##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544835_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	326508
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97726548813505	34.0	33.0	34.0	31.0	34.0
2	33.288045009616916	34.0	34.0	34.0	31.0	34.0
3	33.36612272899898	34.0	34.0	34.0	31.0	34.0
4	36.60348597890404	37.0	37.0	37.0	35.0	37.0
5	36.595351415585526	37.0	37.0	37.0	35.0	37.0
6	36.55355764636701	37.0	37.0	37.0	35.0	37.0
7	36.46296874808581	37.0	37.0	37.0	35.0	37.0
8	36.50970879733421	37.0	37.0	37.0	35.0	37.0
9	38.34522890710182	39.0	39.0	39.0	37.0	39.0
10	38.0754652259669	39.0	39.0	39.0	35.0	39.0
11	36.85550124346111	38.0	35.0	39.0	35.0	39.0
12	36.68917760054884	39.0	35.0	39.0	35.0	39.0
13	36.65649539980644	39.0	35.0	39.0	35.0	39.0
14	37.707658005316866	39.0	35.0	41.0	35.0	41.0
15	37.816316904945666	39.0	35.0	41.0	35.0	41.0
16	37.84857645141926	39.0	35.0	41.0	35.0	41.0
17	38.00278094258027	39.0	35.0	41.0	35.0	41.0
18	37.98068041211854	39.0	35.0	41.0	35.0	41.0
19	37.833465029953324	39.0	35.0	41.0	35.0	41.0
20	37.72284293187303	39.0	35.0	41.0	35.0	41.0
21	37.65786136939983	39.0	35.0	41.0	35.0	41.0
22	37.66630526663972	39.0	35.0	41.0	35.0	41.0
23	37.64135335121957	38.0	35.0	41.0	35.0	41.0
24	37.58495657074253	38.0	35.0	41.0	35.0	41.0
25	37.53017690225048	38.0	35.0	41.0	35.0	41.0
26	37.482548666495155	38.0	35.0	41.0	35.0	41.0
27	37.51562289438544	38.0	35.0	41.0	35.0	41.0
28	37.59079716270352	38.0	35.0	41.0	35.0	41.0
29	37.64478971418771	38.0	35.0	41.0	35.0	41.0
30	37.530838448062525	38.0	35.0	41.0	35.0	41.0
31	37.474052703149695	38.0	35.0	41.0	35.0	41.0
32	37.421640511105394	38.0	35.0	41.0	35.0	41.0
33	37.39378820733336	38.0	35.0	41.0	35.0	41.0
34	37.31228943854362	38.0	35.0	41.0	35.0	41.0
35	37.238894605951465	37.0	35.0	41.0	35.0	41.0
36	37.17197434672351	37.0	35.0	41.0	35.0	41.0
37	37.12641956705502	37.0	35.0	41.0	35.0	41.0
38	37.11917625295552	37.0	35.0	41.0	35.0	41.0
39	37.05044286816862	36.0	35.0	41.0	35.0	41.0
40	37.06011185024563	36.0	35.0	41.0	35.0	41.0
41	37.03685055190072	36.0	35.0	41.0	35.0	41.0
42	36.94033224300783	36.0	35.0	41.0	35.0	41.0
43	36.87656657723547	36.0	35.0	41.0	35.0	41.0
44	36.74266174182562	35.0	35.0	41.0	34.0	41.0
45	36.753849829100666	35.0	35.0	41.0	34.0	41.0
46	36.722597914905606	35.0	35.0	41.0	35.0	41.0
47	36.680473372781066	35.0	35.0	41.0	35.0	41.0
48	36.69908241145699	36.0	35.0	40.0	35.0	41.0
49	36.60936332341014	35.0	35.0	40.0	34.0	41.0
50	36.527653227486006	35.0	35.0	40.0	34.0	41.0
51	36.476558001641614	36.0	35.0	40.0	34.0	41.0
52	36.120664731032626	35.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	4.0
16	8.0
17	18.0
18	30.0
19	50.0
20	99.0
21	153.0
22	224.0
23	361.0
24	619.0
25	985.0
26	1291.0
27	1568.0
28	1493.0
29	1580.0
30	1900.0
31	2445.0
32	3329.0
33	5288.0
34	19591.0
35	112243.0
36	9755.0
37	13871.0
38	25657.0
39	122449.0
40	1490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.84245408994573	22.214157080377817	24.631862006443946	23.31152682323251
2	24.544880983008074	24.006149925882365	28.65044654342313	22.798522547686428
3	25.292795276072866	25.500753427174832	26.59628554277384	22.61016575397846
4	25.327403922721647	26.657233513420802	25.36507528146324	22.650287282394306
5	23.066816127016796	35.39913263993532	21.47083685545224	20.06321437759565
6	86.7154862974261	2.8519975008269323	7.212993249782547	3.2195229519644237
7	88.49002168400162	1.8808114961961115	5.175677165643721	4.453489654158551
8	79.97170054026242	3.667597731142882	10.219657711296508	6.141044017298198
9	62.777328580004166	12.983142832641159	11.722836806448846	12.516691780905829
10	55.97841400516986	14.42506768593725	19.465985519497224	10.130532789395666
11	49.822975241035444	16.236968160045084	19.54010315214329	14.39995344677619
12	18.37811018413025	45.704546289830574	22.440797775245937	13.476545750793242
13	11.059759638354956	48.44996140982763	26.1136021169466	14.376676834870814
