##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544832_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1583522
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92841716123932	34.0	33.0	34.0	31.0	34.0
2	33.26524165752039	34.0	33.0	34.0	31.0	34.0
3	33.33042736381307	34.0	34.0	34.0	31.0	34.0
4	36.60398845106036	37.0	37.0	37.0	35.0	37.0
5	36.5997333791384	37.0	37.0	37.0	35.0	37.0
6	36.684477386484055	37.0	37.0	37.0	35.0	37.0
7	36.656880674850115	37.0	37.0	37.0	35.0	37.0
8	36.54860620818656	37.0	37.0	37.0	35.0	37.0
9	38.35830698910404	39.0	39.0	39.0	37.0	39.0
10	38.15965929112447	39.0	39.0	39.0	35.0	39.0
11	37.54844580624709	39.0	35.0	39.0	35.0	39.0
12	37.48098542363162	39.0	35.0	39.0	35.0	39.0
13	37.46726853179179	39.0	35.0	39.0	35.0	39.0
14	38.77620835075231	41.0	35.0	41.0	35.0	41.0
15	38.82036372087031	41.0	35.0	41.0	35.0	41.0
16	38.79515725073602	41.0	35.0	41.0	35.0	41.0
17	38.84883064460109	41.0	36.0	41.0	35.0	41.0
18	38.83054545500473	41.0	36.0	41.0	35.0	41.0
19	38.77682532986596	41.0	36.0	41.0	35.0	41.0
20	38.70371867268027	41.0	35.0	41.0	35.0	41.0
21	38.645986604543545	41.0	35.0	41.0	35.0	41.0
22	38.62035639542741	41.0	35.0	41.0	35.0	41.0
23	38.58035379363217	41.0	35.0	41.0	35.0	41.0
24	38.5262882359702	41.0	35.0	41.0	35.0	41.0
25	38.47033511375276	40.0	35.0	41.0	35.0	41.0
26	38.384426613586676	40.0	35.0	41.0	35.0	41.0
27	38.36168553389217	40.0	35.0	41.0	35.0	41.0
28	38.38852696710244	40.0	35.0	41.0	35.0	41.0
29	38.359319289533076	40.0	36.0	41.0	35.0	41.0
30	38.32374479167324	40.0	35.0	41.0	35.0	41.0
31	38.244237212997355	40.0	35.0	41.0	35.0	41.0
32	38.172903186693965	40.0	35.0	41.0	35.0	41.0
33	38.075433116811766	40.0	35.0	41.0	35.0	41.0
34	37.950675772108	40.0	35.0	41.0	35.0	41.0
35	37.8944567868334	40.0	35.0	41.0	35.0	41.0
36	37.83040147216142	40.0	35.0	41.0	35.0	41.0
37	37.75123490548284	40.0	35.0	41.0	35.0	41.0
38	37.70066724680807	40.0	35.0	41.0	35.0	41.0
39	37.56873854610166	40.0	35.0	41.0	34.0	41.0
40	37.472833973888584	39.0	35.0	41.0	34.0	41.0
41	37.413615346045084	39.0	35.0	41.0	34.0	41.0
42	37.36202844040058	39.0	35.0	41.0	34.0	41.0
43	37.37001317316715	39.0	35.0	41.0	34.0	41.0
44	37.336366024595804	39.0	35.0	41.0	34.0	41.0
45	37.31440864099141	39.0	35.0	41.0	34.0	41.0
46	37.268901221454456	39.0	35.0	41.0	34.0	41.0
47	37.189949997537134	39.0	35.0	41.0	34.0	41.0
48	37.13582760454228	38.0	35.0	41.0	34.0	41.0
49	37.07148495568739	38.0	35.0	41.0	34.0	41.0
50	36.999456906819105	38.0	35.0	41.0	34.0	41.0
51	36.909489100877664	38.0	35.0	41.0	34.0	41.0
52	36.63860306329814	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	3.0
13	6.0
14	5.0
15	19.0
16	28.0
17	53.0
18	101.0
19	177.0
20	376.0
21	649.0
22	1018.0
23	1752.0
24	2749.0
25	4489.0
26	6444.0
27	7809.0
28	8310.0
29	9151.0
30	10576.0
31	13209.0
32	16842.0
33	24252.0
34	80828.0
35	295019.0
36	64140.0
37	99467.0
38	173918.0
39	755518.0
40	6610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.68724905621772	22.07768505900139	23.908477431952317	20.326588452828567
2	27.573724899306733	24.770290529591634	25.7661718624686	21.889812708633034
3	27.5441073758369	24.890402533087634	24.844744815670385	22.720745275405076
4	26.851410968714045	26.34305049124673	22.62917723909109	24.17636130094814
5	25.87769541566205	35.24725264315873	19.840772657405452	19.034279283773763
6	87.95703501435408	3.2012185495370447	6.432054622543924	2.4096918135649523
7	88.6359646408449	3.181326183027454	4.7137330583345225	3.468976117793122
8	79.32816847508276	5.819180282938917	9.125228446463012	5.727422795515313
9	51.0150158949481	22.667383212863477	13.1015546358055	13.216046256382924
10	40.44358082805291	23.132359386228927	22.166095576821792	14.25796420889637
11	40.54329526208035	18.269654605367023	23.041549154353397	18.14550097819923
12	23.614070407610377	30.762818577828412	25.473469898113194	20.14964111644802
13	16.998121908000016	33.16044867074787	27.78275262358212	22.058676797670003
