##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544837_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	317883
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.916173560712586	34.0	33.0	34.0	31.0	34.0
2	33.23598305036759	34.0	33.0	34.0	31.0	34.0
3	33.31928099332144	34.0	34.0	34.0	31.0	34.0
4	36.587904983909176	37.0	37.0	37.0	35.0	37.0
5	36.57026641877672	37.0	37.0	37.0	35.0	37.0
6	36.534234293749584	37.0	37.0	37.0	35.0	37.0
7	36.3831944457552	37.0	37.0	37.0	35.0	37.0
8	36.44952073561657	37.0	37.0	37.0	35.0	37.0
9	38.267290166507806	39.0	39.0	39.0	37.0	39.0
10	38.14661369120085	39.0	39.0	39.0	35.0	39.0
11	37.555883768556356	39.0	35.0	39.0	35.0	39.0
12	37.5440146217319	39.0	35.0	39.0	35.0	39.0
13	37.54623871046895	39.0	35.0	39.0	35.0	39.0
14	38.86859945325796	41.0	36.0	41.0	35.0	41.0
15	38.896452468361	41.0	36.0	41.0	35.0	41.0
16	38.913005728522755	41.0	36.0	41.0	35.0	41.0
17	38.983701550570494	41.0	36.0	41.0	35.0	41.0
18	38.96458445402868	41.0	36.0	41.0	35.0	41.0
19	38.89205776968256	41.0	36.0	41.0	35.0	41.0
20	38.81176722253156	41.0	35.0	41.0	35.0	41.0
21	38.752849318774516	41.0	35.0	41.0	35.0	41.0
22	38.729765353919525	41.0	35.0	41.0	35.0	41.0
23	38.70231185687816	41.0	35.0	41.0	35.0	41.0
24	38.647603048920516	41.0	35.0	41.0	35.0	41.0
25	38.59771991581808	41.0	35.0	41.0	35.0	41.0
26	38.535401389819526	41.0	35.0	41.0	35.0	41.0
27	38.51092697627744	40.0	36.0	41.0	35.0	41.0
28	38.574821553842135	40.0	36.0	41.0	35.0	41.0
29	38.62435235605553	40.0	36.0	41.0	35.0	41.0
30	38.52227706420287	40.0	35.0	41.0	35.0	41.0
31	38.498117231811705	40.0	35.0	41.0	35.0	41.0
32	38.45348445811824	40.0	35.0	41.0	35.0	41.0
33	38.39350012425955	40.0	35.0	41.0	35.0	41.0
34	38.2618227461047	40.0	35.0	41.0	35.0	41.0
35	38.260495213647786	40.0	35.0	41.0	35.0	41.0
36	38.23871676056914	40.0	35.0	41.0	35.0	41.0
37	38.18669447564041	40.0	35.0	41.0	35.0	41.0
38	38.15054280977592	40.0	35.0	41.0	35.0	41.0
39	38.1445594762853	40.0	35.0	41.0	35.0	41.0
40	38.1117297873746	40.0	35.0	41.0	35.0	41.0
41	38.09031624843103	40.0	35.0	41.0	35.0	41.0
42	38.036069874765246	40.0	35.0	41.0	35.0	41.0
43	37.96517272078091	40.0	35.0	41.0	35.0	41.0
44	37.83325626095136	40.0	35.0	41.0	35.0	41.0
45	37.81437195446123	40.0	35.0	41.0	35.0	41.0
46	37.76745532161204	40.0	35.0	41.0	35.0	41.0
47	37.71914509426424	40.0	35.0	41.0	35.0	41.0
48	37.691814283871736	40.0	35.0	41.0	35.0	41.0
49	37.65199460178745	40.0	35.0	41.0	35.0	41.0
50	37.56898607349245	39.0	35.0	41.0	35.0	41.0
51	37.507224356131026	39.0	35.0	41.0	35.0	41.0
52	37.10133288033648	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	4.0
15	2.0
16	10.0
17	13.0
18	20.0
19	46.0
20	66.0
21	110.0
22	185.0
23	294.0
24	539.0
25	898.0
26	1257.0
27	1408.0
28	1439.0
29	1453.0
30	1759.0
31	2268.0
32	3024.0
33	4123.0
34	11695.0
35	59792.0
36	10210.0
37	14963.0
38	28979.0
39	172122.0
40	1200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.99442247619407	22.62436179349006	25.337309639081045	21.043906091234827
2	25.394563408549686	24.901929326198633	26.976906597710475	22.726600667541202
3	24.887144012105082	25.32032225693101	26.821503509152738	22.97103022181117
4	25.533608277259244	26.910529974864968	24.870156629955044	22.685705117920744
5	23.198157812780174	34.24341660296398	21.93857488447007	20.61985069978577
6	83.27875350364758	2.4666308044154612	9.947055992299052	4.307559699637918
7	84.3388919822702	2.262782218615025	7.137846314524526	6.260479484590242
8	72.97055835008479	4.479635589194767	14.14451228911266	8.405293771607793
9	46.68573028441282	20.124385387076376	16.143990084402123	17.04589424410868
10	37.45717764083012	19.9365804399732	29.239059654023652	13.367182265173035
11	34.22580005851209	18.492023794918254	27.749203323235278	19.532972823334372
12	19.096334185848253	32.0271923946861	31.229100014785317	17.647373404680337
13	13.124954778959555	35.26108662621153	31.10767169052765	20.50628690430127
14	12.166426012086209	24.870471211105976	42.571008830292904	20.392093946514915
15	13.331320013967405	19.352403242702504	45.91878143845377	21.397495304876323