14	9.281548997268061	21.163034290124592	54.761904761904766	14.793511950702587
15	10.56758180504	13.605792201109926	57.12509341271883	18.701532581131243
16	11.594815440969288	17.212748232815123	25.84377718156982	45.348659144645765
17	17.482879439401177	21.477881093265708	36.804304948117654	24.234934519215457
18	27.77726732576231	23.82636872603428	26.081137368762786	22.315226579440626
19	30.73247822411702	20.238095238095237	23.115206978083233	25.91421955970451
20	17.3208619696914	31.88865203915371	27.800850208876966	22.989635782277922
21	19.824935376774842	23.593908878189815	24.132946206524803	32.44820953851054
22	15.843409656118688	33.591519962757424	20.63073492839379	29.934335452730103
23	16.648596665319072	21.651230597718893	41.79499430335551	19.905178433606526
24	15.101620787239517	20.669325100763228	39.83608364879268	24.392970463204577
25	13.309627941734965	35.052433631028954	28.05138005806902	23.586558369167065
26	16.2219608707903	33.33792740147255	25.847146164871916	24.592965562865228
27	15.263331985740011	27.45231357271491	39.517255319930904	17.767099121614173
28	12.071679713820183	23.73724380413344	36.56388204883188	27.627194433214502
29	16.047386281499996	28.099770909135458	29.25655726659071	26.59628554277384
30	27.999926494909772	26.278069756330623	21.285236502627807	24.436767246131794
31	29.7514915407892	23.21842037561101	23.554399892192535	23.475688191407254
32	25.13690323054871	26.792911659132397	22.885809842331582	25.184375267987306
33	14.777279576610681	25.498609528709864	28.57479755473066	31.14931333994879
34	14.984012642875518	22.298075391720875	33.121699927719995	29.59621203768361
35	30.598331434451836	17.47062859102993	27.896406826172715	24.03463314834552
36	18.689588003969273	26.948803704656548	29.048292844279466	25.31331544709471
37	18.471216631751748	28.967130973819938	27.89273157166134	24.668920822766978
38	16.828071593957883	27.596261041077096	29.133436240459652	26.442231124505373
39	24.823587783454006	20.31006897227633	25.94913447756257	28.91720876670709
40	13.42907371335465	18.404143236919158	30.67122398226077	37.49555906746542
41	21.435003123966332	23.389625981599227	22.931137981305206	32.24423291312923
42	21.21142514119103	22.94890171144352	26.235804329449813	29.60386881791564
43	20.772538498291006	27.010976760140636	28.142036336016268	24.074448405552083
44	15.2798706310412	34.17557915885675	23.502946329033286	27.041603881068767
45	20.62950984355667	28.919965207590625	27.5879917184265	22.86253323042621
46	22.201293689588002	21.487069229544144	28.10773396057677	28.20390312029108
47	22.066228086294977	17.669092334644173	34.24142746885222	26.023252110208634
48	18.697857326619868	16.0195156014554	32.26720325382533	33.01542381809941
49	18.43477035784728	27.390446788440098	29.648278143261418	24.5265047104512
50	17.241231455278278	29.78365001776373	25.558332414519707	27.416786112438285
51	19.145625834589044	20.89627206684063	31.926629669104585	28.031472429465744
52	25.46614478052605	18.95359378636971	31.76522474181337	23.81503669129087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	65.0
2	78.0
3	278.5
4	479.0
5	367.0
6	255.0
7	334.0
8	413.0
9	447.0
10	481.0
11	499.0
12	517.0
13	482.5
14	434.5
15	421.0
16	412.0
17	403.0
18	407.5
19	412.0
20	486.0
21	560.0
22	499.0
23	438.0
24	467.0
25	496.0
26	603.0
27	710.0
28	732.0
29	754.0
30	1096.5
31	1439.0
32	1615.5
33	1792.0
34	2331.5
35	2871.0
36	3371.0
37	3871.0
38	4343.0
39	6313.5
40	7812.0
41	13659.0
42	19506.0
43	27920.5
44	36335.0
45	41314.0
46	46293.0
47	44997.0
48	43701.0
49	41793.0
50	39885.0
51	34883.5
52	29882.0
53	25331.0
54	20780.0
55	17714.5
56	14649.0
57	13099.5
58	11550.0
59	10315.0
60	9080.0
61	8417.0
62	7754.0
63	7210.0
64	5455.5
65	4245.0
66	3403.0
67	2561.0
68	2066.0
69	1571.0
70	1255.0
71	939.0
72	825.5
73	712.0
74	532.0
75	352.0
76	308.0
77	264.0
78	210.0
79	156.0
80	100.0
81	44.0
82	41.0
83	38.0
84	33.0
85	28.0
