##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544836_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	575184
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91908154607917	34.0	33.0	34.0	31.0	34.0
2	33.22293735569835	34.0	33.0	34.0	31.0	34.0
3	33.26878877020223	34.0	34.0	34.0	31.0	34.0
4	36.55576824111937	37.0	37.0	37.0	35.0	37.0
5	36.548968677849174	37.0	37.0	37.0	35.0	37.0
6	36.67446243289104	37.0	37.0	37.0	35.0	37.0
7	36.65800509054494	37.0	37.0	37.0	35.0	37.0
8	36.5815669420568	37.0	37.0	37.0	35.0	37.0
9	38.36587596317005	39.0	39.0	39.0	37.0	39.0
10	38.037473225959	39.0	39.0	39.0	35.0	39.0
11	36.73596275278867	38.0	35.0	39.0	35.0	39.0
12	36.51010111546913	37.0	35.0	39.0	35.0	39.0
13	36.47825217669476	37.0	35.0	39.0	35.0	39.0
14	37.53984464101922	38.0	35.0	41.0	35.0	41.0
15	37.663199949929066	38.0	35.0	41.0	35.0	41.0
16	37.68968886478066	38.0	35.0	41.0	35.0	41.0
17	37.83901325488887	38.0	35.0	41.0	35.0	41.0
18	37.80814834905004	38.0	35.0	41.0	35.0	41.0
19	37.66056601018109	38.0	35.0	41.0	35.0	41.0
20	37.54078868675067	38.0	35.0	41.0	35.0	41.0
21	37.46982530807533	38.0	35.0	41.0	35.0	41.0
22	37.46995222398398	38.0	35.0	41.0	35.0	41.0
23	37.43092645136165	37.0	35.0	41.0	35.0	41.0
24	37.391236543436534	37.0	35.0	41.0	35.0	41.0
25	37.34842415644385	37.0	35.0	41.0	35.0	41.0
26	37.29430582213691	37.0	35.0	41.0	35.0	41.0
27	37.32228121783638	36.0	35.0	41.0	35.0	41.0
28	37.38029917382959	36.0	35.0	41.0	35.0	41.0
29	37.395723803165595	36.0	35.0	41.0	35.0	41.0
30	37.28805564827951	36.0	35.0	41.0	35.0	41.0
31	37.207156318673675	36.0	35.0	41.0	35.0	41.0
32	37.111435644941444	36.0	35.0	41.0	35.0	41.0
33	37.08759979415283	36.0	35.0	41.0	35.0	41.0
34	36.989283429302624	36.0	35.0	41.0	35.0	41.0
35	36.90899086205458	36.0	35.0	41.0	35.0	41.0
36	36.83040035884169	36.0	35.0	41.0	34.0	41.0
37	36.77177737906479	36.0	35.0	41.0	34.0	41.0
38	36.77180519625024	36.0	35.0	41.0	34.0	41.0
39	36.619547831650394	36.0	35.0	41.0	34.0	41.0
40	36.62631262343876	36.0	35.0	41.0	34.0	41.0
41	36.61805091935798	36.0	35.0	41.0	34.0	41.0
42	36.492025160644246	35.0	35.0	40.0	34.0	41.0
43	36.48748922084064	35.0	35.0	41.0	34.0	41.0
44	36.44502454866617	35.0	35.0	40.0	34.0	41.0
45	36.46025967342624	35.0	35.0	41.0	34.0	41.0
46	36.43798853931959	35.0	35.0	41.0	34.0	41.0
47	36.37784083006481	35.0	35.0	40.0	34.0	41.0
48	36.43766168739047	36.0	35.0	40.0	34.0	41.0
49	36.321823972850424	35.0	35.0	40.0	34.0	41.0
50	36.23288373807338	35.0	35.0	40.0	34.0	41.0
51	36.171146972099365	36.0	35.0	40.0	33.0	41.0
52	35.953150643967845	35.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	12.0
17	25.0
18	47.0
19	114.0
20	187.0
21	316.0
22	491.0
23	780.0
24	1287.0
25	2051.0
26	2884.0
27	3245.0
28	3246.0
29	3687.0
30	4274.0
31	5486.0
32	7401.0
33	11352.0
34	40041.0
35	198007.0
36	18436.0
37	25545.0
38	44872.0
39	199618.0
40	1768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.13125538957968	22.568256418815544	25.649009708197724	20.651478483407047
2	25.11996161228407	23.36087234693594	28.61901582797853	22.900150212801467
3	25.40491390581101	25.52400623104954	26.466661103229576	22.604418759909873
4	25.35275668307881	26.97206459150463	25.124134190102644	22.551044535313917
5	23.027239978858937	35.92224401235083	21.240855100280953	19.809660908509276
6	87.9094341984478	2.6739269520709894	6.729324876908954	2.6873139725722552
7	89.63827227461125	1.8246335085816017	4.576274722523575	3.9608194942835686
8	81.7301941639545	3.2954671896297536	9.430025869982476	5.544312776433281
9	63.91676402681576	12.895004033491892	11.174684970374697	12.013546969317645
10	57.54419455339509	14.360621992266823	18.34908481459846	9.746098639739632
11	51.266377367937906	16.02391582519681	19.050425602937494	13.659281203927787
12	18.608132354168404	46.92985201257337	21.95732148321233	12.504694150045898
