44	50800.0
45	60002.5
46	69205.0
47	68564.0
48	67923.0
49	58596.0
50	49269.0
51	41825.5
52	34382.0
53	29387.0
54	24392.0
55	21007.5
56	17623.0
57	15619.0
58	13615.0
59	12378.0
60	11141.0
61	10232.5
62	9324.0
63	7762.5
64	5170.0
65	4139.0
66	3544.5
67	2950.0
68	2172.5
69	1395.0
70	1158.5
71	922.0
72	824.5
73	727.0
74	671.5
75	616.0
76	473.0
77	330.0
78	221.5
79	113.0
80	81.5
81	50.0
82	36.0
83	22.0
84	14.0
85	6.0
86	3.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	456590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75746714410478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.827927398321	23.201435046355385
2	12.27904688182414	8.044609495845531
3	4.296418521618742	4.222193656777475
4	1.9245384763955178	2.5217202363236657
5	1.1572441749861089	1.8954194019907051
6	0.8279878688586843	1.6273671245913415
7	0.6554380986777005	1.5029344387700494
8	0.5747926708491505	1.5063001624010626
9	0.46474315004389444	1.3701427621209565
>10	6.717835692417147	43.44278104563601
>50	0.20852791364505793	4.506825983040072
>100	0.05747884799190699	3.159923216576019
>500	0.006015228278222824	1.3790865614364543
>1k	0.002005076092740942	1.619260868135262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	2835	0.6209071595961365	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	2675	0.5858647802185768	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	1886	0.41306204691298537	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	929	0.20346481526095622	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.19119998247881032	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	784	0.1717076589500427	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	759	0.166232287172299	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	723	0.15834775181234806	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	609	0.13338005650583676	No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	561	0.12286734269256881	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	531	0.11629689655927639	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	530	0.11607788168816664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6001007468407105	0.0
2	0.0	0.0	0.0	1.9932543419698197	0.0
3	0.0	0.0	0.0	3.151185965527059	0.0
4	0.0	0.0	0.0	3.836045467487242	0.0
5	0.0	0.0	0.0	7.268884557261438	0.0
6	0.0	0.0	0.0	7.819925972973565	0.0
7	0.0	0.0	0.0	10.083006636150595	0.0
8	0.0	0.0	0.0	12.71556538688977	0.0
9	0.0	0.0	0.0	14.753936792308197	0.0
10	0.0	0.0	0.0	16.08510917891325	0.0
11	0.0	0.0	0.0	17.403578702993933	0.0
12	0.0	0.0	0.0	18.528877110755822	0.0
13	0.0	0.0	0.0	18.93821590485994	0.0
14	0.0	0.0	0.0	19.28513546069778	0.0
15	0.0	0.0	0.0	20.426859983792898	0.0
16	0.0	0.0	0.0	21.75584222168685	0.0
17	0.0	0.0	0.0	23.57782693444885	0.0
18	0.0	0.0	0.0	24.35883396482621	0.0
19	0.0	0.0	0.0	25.25591887689174	0.0
20	0.0	0.0	0.0	26.3558115596049	0.0
21	0.0	0.0	0.0	27.31860093300335	0.0
22	0.0	0.0	0.0	28.421997853654265	0.0
23	0.0	0.0	0.0	29.175628025142906	0.0
24	0.0	0.0	0.0	29.823035984143324	0.0
25	0.0	0.0	0.0	30.49694474254802	0.0
26	0.0	0.0	0.0	31.086313760704353	0.0
27	0.0	0.0	0.0	31.710068113624914	0.0
28	0.0	0.0	0.0	32.32484285682998	0.0
29	0.0	0.0	0.0	33.1588514860159	0.0
30	0.0	0.0	0.0	33.89955978010907	0.0
31	0.0	0.0	0.0	34.505135898727524	0.0
32	0.0	0.0	0.0	35.07588865283953	0.0
33	0.0	0.0	0.0	35.65124071924484	0.0
34	0.0	0.0	0.0	36.505398716572856	0.0
35	0.0	0.0	0.0	37.05424998357388	0.0
36	0.0	0.0	0.0	37.63332530278806	0.0
37	0.0	0.0	0.0	38.166188484198074	0.0
38	0.0	0.0	0.0	38.69226220460369	0.0
39	0.0	0.0	0.0	39.3057228585821	0.0
40	0.0	0.0	0.0	39.89640596596509	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	75	0.0	46.000004	4
GGTATGC	75	0.0	46.000004	7
CTTCGGG	25	3.413898E-5	46.0	2
CGGGTCA	20	6.3074374E-4	46.0	5
TCCGCGG	20	6.3074374E-4	46.0	1
ACGCCAA	20	6.3074374E-4	46.0	43
GTATTAC	20	6.3074374E-4	46.0	8
ATTACGG	20	6.3074374E-4	46.0	1
TAGCGCC	20	6.3074374E-4	46.0	20
ACCGAGG	40	5.5988494E-9	46.0	1
GGATCGT	20	6.3074374E-4	46.0	8
CGATTGA	25	3.413898E-5	46.0	9
AGACCGG	30	1.8589217E-6	46.0	1
ACTACGG	50	1.6370905E-11	46.0	1
TCGTCCC	30	1.8589217E-6	46.0	37
CGAATAT	20	6.3074374E-4	46.0	14
ACTCGTA	25	3.413898E-5	46.0	15
AATTCGC	30	1.8589217E-6	46.0	12
AGTTCGG	20	6.3074374E-4	46.0	1
CGTACAC	25	3.413898E-5	46.0	18
>>END_MODULE