14	16.25736806940478	25.55373401822014	38.6076101247725	19.581287787602573
15	19.145802836967217	18.638515915787718	41.86440099979666	20.351280247448408
16	19.420191194059825	21.358149744683054	27.54050780475421	31.68115125650291
17	21.771026862904336	24.485608662209934	28.447473416851803	25.295891058033927
18	25.554491822658605	27.687206113966212	25.33030800961401	21.427994053761175
19	30.5880183540235	23.308233166321653	24.077846723948262	22.025901755706585
20	24.800665857499926	27.600058603543243	25.540030387957984	22.059245150998848
21	22.45260880492977	24.749829809753194	25.336054693272338	27.461506692044697
22	22.14456130069554	27.502680733201057	24.13487150794242	26.217886458160983
23	22.944044983271468	22.10269260547059	33.431932110826374	21.521330300431572
24	21.82931465429593	21.823504820267733	30.437846774468557	25.90933375096778
25	20.03957002176162	29.960935181197357	26.482802259772836	23.51669253726819
26	19.53348295760968	29.438807923098004	26.889238040267198	24.13847107902511
27	21.66619724891729	27.466748172744047	31.545188510169105	19.32186606816956
28	20.08352267919233	25.751205224809006	29.598073155914474	24.567198940084193
29	21.93193400533747	27.44489814476843	28.064529573949713	22.558638275944382
30	28.188556900377765	25.166622250906524	23.612049595774483	23.032771252941227
31	27.872741900649313	26.44251232379468	25.275746090044848	20.40899968551116
32	27.1705729380457	26.99956173643309	25.61808424511942	20.21178108040179
33	23.469266609494532	25.61056935110469	26.82804533186151	24.09211870753927
34	21.979612534590615	24.81512729220055	30.110980460012556	23.09427971319628
35	28.662689877374614	24.274812727578148	24.884087496100467	22.17840989894678
36	25.292922990649956	28.488015954309443	26.142737518013643	20.07632353702696
37	24.44569762845101	26.31558008035253	26.906667542351798	22.332054748844666
38	24.21879834950193	25.994144697705497	25.912175517612006	23.874881435180566
39	28.252528225057816	23.233399978023673	27.115253214038077	21.39881858288044
40	19.973577885245675	22.587434844605887	30.81472818186296	26.62425908828548
41	22.83302663303699	25.858055650631943	24.521730673776556	26.787187042554507
42	23.782492444058246	23.29257187459347	27.546507089892025	25.37842859145626
43	23.737908282928814	26.432660866094693	27.88916099681596	21.940269854160537
44	21.118936143608995	27.633275698095765	26.358711782974915	24.88907637532033
45	22.915248414609966	26.34241898754801	26.13446481956045	24.607867778281577
46	24.056691350041238	24.17598239872891	28.432443628822334	23.33488262240752
47	24.244247948560236	20.858946070847136	31.74007055159322	23.156735428999408
48	21.589785301372512	21.8380925557081	29.45522701926465	27.11689512365474
49	20.73807626291267	24.875562196167785	31.110903416561307	23.275458124358234
50	20.52595417051358	25.9483606795485	28.13860495780924	25.387080192128685
51	22.535777842050823	22.6559529959167	29.071209620074747	25.737059541957734
52	24.239511670819855	20.62244793567756	30.251868935196352	24.88617145830623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	549.5
2	878.0
3	3401.0
4	5924.0
5	3965.0
6	2006.0
7	2037.0
8	2068.0
9	2095.0
10	2122.0
11	2073.5
12	2025.0
13	1912.5
14	1794.5
15	1789.0
16	1826.0
17	1863.0
18	1892.5
19	1922.0
20	2179.5
21	2437.0
22	2946.0
23	3455.0
24	4133.0
25	4811.0
26	5576.5
27	6342.0
28	9782.5
29	13223.0
30	14144.5
31	15066.0
32	16999.5
33	18933.0
34	24342.5
35	29752.0
36	31591.5
37	33431.0
38	38376.0
39	46164.5
40	49008.0
41	61456.0
42	73904.0
43	95457.0
44	117010.0
45	126637.0
46	136264.0
47	141506.0
48	146748.0
49	147490.5
50	148233.0
51	137499.0
52	126765.0
53	115432.5
54	104100.0
55	95068.5
56	86037.0
57	80110.0
58	74183.0
59	75068.5
60	75954.0
61	78791.5
62	81629.0
63	73670.0
64	52610.0
65	39509.0
66	31270.0
67	23031.0
68	18955.0
69	14879.0
70	12583.0
71	10287.0
72	8450.5
73	6614.0
74	5457.0
75	4300.0
76	4256.5
77	4213.0
