FastQCFastQC Report
Thu 26 May 2016
SRR1544837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544837_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences317883
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT31040.9764598924761626No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT26570.835842118011973Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT18130.5703356266299235No Hit
TGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTG13200.4152471192231104Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT8140.2560690568542514No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6790.21360060147916057No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6460.20321942349858282No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6120.19252366436707846No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC5640.17742376912260172No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT5430.17081756495314315No Hit
CTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTGAAA4430.13935944986048326Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG4260.1340115702947311No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.11765335044654794No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC3620.11387837663542875No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT3610.11356379548450216No Hit
GTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTGA3580.11262005203172237Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC3550.11167630857894256No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA3390.10664301016411698No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC3270.1028680363529978No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAT253.4115405E-546.021
ACCGGGC453.092282E-1046.03
TGCCATA206.304531E-446.015
TTTACGG253.4115405E-546.01
CGACGGT301.8571245E-646.027
CGACGGA253.4115405E-546.027
CTTCAGG206.304531E-446.01
ACCGAGG301.8571245E-646.01
TAAACGG206.304531E-446.01
TCGGGCG253.4115405E-546.04
GCACGGC206.304531E-446.09
GGATCCC405.5897544E-946.07
TCGGCGT253.4115405E-546.04
AACGGAC206.304531E-446.037
AGTGCGG600.046.01
TACGCGG301.8571245E-646.01
CACTAGG206.304531E-446.01
ACGGACC206.304531E-446.038
GGAACGC453.092282E-1046.07
TCGTCCC551.8189894E-1246.037
Produced by FastQC (version 0.11.5)