FastQCFastQC Report
Thu 26 May 2016
SRR1544832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544832_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1583522
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT58120.3670299496944153Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT47990.3030586250143667Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT35370.2233628582362607Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA34520.2179950767971648No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.18761974888886923No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23950.15124513584276064No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT16830.1062820724940986No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC16090.10160894512359157No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG206.312179E-446.00000426
CGTTAGT206.312179E-446.00000417
CACTACG206.312179E-446.00000413
CGGTCTA800.046.00000430
TAGTCCG206.312179E-446.00000432
CTCGCTA253.4177454E-546.022
TCATACG301.8618521E-646.031
TATCGTG253.4177454E-546.045
ATCGATT253.4177454E-546.014
CGAAGTA301.8618521E-646.033
AATACGG950.045.9999961
CTAGCGG4950.044.606061
CATGCGG2150.043.8604661
CGACGGT850.043.2941227
ATTGCGG3900.043.051281
TACGGGT601.8189894E-1242.1666683
CTAGTGG1500.041.41
CCGTAGG508.731149E-1041.3999981
ATCAACG10400.041.3557714
CGTCATA7450.041.06040637
Produced by FastQC (version 0.11.5)