78	2702.0
79	1191.0
80	785.5
81	380.0
82	256.0
83	132.0
84	74.0
85	16.0
86	12.5
87	9.0
88	12.0
89	10.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1583522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.501122126764486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.59433894267791	14.472631641220456
2	12.073382879165727	4.950357937779281
3	4.476970319712971	2.7534874584700644
4	2.1517062011050188	1.7644956643908183
5	1.2515180737991298	1.2828762437404506
6	0.7682444825437398	0.9449924375905319
7	0.5480574777508671	0.7865055298699927
8	0.4032362199345513	0.661343599265048
9	0.3282814692759645	0.605712464422221
>10	4.677398954015216	25.606634769496743
>50	2.4345695909952414	33.66318919249176
>100	0.2790372648387732	8.877071356780867
>500	0.00616656938833842	0.8437377058989344
>1k	0.006783226327172261	2.4195828518429283
>5k	3.0832846941692094E-4	0.36738114673993155
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	5812	0.3670299496944153	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	4799	0.3030586250143667	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3537	0.2233628582362607	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	3452	0.2179950767971648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2971	0.18761974888886923	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2395	0.15124513584276064	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1683	0.1062820724940986	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1609	0.10160894512359157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3098788649605121	0.0
2	0.0	0.0	0.0	1.0573897931320184	0.0
3	0.0	0.0	0.0	1.639383601869756	0.0
4	0.0	0.0	0.0	1.9870263880135546	0.0
5	0.0	0.0	0.0	3.8286174742125465	0.0
6	0.0	0.0	0.0	4.118161920074366	0.0
7	0.0	0.0	0.0	5.099202916031479	0.0
8	0.0	0.0	0.0	6.316299994569068	0.0
9	0.0	0.0	0.0	7.1373179532712525	0.0
10	0.0	0.0	0.0	7.85836887646651	0.0
11	0.0	0.0	0.0	8.54127697625925	0.0
12	0.0	0.0	0.0	9.14834148183606	0.0
13	0.0	0.0	0.0	9.429676379614556	0.0
14	0.0	0.0	0.0	9.637504246862374	0.0
15	0.0	0.0	0.0	10.301719837173087	0.0
16	0.0	0.0	0.0	11.012224648599767	0.0
17	0.0	0.0	0.0	11.880226482486508	0.0
18	0.0	0.0	0.0	12.350002083962206	0.0
19	0.0	0.0	0.0	12.846427141523767	0.0
20	0.0	0.0	0.0	13.46757417958197	0.0
21	0.0	0.0	0.0	14.041484740976127	0.0
22	0.0	0.0	0.0	14.658021802033694	0.0
23	0.0	0.0	0.0	15.141627334511298	0.0
24	0.0	0.0	0.0	15.568397534104358	0.0
25	0.0	0.0	0.0	15.955067248828877	0.0
26	0.0	0.0	0.0	16.321970897783547	0.0
27	0.0	0.0	0.0	16.70169407182218	0.0
28	0.0	0.0	0.0	17.077565073298633	0.0
29	0.0	0.0	0.0	17.549614088089715	0.0
30	0.0	0.0	0.0	18.015853268852595	0.0
31	6.315036987171633E-5	0.0	0.0	18.45973721868089	0.0
32	6.315036987171633E-5	0.0	0.0	18.872298585052814	0.0
33	6.315036987171633E-5	0.0	0.0	19.259915555325406	0.0
34	6.315036987171633E-5	0.0	0.0	19.752488440324797	0.0
35	6.315036987171633E-5	0.0	0.0	20.1740171592185	0.0
36	1.2630073974343267E-4	0.0	0.0	20.65661228577816	0.0
37	1.2630073974343267E-4	0.0	0.0	21.08723465793339	0.0
38	1.2630073974343267E-4	0.0	0.0	21.516025669362346	0.0
39	1.2630073974343267E-4	0.0	0.0	21.97102408428806	0.0
40	1.2630073974343267E-4	0.0	0.0	22.44749362497016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCG	20	6.312179E-4	46.000004	26
CGTTAGT	20	6.312179E-4	46.000004	17
CACTACG	20	6.312179E-4	46.000004	13
CGGTCTA	80	0.0	46.000004	30
TAGTCCG	20	6.312179E-4	46.000004	32
CTCGCTA	25	3.4177454E-5	46.0	22
TCATACG	30	1.8618521E-6	46.0	31
TATCGTG	25	3.4177454E-5	46.0	45
ATCGATT	25	3.4177454E-5	46.0	14
CGAAGTA	30	1.8618521E-6	46.0	33
AATACGG	95	0.0	45.999996	1
CTAGCGG	495	0.0	44.60606	1
CATGCGG	215	0.0	43.860466	1
CGACGGT	85	0.0	43.29412	27
ATTGCGG	390	0.0	43.05128	1
TACGGGT	60	1.8189894E-12	42.166668	3
CTAGTGG	150	0.0	41.4	1
CCGTAGG	50	8.731149E-10	41.399998	1
ATCAACG	1040	0.0	41.35577	14
CGTCATA	745	0.0	41.060406	37
>>END_MODULE