13	10.998045842721634	49.65854404851317	25.678043895518655	13.665366213246543
14	9.126644691090155	21.04057136498929	56.13890511558041	13.693878828340148
15	10.629815850232273	13.191604773429026	58.04629475089711	18.132284625441596
16	11.520139642270994	16.374412361957216	25.559820857325654	46.545627138446136
17	17.644440735486384	21.309702634287465	37.098041670143814	23.94781496008234
18	28.447070850371357	23.502044563131104	26.1145998497872	21.93628473671034
19	31.419337116470558	20.10295835767337	23.19362151937467	25.284083006481406
20	17.43546412973935	31.54660073993713	28.12647778797741	22.891457342346104
21	19.90371776683635	23.313061559431418	24.128974380372195	32.65424629336004
22	15.826761523269075	33.17095051322707	20.677383237364044	30.324904726139813
23	16.77567526217697	21.197390748004118	42.6117555425742	19.415178447244706
24	14.901144677181563	20.187974630726863	40.961501015327265	23.949379676764305
25	12.952029263679101	35.26575843556149	28.349884558680355	23.432327742079057
26	15.93072825391527	34.550509054493865	26.192835683885505	23.32592700770536
27	14.667480319341289	28.00495145901138	40.33665748699546	16.99091073465187
28	11.758672007566275	23.71519374669671	36.8515118640296	27.67462238170742
29	15.667160421708532	29.29792901054271	29.525856073882444	25.509054493866312
30	28.61588639461459	26.415547024952012	21.30326991015049	23.665296670282903
31	30.317428857548194	23.36087234693594	23.90174274666889	22.419956048846977
32	25.812957244985952	27.3050015299452	22.35945367047762	24.522587554591226
33	15.006502267100617	25.904753956994632	28.176548721800327	30.912195054104423
34	14.645922000611977	22.681437592144427	32.89017079751871	29.782469609724888
35	31.722370580544663	17.535432139976077	27.47868508164344	23.263512197835823
36	18.653335280536314	27.37002420095135	29.53385351470138	24.442787003810952
37	18.231209491223677	29.427278922918582	27.276141200033383	25.065370385824366
38	16.82296447745417	27.911068458093407	29.564452418704274	25.70151464574814
39	24.863521933850734	20.759443934462713	25.708990514339757	28.668043617346793
40	13.098764916965703	18.772427605774848	30.97061114356449	37.15819633369496
41	21.371422014520572	23.106692814821	23.19031822860163	32.3315669420568
42	21.198781607276977	23.010549667584634	26.529597485326434	29.261071239811955
43	20.221181395866367	27.288485076080004	28.393870483184514	24.09646304486912
44	14.896972099362987	35.709790258421656	22.685610169963002	26.70762747225236
45	20.83211633146958	29.592617318979663	27.22850426993797	22.346762079612784
46	21.92324543102729	22.336504492475452	27.87247211327158	27.86777796322568
47	22.26052880469554	18.203392305766503	34.06214359231133	25.473935297226625
48	19.01669726557067	16.171346908119837	32.252461820912956	32.55949400539653
49	18.812240952460428	28.10683190074828	29.31757489777184	23.763352249019444
50	17.099223900525747	30.431479317922612	25.009388300091796	27.459908481459845
51	19.040341873209268	21.49799020835072	32.24915853013992	27.212509388300095
52	25.61006564855768	18.838667278644746	31.66482377813013	23.886443294667444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	129.5
2	174.0
3	608.5
4	1043.0
5	796.5
6	550.0
7	669.0
8	788.0
9	846.0
10	904.0
11	891.5
12	879.0
13	842.0
14	802.0
15	799.0
16	729.5
17	660.0
18	688.5
19	717.0
20	789.5
21	862.0
22	797.5
23	733.0
24	815.0
25	897.0
26	1123.0
27	1349.0
28	1394.0
29	1439.0
30	2025.5
31	2612.0
32	2968.0
33	3324.0
34	4419.0
35	5514.0
36	6288.0
37	7062.0
38	7578.5
39	11218.5
40	14342.0
41	25582.0
42	36822.0
43	51764.5
44	66707.0
45	75538.5
46	84370.0
47	80025.0
48	75680.0
49	71693.0
50	67706.0
51	59256.0
52	50806.0
53	42954.0
54	35102.0
55	29997.0
56	24892.0
57	22726.0
58	20560.0
59	18272.0
60	15984.0
61	14635.0
62	13286.0
63	12112.0
64	9034.5
65	7131.0
66	5774.5
67	4418.0
68	3565.0
