FastQCFastQC Report
Thu 26 May 2016
SRR1544836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544836_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences575184
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT22920.39848118167403823TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT20730.3604064090795293TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT18520.3219839216668058TruSeq Adapter, Index 27 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT8180.14221536064980947TruSeq Adapter, Index 27 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC7380.12830676792122173TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC6650.11561517705638542TruSeq Adapter, Index 27 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.10518373250994464No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC5880.10222815655511976TruSeq Adapter, Index 27 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG1150.046.0000041
GTTGATA301.8597693E-646.020
ATTTACC206.3088123E-446.09
AACGTAA206.3088123E-446.015
GGAATCG206.3088123E-446.07
GTCGCTA206.3088123E-446.021
AATCCCG253.4150118E-546.019
TTGGCCG206.3088123E-446.035
CCTTAAG206.3088123E-446.03
ACACGTG253.4150118E-546.037
TCGCAGG1050.046.01
TACCCCA206.3088123E-446.041
CTACTTT206.3088123E-446.043
CATATGG301.8597693E-646.01
TCACCGT206.3088123E-446.021
TACTTGA206.3088123E-446.033
ACTGATT206.3088123E-446.028
CACGGCG206.3088123E-446.03
GCGAATG253.4150118E-546.046
TAGACGC206.3088123E-446.011
Produced by FastQC (version 0.11.5)