FastQCFastQC Report
Thu 26 May 2016
SRR1544834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544834_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences715521
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT21290.2975454249421051Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT16100.22501086620797991Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT14570.20362784600312223Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.14283298463636987No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC7230.10104525234060216Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG351.01885234E-746.00000411
AATCCTC206.3098484E-446.00000435
CGCGGAT206.3098484E-446.00000436
TTAGCCG206.3098484E-446.00000426
TTCACGG750.046.0000041
ATTCGTC206.3098484E-446.00000433
ATTCGAT206.3098484E-446.00000413
AATGGGT351.01885234E-746.0000043
CGCGACG206.3098484E-446.00000425
CTAACGG700.046.0000041
ATATCCG206.3098484E-446.00000415
CAGATAG351.01885234E-746.00000422
TTGACGT206.3098484E-446.00000446
AACGCTC206.3098484E-446.00000421
ACGCTCG206.3098484E-446.00000422
CGTCGCA206.3098484E-446.00000437
CACGTCA206.3098484E-446.00000434
CACAACG405.6061253E-946.00000411
ATTGACG206.3098484E-446.00000445
CGTTTAG405.6061253E-946.00000429