##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544833_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	521682
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.938061884443016	34.0	33.0	34.0	31.0	34.0
2	33.293981774337624	34.0	34.0	34.0	31.0	34.0
3	33.38178430538144	34.0	34.0	34.0	31.0	34.0
4	36.6043585939327	37.0	37.0	37.0	35.0	37.0
5	36.58983250332578	37.0	37.0	37.0	35.0	37.0
6	36.5493729896757	37.0	37.0	37.0	35.0	37.0
7	36.46830252912694	37.0	37.0	37.0	35.0	37.0
8	36.497310622179796	37.0	37.0	37.0	35.0	37.0
9	38.3251195172538	39.0	39.0	39.0	37.0	39.0
10	38.12121368956567	39.0	39.0	39.0	35.0	39.0
11	37.163653336707036	39.0	35.0	39.0	35.0	39.0
12	37.076565800621836	39.0	35.0	39.0	35.0	39.0
13	37.070613898888595	39.0	35.0	39.0	35.0	39.0
14	38.20247008714121	40.0	35.0	41.0	35.0	41.0
15	38.275422575438675	40.0	35.0	41.0	35.0	41.0
16	38.28500504138537	40.0	35.0	41.0	35.0	41.0
17	38.38918728267412	40.0	36.0	41.0	35.0	41.0
18	38.39998696523936	40.0	36.0	41.0	35.0	41.0
19	38.28320126053803	40.0	35.0	41.0	35.0	41.0
20	38.20458823574515	40.0	35.0	41.0	35.0	41.0
21	38.151743782610865	40.0	35.0	41.0	35.0	41.0
22	38.137723747417006	40.0	35.0	41.0	35.0	41.0
23	38.10729716570631	40.0	35.0	41.0	35.0	41.0
24	38.0447801534268	40.0	35.0	41.0	35.0	41.0
25	37.99705759447326	40.0	35.0	41.0	35.0	41.0
26	37.937946871849135	40.0	35.0	41.0	35.0	41.0
27	37.95736483144904	40.0	35.0	41.0	35.0	41.0
28	38.0082042316967	40.0	35.0	41.0	35.0	41.0
29	38.06041611556466	40.0	35.0	41.0	35.0	41.0
30	37.975835854026016	40.0	35.0	41.0	35.0	41.0
31	37.92919824720807	40.0	35.0	41.0	35.0	41.0
32	37.87855245149344	40.0	35.0	41.0	35.0	41.0
33	37.8288938472096	40.0	35.0	41.0	35.0	41.0
34	37.75153062593687	40.0	35.0	41.0	35.0	41.0
35	37.65711487074501	39.0	35.0	41.0	35.0	41.0
36	37.6214724679019	39.0	35.0	41.0	35.0	41.0
37	37.56389716340606	39.0	35.0	41.0	35.0	41.0
38	37.527564684999675	39.0	35.0	41.0	35.0	41.0
39	37.47336116638105	39.0	35.0	41.0	35.0	41.0
40	37.437810773613045	39.0	35.0	41.0	35.0	41.0
41	37.41230481404381	39.0	35.0	41.0	35.0	41.0
42	37.33999064564237	38.0	35.0	41.0	35.0	41.0
43	37.26852565355907	38.0	35.0	41.0	35.0	41.0
44	37.135764699567936	38.0	35.0	41.0	35.0	41.0
45	37.12029358881464	38.0	35.0	41.0	35.0	41.0
46	37.0771101935662	37.0	35.0	41.0	35.0	41.0
47	37.0265219041485	37.0	35.0	41.0	35.0	41.0
48	37.00762725185074	37.0	35.0	41.0	35.0	41.0
49	36.940289295011134	37.0	35.0	41.0	35.0	41.0
50	36.86738472862778	37.0	35.0	41.0	34.0	41.0
51	36.80647405890945	36.0	35.0	41.0	34.0	41.0
52	36.386386726013164	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	3.0
15	8.0
16	10.0
17	21.0
18	39.0
19	77.0
20	126.0
21	189.0
22	299.0
23	502.0
24	827.0
25	1350.0
26	1923.0
27	2248.0
28	2202.0
29	2420.0
30	2957.0
31	3574.0
32	5002.0
33	7628.0
34	27715.0
35	144160.0
36	17287.0
37	27756.0
38	47584.0
39	223513.0
40	2255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.30125248714734	22.305734144555494	24.4507190203994	21.94229434789776
2	25.042267128250543	24.583558566329682	27.078565102878766	23.295609202541012
3	26.32829961547456	25.14807871461925	25.767038157344896	22.756583512561292
4	26.541264601807228	26.883235380940878	23.657898873259956	22.917601143991934
5	23.95750668031483	34.20263685540233	21.446973443592075	20.392883020690768
6	87.39730333804884	2.7188977193002635	6.90094732039825	2.9828516222526367
7	88.82806000590398	2.0543166143359364	5.109817858388827	4.007805521371257
8	80.21246659842586	4.304921388892083	9.865588615286708	5.617023397395348
9	57.75529920526298	16.492422586939938	12.21587097120468	13.536407236592407
10	48.56368439010738	17.05138379319202	20.537415513665415	13.847516303035182
11	42.873052932629456	17.5001245969767	22.819457063881828	16.807365406512012
12	19.81034423269348	38.462128269712196	25.099198362220665	16.628329135373658
13	13.867643506964011	40.50839400247661	28.5520681181256	17.071894372433782
14	12.359061650584072	21.974114498870957	47.815144091611366	17.851679758933603
