FastQCFastQC Report
Thu 26 May 2016
SRR1544833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544833_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences521682
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT27520.5275244305918165Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT20260.38835919199819047Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT17910.3433125927289038Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA16070.3080420639393347No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG10340.19820503678486126Illumina Single End Adapter 1 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC8220.15756725361427076Illumina Single End Adapter 1 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6730.12900579280097837No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT6230.1194214099777259Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC6080.11654609513075014Illumina Single End Adapter 1 (95% over 21bp)
ACTGTGGACTTAGGCATTTAGATGCTTGAGCCTATGAGGGCCATTTTTATTC5600.10734508762042777No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC5580.10696171230749768Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG206.3082675E-446.00000445
CGGACAA206.3082675E-446.00000434
AAGTAGT206.3082675E-446.00000413
TTAGGCA551.8189894E-1246.00000410
CGAACTC206.3082675E-446.00000412
TCCGCTT206.3082675E-446.00000412
GTCGAAC206.3082675E-446.00000410
TAGACCC206.3082675E-446.00000413
GGGTCGT206.3082675E-446.00000428
AGCTTAC206.3082675E-446.00000410
GACCGGC206.3082675E-446.00000433
GGCAACC206.3082675E-446.0000047
ATTCGTC206.3082675E-446.00000433
TTCGTCT206.3082675E-446.00000434
AGGGTAC206.3082675E-446.0000045
GTAGCGA206.3082675E-446.00000428
CTAACGG700.046.0000041
AGTGCCC206.3082675E-446.0000048
TGACCGT206.3082675E-446.00000418
CCATCGA206.3082675E-446.00000411
Produced by FastQC (version 0.11.5)