FastQCFastQC Report
Thu 26 May 2016
SRR1544835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544835_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences326508
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT19350.5926347899592047TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT15790.48360223945508224TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT11130.3408798559300231TruSeq Adapter, Index 27 (96% over 26bp)
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTTG7980.24440442500643172TruSeq Adapter, Index 27 (96% over 28bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6400.19601357393999536No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT4810.14731645166427776TruSeq Adapter, Index 27 (96% over 26bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4660.14272238352505912No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC4510.13812831538584047TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC4450.13629068813015302TruSeq Adapter, Index 27 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC3850.11791441557327846TruSeq Adapter, Index 27 (100% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC3300.1010694990628101TruSeq Adapter, Index 27 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGG750.046.0000041
CCATAGG750.046.0000041
GTTGATA206.3047843E-446.020
GTGTTAC206.3047843E-446.036
ACCGGTA206.3047843E-446.041
ACACGAG206.3047843E-446.037
ATCTATG206.3047843E-446.026
GTACAGG950.046.01
AAATAGG600.046.01
TTTACGG253.4117467E-546.01
TCATTGA206.3047843E-446.015
GACACGA206.3047843E-446.036
GGCACGC206.3047843E-446.033
CTATAGG600.046.01
CGTGAAG206.3047843E-446.09
CACGCTA206.3047843E-446.035
ATAGCGG453.092282E-1046.01
GTCTCAT206.3047843E-446.012
CCGTGGG301.8572828E-646.02
GCACGCT206.3047843E-446.034
Produced by FastQC (version 0.11.5)