15	14.906398917348115	16.174604452520885	49.05536322894024	19.863633401190764
16	15.435840224504583	19.414317534436687	25.58991876277119	39.55992347828754
17	20.265794104454436	22.302283766739126	33.10925046292569	24.322671665880748
18	26.776273668633383	25.53950490912088	25.17683186308901	22.507389559156728
19	30.29623410430109	21.84587545669584	23.577773432857565	24.280117006145506
20	20.661437427398297	29.3665489704456	25.97942808070817	23.992585521447932
21	21.501221050371683	23.999486277080674	25.80173362316507	28.697559049382576
22	18.670952802665226	29.220674663875695	22.837859078902472	29.270513454556607
23	19.849256826955887	21.366464627876752	38.36570937851028	20.418569166657083
24	18.857656580062184	21.101935661954986	34.97398798501769	25.066419772965137
25	16.58979991642418	33.320681948006644	25.76435453015439	24.325163605414794
26	16.86506339110799	30.440191534306337	27.157540417342364	25.5372046572433
27	17.919920564635163	26.533980470861557	35.80802097829712	19.738077986206157
28	16.09812107759133	24.095905168282595	33.3998489501267	26.40612480399937
29	18.936440206869317	27.212171399434904	28.21718978228116	25.634198611414615
30	28.510855271985612	28.536924793264863	20.450197629973815	22.502022304775707
31	31.242787751925505	26.126452513216865	20.825713748988846	21.805045985868787
32	25.882817501849786	28.047546206309594	21.230749767099496	24.838886524741124
33	18.886984791501334	30.151509923669977	24.992044962256703	25.969460322571987
34	19.72561828853593	27.810045199949396	28.18191925349159	24.282417258023088
35	31.943981199274653	22.82789132076629	23.516433382788748	21.711694097170305
36	21.88191273611127	32.224611928339485	24.65985025360277	21.23362508194647
37	21.721853542962954	31.8395114265012	23.087628095276433	23.35100693525941
38	21.172285031877657	30.608493296682653	24.03283992930559	24.186381742134095
39	26.93844142600281	25.31657216465203	22.62623590616506	25.1187505031801
40	16.733373971116507	23.08072733964369	28.38798348419152	31.797915205048287
41	22.54246840028983	27.18744369175091	21.123979742448466	29.146108165510793
42	23.064433888844164	23.666524817800884	25.33746611920672	27.931575174148236
43	22.664765125114535	28.010933863924762	26.10460012037985	23.219700890580853
44	18.124642981739832	33.29288723781921	22.38758477386607	26.194885006574886
45	23.015745224102037	29.05198952618645	24.39340441111635	23.53886083859516
46	23.09357041262685	25.151337404779156	25.64646662142838	26.108625561165617
47	22.87715504847781	20.541632642107643	31.743667598268676	24.83754471114587
48	21.066473445508947	20.266369167423832	29.08879355622774	29.57836383083948
49	19.874751285265738	27.14182202951223	28.87084469082698	24.11258199439505
50	18.1796573391453	30.629003875924415	25.867099114019652	25.324239670910632
51	21.42147898528222	23.45873539819277	27.739504142370258	27.380281474154756
52	24.646048742337285	21.37815757492112	29.928193803888192	24.0475998788534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	91.0
2	143.0
3	569.5
4	996.0
5	738.5
6	481.0
7	513.5
8	546.0
9	609.0
10	672.0
11	676.0
12	680.0
13	642.0
14	580.5
15	557.0
16	550.0
17	543.0
18	507.0
19	471.0
20	525.5
21	580.0
22	722.0
23	864.0
24	1056.0
25	1248.0
26	1180.0
27	1112.0
28	1573.5
29	2035.0
30	2701.5
31	3368.0
32	3849.5
33	4331.0
34	4988.0
35	5645.0
36	6833.0
37	8021.0
38	9094.5
39	12078.5
40	13989.0
41	21573.0
42	29157.0
43	38761.0
44	48365.0
45	54291.5
46	60218.0
47	60489.5
48	60761.0
49	59443.0
50	58125.0
51	51138.0
52	44151.0
53	40114.5
54	36078.0
55	31640.5
56	27203.0
57	24670.5
58	22138.0
59	20968.0
60	19798.0
61	19044.0
62	18290.0
63	16974.0
64	12480.0
65	9302.0
66	7836.5
67	6371.0
68	4877.0
69	3383.0
70	2691.5
71	2000.0
72	1631.5
73	1263.0
74	1145.0
75	1027.0
76	941.0
77	855.0
78	547.0
79	239.0
80	185.0
81	131.0
82	75.5
83	20.0
84	25.5
85	31.0
86	27.0
87	23.0