86	14.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	326508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.55526970242689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71740172579099	18.32757543459885
2	11.275167785234899	5.762799073835863
3	3.917785234899329	3.0036017494211475
4	1.7953020134228188	1.8351770860132062
5	0.9887344199424737	1.2633687382851262
6	0.7058964525407478	1.082362453599912
7	0.462607861936721	0.8275448074779179
8	0.39669223394055614	0.8110061621767308
9	0.30560882070949186	0.702892425300452
>10	7.800814956855226	51.20333958126601
>50	0.5225311601150527	8.97834050007963
>100	0.10546500479386386	4.344457103654428
>500	0.002396931927133269	0.440417998946427
>1k	0.0035953978906999038	1.4171168853443101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	1935	0.5926347899592047	TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	1579	0.48360223945508224	TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	1113	0.3408798559300231	TruSeq Adapter, Index 27 (96% over 26bp)
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTTG	798	0.24440442500643172	TruSeq Adapter, Index 27 (96% over 28bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	640	0.19601357393999536	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	481	0.14731645166427776	TruSeq Adapter, Index 27 (96% over 26bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	466	0.14272238352505912	No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	451	0.13812831538584047	TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	445	0.13629068813015302	TruSeq Adapter, Index 27 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	385	0.11791441557327846	TruSeq Adapter, Index 27 (100% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	330	0.1010694990628101	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4924841045242383	0.0
2	0.0	0.0	0.0	1.6802038541168975	0.0
3	0.0	0.0	0.0	2.623212907493844	0.0
4	0.0	0.0	0.0	3.1588812525267373	0.0
5	0.0	0.0	0.0	5.922978916289953	0.0
6	0.0	0.0	0.0	6.393717764955223	0.0
7	0.0	0.0	0.0	8.393668761561738	0.0
8	0.0	0.0	0.0	10.608009604665122	0.0
9	0.0	0.0	0.0	12.425116689330736	0.0
10	0.0	0.0	0.0	13.454800494934274	0.0
11	0.0	0.0	0.0	14.423230058681563	0.0
12	0.0	0.0	0.0	15.34388131378098	0.0
13	0.0	0.0	0.0	15.661484557805627	0.0
14	0.0	0.0	0.0	15.884449998162372	0.0
15	0.0	0.0	0.0	16.75854802945104	0.0
16	0.0	0.0	0.0	17.634789959204674	0.0
17	0.0	0.0	0.0	19.066607862594484	0.0
18	0.0	0.0	0.0	19.554804170188785	0.0
19	0.0	0.0	0.0	20.133963026939615	0.0
20	0.0	0.0	0.0	20.953851054185503	0.0
21	0.0	0.0	0.0	21.49840126428755	0.0
22	0.0	0.0	0.0	22.281230475210407	0.0
23	0.0	0.0	0.0	22.76942678280471	0.0
24	0.0	0.0	0.0	23.143383929337105	0.0
25	0.0	0.0	0.0	23.586252097957782	0.0
26	0.0	0.0	0.0	23.914268563097995	0.0
27	0.0	0.0	0.0	24.290063336886078	0.0
28	0.0	0.0	0.0	24.614098276305633	0.0
29	0.0	0.0	0.0	25.166305266639714	0.0
30	0.0	0.0	0.0	25.701361069254045	0.0
31	0.0	0.0	0.0	26.078993470297817	0.0
32	0.0	0.0	0.0	26.455400786504466	0.0
33	0.0	0.0	0.0	26.778516912296176	0.0
34	0.0	0.0	0.0	27.393815771742194	0.0
35	0.0	0.0	0.0	27.763178850135372	0.0
36	0.0	0.0	0.0	28.183689220478517	0.0
37	0.0	0.0	0.0	28.508336702316637	0.0
38	0.0	0.0	0.0	28.844316218898157	0.0
39	0.0	0.0	0.0	29.22194861994193	0.0
40	0.0	0.0	0.0	29.598662207357858	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAGG	75	0.0	46.000004	1
CCATAGG	75	0.0	46.000004	1
GTTGATA	20	6.3047843E-4	46.0	20
GTGTTAC	20	6.3047843E-4	46.0	36
ACCGGTA	20	6.3047843E-4	46.0	41
ACACGAG	20	6.3047843E-4	46.0	37
ATCTATG	20	6.3047843E-4	46.0	26
GTACAGG	95	0.0	46.0	1
AAATAGG	60	0.0	46.0	1
TTTACGG	25	3.4117467E-5	46.0	1
TCATTGA	20	6.3047843E-4	46.0	15
GACACGA	20	6.3047843E-4	46.0	36
GGCACGC	20	6.3047843E-4	46.0	33
CTATAGG	60	0.0	46.0	1
CGTGAAG	20	6.3047843E-4	46.0	9
CACGCTA	20	6.3047843E-4	46.0	35
ATAGCGG	45	3.092282E-10	46.0	1
GTCTCAT	20	6.3047843E-4	46.0	12
CCGTGGG	30	1.8572828E-6	46.0	2
GCACGCT	20	6.3047843E-4	46.0	34
>>END_MODULE