16	14.424174932286407	23.80435569061573	27.64633528688228	34.12513409021558
17	17.250057411060045	23.57785726194858	33.614568882261715	25.557516444729668
18	22.260705983018912	27.651368585297103	28.859989367157095	21.227936064526887
19	24.345749851360406	22.87948710689153	26.416952149061135	26.357810892686935
20	17.438491520465078	31.817681348168982	28.01754104497567	22.726286086390278
21	19.41940902784987	24.363680976963224	27.54755680549133	28.669353189695578
22	16.35507403667387	30.36274352513346	24.25955461600652	29.02262782218615
23	16.21917497947358	23.83864503606673	37.48391703865887	22.458262945800815
24	19.43859847805639	22.520864594835206	33.536238175680985	24.504298751427413
25	15.841992179512587	31.57828509231384	28.664005310129824	23.915717418043744
26	18.578219030272145	26.94733596952338	28.348480415750448	26.12596458445403
27	20.83062007090659	24.19129050625545	35.84620756693501	19.131881855902957
28	15.34054982493559	22.7656087302561	33.16440325528575	28.72943818952256
29	22.075417685123142	25.03248050383317	28.86848305823212	24.02361875281157
30	25.66982191561046	22.977636425980627	27.627460417826683	23.725081240582227
31	27.147409581512694	23.352617157885135	24.626670819137857	24.87330244146431
32	22.69451339014669	24.620379196119327	31.40463629700236	21.28047111673163
33	20.849494939962188	22.227045799869764	30.524123655558803	26.39933560460924
34	17.157570552687623	20.401531381042712	37.393317667192015	25.047580399077646
35	25.810754271225576	22.94900954124631	29.47216428686026	21.768071900667856
36	21.70861606314273	23.180541268328284	34.23429374958712	20.876548918941875
37	19.56726216878537	26.88033018437601	32.72493338744129	20.827474259397324
38	17.788620341446382	26.69944602259322	30.6908516655499	24.821081970410496
39	25.531091628051833	22.462981663064713	30.412132765828936	21.59379394305452
40	17.577536389174632	19.8834162254666	34.807460606575376	27.73158677878339
41	21.68722454487972	24.1576303231063	26.42167086632503	27.73347426568895
42	20.808913971492657	20.69377727025352	31.998565509951778	26.49874324830205
43	24.617862546911915	23.22521179175986	29.075792036692743	23.08113362463548
44	18.359899711529085	26.117785474529935	29.89087179874356	25.631443015197412
45	21.883208601906993	23.887090533309426	29.14248324068918	25.0872176240944
46	23.66468165960432	20.195795308336713	31.05670954407754	25.082813487981426
47	19.3961300226813	18.801886228580955	36.95353321819663	24.84845053054111
48	19.322832614515402	16.95718235954738	32.40028563968504	31.319699386252175
49	20.459099731662278	20.974069075729123	34.54918948166464	24.017641710943963
50	19.773312822642293	22.501360563477757	31.83875828528106	25.886568328598887
51	19.59777654042525	18.381605810943018	32.77274972238213	29.247867926249594
52	20.675846144650706	18.090932827486842	38.114337665115784	23.11888336274667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	64.5
2	88.0
3	412.5
4	737.0
5	533.0
6	329.0
7	353.5
8	378.0
9	425.5
10	473.0
11	479.0
12	485.0
13	476.5
14	446.5
15	425.0
16	420.5
17	416.0
18	387.0
19	358.0
20	422.5
21	487.0
22	523.0
23	559.0
24	681.0
25	803.0
26	924.5
27	1046.0
28	1309.5
29	1573.0
30	1784.5
31	1996.0
32	2373.0
33	2750.0
34	3301.0
35	3852.0
36	4316.0
37	4780.0
38	5649.5
39	7962.0
40	9405.0
41	14058.0
42	18711.0
43	26699.0
44	34687.0
45	43418.0
46	52149.0
47	51624.5
48	51100.0
49	43754.0
50	36408.0
51	30438.5
52	24469.0
53	20546.0
54	16623.0
55	14172.0
56	11721.0
57	10399.5
58	9078.0
59	8172.0
60	7266.0
61	6706.5
62	6147.0
63	5132.5
64	3423.5
65	2729.0
66	2314.0
67	1899.0
68	1408.5
69	918.0
70	772.5
71	627.0
72	581.0
73	535.0
74	454.5
75	374.0
76	308.0
77	242.0
78	156.0
79	70.0
80	50.5
81	31.0
82	20.5
83	10.0
84	6.0
85	2.0
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	317883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.490567850655836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.9734355166211	22.42978386360922
2	13.111291126939287	8.78209170192924
3	4.68227863126936	4.7043651058860725
4	2.2624977904656687	3.0308934305419752
5	1.6070113638001555	2.6909861558062036