88	12.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	521682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.749715344023397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42880934240276	17.678426985869873
2	11.524762128321262	5.7046916416706495
3	4.1122458629762555	3.0533074359990047
4	1.9772434714202112	1.9574485233391554
5	1.0818098918511532	1.3387243439832388
6	0.6742324713056828	1.0012237044307843
7	0.43970135155989637	0.7617738301242855
8	0.3907878958465653	0.7737511345673883
9	0.3473517047397654	0.773717023491341
>10	7.241529195927759	49.236806646434374
>50	0.6528228845557696	10.352108990534308
>100	0.11939991000188661	4.7654088135042825
>500	0.005427268636449391	0.835299370427001
>1k	0.0038766204546067076	1.767311555624323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2752	0.5275244305918165	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	2026	0.38835919199819047	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	1791	0.3433125927289038	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1607	0.3080420639393347	No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG	1034	0.19820503678486126	Illumina Single End Adapter 1 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	822	0.15756725361427076	Illumina Single End Adapter 1 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	673	0.12900579280097837	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	623	0.1194214099777259	Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	608	0.11654609513075014	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTGGACTTAGGCATTTAGATGCTTGAGCCTATGAGGGCCATTTTTATTC	560	0.10734508762042777	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	558	0.10696171230749768	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3973685118520478	0.0
2	0.0	0.0	0.0	1.4886463401075751	0.0
3	0.0	0.0	0.0	2.3527742954520185	0.0
4	0.0	0.0	0.0	2.8580629578938894	0.0
5	0.0	0.0	0.0	5.517920879002918	0.0
6	0.0	0.0	0.0	5.903021380841202	0.0
7	0.0	0.0	0.0	7.300999459440809	0.0
8	0.0	0.0	0.0	8.920951844226943	0.0
9	0.0	0.0	0.0	9.950122871787794	0.0
10	0.0	0.0	0.0	10.854121859676967	0.0
11	0.0	0.0	0.0	11.67761203185082	0.0
12	0.0	0.0	0.0	12.456822355381247	0.0
13	0.0	0.0	0.0	12.765439482289977	0.0
14	0.0	0.0	0.0	13.000256861459663	0.0
15	0.0	0.0	0.0	13.79537726047669	0.0
16	0.0	0.0	0.0	14.684041235848659	0.0
17	0.0	0.0	0.0	15.807330902733849	0.0
18	0.0	0.0	0.0	16.33274676910455	0.0
19	0.0	0.0	0.0	16.986976740619763	0.0
20	0.0	0.0	0.0	17.769637441966562	0.0
21	0.0	0.0	0.0	18.3776706882737	0.0
22	0.0	0.0	0.0	19.15822282539938	0.0
23	0.0	0.0	0.0	19.678846500358457	0.0
24	0.0	0.0	0.0	20.117619546006953	0.0
25	0.0	0.0	0.0	20.582270425278235	0.0
26	0.0	0.0	0.0	20.944176720684247	0.0
27	0.0	0.0	0.0	21.379882763829308	0.0
28	0.0	0.0	0.0	21.76536664098052	0.0
29	0.0	0.0	0.0	22.293274446885267	0.0
30	0.0	0.0	0.0	22.807955804493925	0.0
31	0.0	0.0	0.0	23.199765374308488	0.0
32	0.0	0.0	0.0	23.593300133031235	0.0
33	0.0	0.0	0.0	24.018271667414247	0.0
34	0.0	0.0	0.0	24.597743452908094	0.0
35	0.0	0.0	0.0	24.99127821163084	0.0
36	0.0	0.0	0.0	25.411265866945765	0.0
37	0.0	0.0	0.0	25.804033875042652	0.0
38	0.0	0.0	0.0	26.197185258452468	0.0
39	0.0	0.0	0.0	26.64190062145138	0.0
40	0.0	0.0	0.0	27.063613465674493	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	20	6.3082675E-4	46.000004	45
CGGACAA	20	6.3082675E-4	46.000004	34
AAGTAGT	20	6.3082675E-4	46.000004	13
TTAGGCA	55	1.8189894E-12	46.000004	10
CGAACTC	20	6.3082675E-4	46.000004	12
TCCGCTT	20	6.3082675E-4	46.000004	12
GTCGAAC	20	6.3082675E-4	46.000004	10
TAGACCC	20	6.3082675E-4	46.000004	13
GGGTCGT	20	6.3082675E-4	46.000004	28
AGCTTAC	20	6.3082675E-4	46.000004	10
GACCGGC	20	6.3082675E-4	46.000004	33
GGCAACC	20	6.3082675E-4	46.000004	7
ATTCGTC	20	6.3082675E-4	46.000004	33
TTCGTCT	20	6.3082675E-4	46.000004	34
AGGGTAC	20	6.3082675E-4	46.000004	5
GTAGCGA	20	6.3082675E-4	46.000004	28
CTAACGG	70	0.0	46.000004	1
AGTGCCC	20	6.3082675E-4	46.000004	8
TGACCGT	20	6.3082675E-4	46.000004	18
CCATCGA	20	6.3082675E-4	46.000004	11
>>END_MODULE