##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544831_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1152297
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.933503254803234	34.0	33.0	34.0	31.0	34.0
2	33.281211354364366	34.0	34.0	34.0	31.0	34.0
3	33.38420997364395	34.0	34.0	34.0	31.0	34.0
4	36.609118135341845	37.0	37.0	37.0	35.0	37.0
5	36.60901833468281	37.0	37.0	37.0	35.0	37.0
6	36.538283966720385	37.0	37.0	37.0	35.0	37.0
7	36.4192157056731	37.0	37.0	37.0	35.0	37.0
8	36.44651335549776	37.0	37.0	37.0	35.0	37.0
9	38.304111700368914	39.0	39.0	39.0	37.0	39.0
10	38.17498526855489	39.0	39.0	39.0	35.0	39.0
11	37.62830242550315	39.0	35.0	39.0	35.0	39.0
12	37.5998653125019	39.0	35.0	39.0	35.0	39.0
13	37.59019593038948	39.0	35.0	39.0	35.0	39.0
14	38.87185855729903	41.0	36.0	41.0	35.0	41.0
15	38.91279765546556	41.0	36.0	41.0	35.0	41.0
16	38.89890019673747	41.0	36.0	41.0	35.0	41.0
17	38.9649526120436	41.0	36.0	41.0	35.0	41.0
18	38.95973867848306	41.0	36.0	41.0	35.0	41.0
19	38.91539420826402	41.0	36.0	41.0	35.0	41.0
20	38.85919255192021	41.0	35.0	41.0	35.0	41.0
21	38.79993959890549	41.0	35.0	41.0	35.0	41.0
22	38.78465708059641	41.0	35.0	41.0	35.0	41.0
23	38.75618698998609	41.0	35.0	41.0	35.0	41.0
24	38.68334465853855	41.0	35.0	41.0	35.0	41.0
25	38.621073386461994	41.0	35.0	41.0	35.0	41.0
26	38.522600510111545	40.0	35.0	41.0	35.0	41.0
27	38.51937998623619	40.0	36.0	41.0	35.0	41.0
28	38.56243746186964	40.0	36.0	41.0	35.0	41.0
29	38.565854983567604	40.0	36.0	41.0	35.0	41.0
30	38.55808094614496	40.0	35.0	41.0	35.0	41.0
31	38.47814582525165	40.0	35.0	41.0	35.0	41.0
32	38.45725277424136	40.0	35.0	41.0	35.0	41.0
33	38.38902123324108	40.0	35.0	41.0	35.0	41.0
34	38.26768966681333	40.0	35.0	41.0	35.0	41.0
35	38.219478138014765	40.0	35.0	41.0	35.0	41.0
36	38.20386931494224	40.0	35.0	41.0	35.0	41.0
37	38.127629421928546	40.0	35.0	41.0	35.0	41.0
38	38.066755359078435	40.0	35.0	41.0	35.0	41.0
39	38.02279099919552	40.0	35.0	41.0	35.0	41.0
40	37.96437029689395	40.0	35.0	41.0	35.0	41.0
41	37.91544888166853	40.0	35.0	41.0	35.0	41.0
42	37.87040841033171	40.0	35.0	41.0	35.0	41.0
43	37.798491187601805	40.0	35.0	41.0	35.0	41.0
44	37.66745118662983	39.0	35.0	41.0	35.0	41.0
45	37.649351686240614	39.0	35.0	41.0	35.0	41.0
46	37.602339501014065	39.0	35.0	41.0	35.0	41.0
47	37.532302002001224	39.0	35.0	41.0	35.0	41.0
48	37.452368616771544	39.0	35.0	41.0	35.0	41.0
49	37.40876180359751	39.0	35.0	41.0	35.0	41.0
50	37.34639854134828	39.0	35.0	41.0	35.0	41.0
51	37.267427581604395	39.0	35.0	41.0	34.0	41.0
52	36.82178119009249	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	2.0
14	11.0
15	15.0
16	32.0
17	58.0
18	93.0
19	156.0
20	277.0
21	407.0
22	606.0
23	994.0
24	1512.0
25	2517.0
26	3546.0
27	4405.0
28	4592.0
29	4994.0
30	6134.0
31	7610.0
32	10182.0
33	14932.0
34	52227.0
35	207267.0
36	43948.0
37	69933.0
38	126111.0
39	586373.0
40	3358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.69678129857146	21.825970214276357	23.188205818465203	21.28904266868698
2	27.491436669539187	24.763841266617895	25.504275373449726	22.24044669039319
3	27.908603424290785	24.562417501737833	24.583158682180027	22.94582039179135
4	26.77955422950854	25.893758293217807	22.812434641416232	24.514252835857423
5	25.57404905159	34.69322579161449	19.86467030635331	19.868054850442203
6	85.90363421930284	3.2903843366770893	7.454241397834066	3.3517400461860096
7	86.93218848959947	3.25558428078872	5.452500527207829	4.3597267024039805
8	76.33361885000134	5.953152702818804	10.670426114100792	7.042802333079059
9	49.55024616049508	21.89695885696136	13.956905207598389	14.595889774945176
10	38.85612823777203	22.41748438119686	24.06072392794566	14.665663453085445
11	38.559416539312345	18.15391344419017	23.64737563319179	19.639294383305693
12	22.93471214452524	29.34547256479883	26.824855050390656	20.894960240285272
13	16.786470849095327	31.954001442336484	28.765847693780337	22.493680014787852