69	2712.0
70	2170.5
71	1629.0
72	1420.5
73	1212.0
74	924.0
75	636.0
76	535.5
77	435.0
78	377.0
79	319.0
80	200.0
81	81.0
82	77.0
83	73.0
84	62.0
85	51.0
86	26.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	575184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.161764361505046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34862348214075	14.675392289237985
2	12.513363068454545	5.2960968124918955
3	5.110824219201554	3.244621734594489
4	2.426888118751011	2.0542893800298065
5	1.429494839321803	1.5125316472857757
6	0.935920992260523	1.1883443699521907
7	0.6158982019523606	0.912344483427335
8	0.4423767981595803	0.7489178849320094
9	0.3099896896435838	0.5903935890060241
>10	4.198415223505374	24.61183731779844
>50	2.319570828722291	32.27840349767776
>100	0.3404120252006481	11.03195633625114
>500	0.005755758880204196	0.773094777715869
>1k	0.002466753805801798	1.0817758795992702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2292	0.39848118167403823	TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	2073	0.3604064090795293	TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	1852	0.3219839216668058	TruSeq Adapter, Index 27 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	818	0.14221536064980947	TruSeq Adapter, Index 27 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	738	0.12830676792122173	TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	665	0.11561517705638542	TruSeq Adapter, Index 27 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.10518373250994464	No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	588	0.10222815655511976	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36875156471668197	0.0
2	0.0	0.0	0.0	1.332617040807811	0.0
3	0.0	0.0	0.0	2.140010848702328	0.0
4	0.0	0.0	0.0	2.6287240257030793	0.0
5	0.0	0.0	0.0	5.186861943308576	0.0
6	0.0	0.0	0.0	5.623939469804445	0.0
7	0.0	0.0	0.0	7.4522239839773015	0.0
8	0.0	0.0	0.0	9.52077943753651	0.0
9	0.0	0.0	0.0	11.219192467106177	0.0
10	0.0	0.0	0.0	12.15732704664942	0.0
11	0.0	0.0	0.0	13.033742245959553	0.0
12	0.0	0.0	0.0	13.868779381902138	0.0
13	0.0	0.0	0.0	14.160685971793374	0.0
14	0.0	0.0	0.0	14.37383515535898	0.0
15	0.0	0.0	0.0	15.205916715346742	0.0
16	0.0	0.0	0.0	16.06233831260953	0.0
17	0.0	0.0	0.0	17.576288631116302	0.0
18	0.0	0.0	0.0	18.05231021725222	0.0
19	0.0	0.0	0.0	18.64846727308131	0.0
20	0.0	0.0	0.0	19.452557790202786	0.0
21	0.0	0.0	0.0	19.986821608389665	0.0
22	0.0	0.0	0.0	20.78830426437453	0.0
23	0.0	0.0	0.0	21.271454004283846	0.0
24	0.0	0.0	0.0	21.645073576455534	0.0
25	0.0	0.0	0.0	22.083541962224263	0.0
26	0.0	0.0	0.0	22.406221313527496	0.0
27	0.0	0.0	0.0	22.785230465381513	0.0
28	0.0	0.0	0.0	23.115211827867256	0.0
29	0.0	0.0	0.0	23.700415866922583	0.0
30	0.0	0.0	0.0	24.267191020612536	0.0
31	0.0	0.0	0.0	24.651068179921555	0.0
32	0.0	0.0	0.0	25.0379009151854	0.0
33	0.0	0.0	0.0	25.410477342902446	0.0
34	0.0	0.0	0.0	26.05496675846338	0.0
35	0.0	0.0	0.0	26.44684135859134	0.0
36	0.0	0.0	0.0	26.85210297922056	0.0
37	0.0	0.0	0.0	27.18834320843417	0.0
38	0.0	0.0	0.0	27.513804278283125	0.0
39	0.0	0.0	0.0	27.914719463684666	0.0
40	0.0	0.0	0.0	28.30224762858494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGG	115	0.0	46.000004	1
GTTGATA	30	1.8597693E-6	46.0	20
ATTTACC	20	6.3088123E-4	46.0	9
AACGTAA	20	6.3088123E-4	46.0	15
GGAATCG	20	6.3088123E-4	46.0	7
GTCGCTA	20	6.3088123E-4	46.0	21
AATCCCG	25	3.4150118E-5	46.0	19
TTGGCCG	20	6.3088123E-4	46.0	35
CCTTAAG	20	6.3088123E-4	46.0	3
ACACGTG	25	3.4150118E-5	46.0	37
TCGCAGG	105	0.0	46.0	1
TACCCCA	20	6.3088123E-4	46.0	41
CTACTTT	20	6.3088123E-4	46.0	43
CATATGG	30	1.8597693E-6	46.0	1
TCACCGT	20	6.3088123E-4	46.0	21
TACTTGA	20	6.3088123E-4	46.0	33
ACTGATT	20	6.3088123E-4	46.0	28
CACGGCG	20	6.3088123E-4	46.0	3
GCGAATG	25	3.4150118E-5	46.0	46
TAGACGC	20	6.3088123E-4	46.0	11
>>END_MODULE