6	1.235736314659766	2.4831306534979363
7	1.1024556465799906	2.5845305943888075
8	1.0308683587574499	2.7619493371248476
9	0.9503450094604607	2.8644834618780948
>10	6.8283639868141774	36.17024093297722
>50	0.1401658613050462	3.177430641724847
>100	0.0656095521002344	4.0746619471993295
>500	0.005964504736384946	1.2844224031616158
>1k	0.003976336490923297	2.961029770274601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	3104	0.9764598924761626	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	2657	0.835842118011973	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	1813	0.5703356266299235	No Hit
TGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTG	1320	0.4152471192231104	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	814	0.2560690568542514	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	679	0.21360060147916057	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	646	0.20321942349858282	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	612	0.19252366436707846	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	564	0.17742376912260172	No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	543	0.17081756495314315	No Hit
CTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTGAAA	443	0.13935944986048326	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	426	0.1340115702947311	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	374	0.11765335044654794	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	362	0.11387837663542875	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	361	0.11356379548450216	No Hit
GTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTTGA	358	0.11262005203172237	Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	355	0.11167630857894256	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	339	0.10664301016411698	No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	327	0.1028680363529978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8537732436147891	0.0
2	0.0	0.0	0.0	2.865519703790388	0.0
3	0.0	0.0	0.0	4.362296819899146	0.0
4	0.0	0.0	0.0	5.282761267510374	0.0
5	0.0	0.0	0.0	9.670539160634574	0.0
6	0.0	0.0	0.0	10.384323792087025	0.0
7	0.0	0.0	0.0	13.266516296876524	0.0
8	0.0	0.0	0.0	16.43025893174533	0.0
9	0.0	0.0	0.0	18.92992075700808	0.0
10	0.0	0.0	0.0	20.584617610881992	0.0
11	0.0	0.0	0.0	22.187408574853013	0.0
12	0.0	0.0	0.0	23.54356791649758	0.0
13	0.0	0.0	0.0	24.071120506601485	0.0
14	0.0	0.0	0.0	24.46938024367456	0.0
15	0.0	0.0	0.0	25.79848560633944	0.0
16	0.0	0.0	0.0	27.31256468574916	0.0
17	0.0	0.0	0.0	29.227420151439365	0.0
18	0.0	0.0	0.0	30.092518316487514	0.0
19	0.0	0.0	0.0	31.098548837150776	0.0
20	0.0	0.0	0.0	32.3134612420293	0.0
21	0.0	0.0	0.0	33.35441027044541	0.0
22	0.0	0.0	0.0	34.54132495289147	0.0
23	0.0	0.0	0.0	35.32305911294407	0.0
24	0.0	0.0	0.0	36.02897921562336	0.0
25	0.0	0.0	0.0	36.75817832347121	0.0
26	0.0	0.0	0.0	37.376015703891056	0.0
27	0.0	0.0	0.0	38.02719868630911	0.0
28	0.0	0.0	0.0	38.658563056218796	0.0
29	0.0	0.0	0.0	39.48811355121224	0.0
30	0.0	0.0	0.0	40.238389596172176	0.0
31	0.0	0.0	0.0	40.83861043214013	0.0
32	0.0	0.0	0.0	41.405800247260785	0.0
33	0.0	0.0	0.0	41.971417156626806	0.0
34	0.0	0.0	0.0	42.79153021709245	0.0
35	0.0	0.0	0.0	43.36312416832608	0.0
36	0.0	0.0	0.0	43.92937023999396	0.0
37	0.0	0.0	0.0	44.44150835370246	0.0
38	0.0	0.0	0.0	44.946411100939656	0.0
39	0.0	0.0	0.0	45.55323814107706	0.0
40	0.0	0.0	0.0	46.1012385059912	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGAT	25	3.4115405E-5	46.0	21
ACCGGGC	45	3.092282E-10	46.0	3
TGCCATA	20	6.304531E-4	46.0	15
TTTACGG	25	3.4115405E-5	46.0	1
CGACGGT	30	1.8571245E-6	46.0	27
CGACGGA	25	3.4115405E-5	46.0	27
CTTCAGG	20	6.304531E-4	46.0	1
ACCGAGG	30	1.8571245E-6	46.0	1
TAAACGG	20	6.304531E-4	46.0	1
TCGGGCG	25	3.4115405E-5	46.0	4
GCACGGC	20	6.304531E-4	46.0	9
GGATCCC	40	5.5897544E-9	46.0	7
TCGGCGT	25	3.4115405E-5	46.0	4
AACGGAC	20	6.304531E-4	46.0	37
AGTGCGG	60	0.0	46.0	1
TACGCGG	30	1.8571245E-6	46.0	1
CACTAGG	20	6.304531E-4	46.0	1
ACGGACC	20	6.304531E-4	46.0	38
GGAACGC	45	3.092282E-10	46.0	7
TCGTCCC	55	1.8189894E-12	46.0	37
>>END_MODULE