SRR1544831_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544831_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1152297
Sequences flagged as poor quality	0
Sequence length	52
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	6407	0.5560198455780063	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	5062	0.43929646610205525	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4132	0.35858810705920435	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3936	0.3415786034329691	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTTG	2628	0.22806620168237876	Illumina Single End Adapter 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1901	0.16497482853812862	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1707	0.14813889127542637	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1466	0.12722414446969835	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1431	0.12418673310787064	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1403	0.12175680401840844	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1316	0.1142066672047224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1272	0.11038820720699612	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1184	0.10275128721154356	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1156	0.10032135812208136	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGT	20	6.3114613E-4	46.0	45
ACGCATA	20	6.3114613E-4	46.0	25
GCGCGAC	30	1.8614082E-6	46.0	8
TAACGTT	20	6.3114613E-4	46.0	23
CGGTCTA	60	0.0	46.0	30
CCGCGAA	30	1.8614082E-6	46.0	13
AATACGG	50	1.6370905E-11	46.0	1
CCGCAAT	20	6.3114613E-4	46.0	32
CCGTAGG	45	3.092282E-10	45.999996	1
TCATACG	45	3.092282E-10	45.999996	31
CTAGTGG	120	0.0	44.083332	1
TACGGGT	95	0.0	43.57895	3
ATAAGCG	90	0.0	43.444443	32
TCGGCGT	590	0.0	43.271183	4
CTAGCGG	335	0.0	43.253735	1
CCAACGG	75	0.0	42.933334	1
GCATAGG	200	0.0	42.550003	1
CGGGATC	185	0.0	42.27027	5
TCAAGCG	305	0.0	42.229507	16
GTCGGCG	605	0.0	42.19835	3