14	16.109475248134814	25.439361553488382	37.63656418440732	20.814599013969488
15	18.689973157961877	18.791162347901626	41.38125847763207	21.137606016504424
16	18.866143016947888	22.40984746120141	27.410728310496342	31.313281211354365
17	20.945381268891612	24.605375176712254	28.591239932066127	25.858003622330006
18	24.661610678496952	27.814964371164724	25.75039247693954	21.773032473398786
19	29.177807457625942	23.194454207552393	24.404211761377493	23.223526573444172
20	23.61257557730342	28.375670508558127	25.899746332759698	22.11200758137876
21	21.825015599276924	24.58732427490482	25.67228761334968	27.915372512468572
22	21.469291337216013	27.582211877666957	24.223702743303157	26.724794041813873
23	21.877172291518594	22.169197698162886	33.876943183918726	22.07668682639979
24	22.117822054557116	22.135959739546315	29.86426242539901	25.881955780497563
25	19.927154197225192	29.615888959183266	26.282720513895292	24.17423632969625
26	19.08674586499835	28.870768560536042	26.693552096377932	25.34893347808768
27	22.73649935737054	26.686435875473073	30.918851650225594	19.658213116930792
28	20.337725430162536	25.33452746991444	29.08269309040985	25.245054009513172
29	21.914749409223493	27.422010124125983	27.7922271775419	22.871013289108625
30	28.755433711968355	24.895751702902984	23.214935038449287	23.133879546679374
31	27.709696371681954	25.519202080713566	25.69823578469787	21.072865762906613
32	26.95511660622218	26.210690473029093	26.280203801624058	20.55398911912467
33	24.33105353914833	24.54627582992926	26.935850739870016	24.186819891052394
34	22.42468738528348	23.542888682344916	31.316578972261492	22.71584496011011
35	29.13432908356092	23.45662619966901	25.51000306344632	21.899041653323753
36	26.364557054301102	27.38382552414872	26.08337954537762	20.168237876172547
37	24.925431551067128	25.532913823432672	27.931427401095377	21.610227224404817
38	24.561896802647233	25.166602013196247	25.68981781606652	24.581683368089998
39	29.277781683020958	22.053689283231666	27.17719476836267	21.491334265384708
40	20.62350244771964	21.559111930344347	30.94028709612192	26.87709852581409
41	23.01351127356923	25.502105793905567	24.527877795394765	26.956505137130442
42	23.471118991024014	22.098556188204952	28.385650574461273	26.04467424630976
43	24.787446292058384	25.14152167366573	27.818696047980684	22.2523359862952
44	21.44429778086726	26.128246450350908	27.04502398253228	25.382431786249555
45	22.88993202273372	24.963269018317327	26.596528499162975	25.550270459785974
46	24.731557923000754	22.59113752791164	28.72254288607885	23.954761663008757
47	23.61656760366468	20.012809197628737	32.953656913104865	23.416966285601713
48	21.68564180935991	20.45262636282139	29.5266758483273	28.335055979491397
49	21.05629017518921	23.131362834408144	31.868433225114707	23.94391376528794
50	20.878211086204338	24.002405629798567	29.32143362344951	25.797949660547587
51	22.599468713361226	21.140643427866255	29.341220188892276	26.918667669880247
52	24.01906800069774	19.657171718749595	31.810375276512914	24.513385004039755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	290.0
2	443.0
3	1639.0
4	2835.0
5	1959.0
6	1083.0
7	1112.5
8	1142.0
9	1150.0
10	1158.0
11	1148.0
12	1138.0
13	1085.0
14	1033.5
15	1035.0
16	1061.5
17	1088.0
18	1160.0
19	1232.0
20	1395.5
21	1559.0
22	1858.5
23	2158.0
24	2644.5
25	3131.0
26	3659.5
27	4188.0
28	6170.5
29	8153.0
30	8906.5
31	9660.0
32	10826.5
33	11993.0
34	15660.5
35	19328.0
36	20661.0
37	21994.0
38	25572.0
39	31243.5
40	33337.0
41	41882.0
42	50427.0
43	65624.0
44	80821.0
45	89577.5
46	98334.0
47	108522.5
48	118711.0
49	121311.0
50	123911.0
51	112569.5
52	101228.0
53	90270.5
54	79313.0
55	70943.5
56	62574.0
57	57576.5
58	52579.0
59	52418.0
60	52257.0
61	53591.5
62	54926.0
63	51406.0
64	37600.0
65	27314.0
66	21613.5
67	15913.0
68	13018.0
69	10123.0
70	8649.0
71	7175.0
72	5891.0
73	4607.0
74	3766.0
75	2925.0
76	3012.0
77	3099.0
78	1951.0
79	803.0
80	549.0
81	295.0
82	179.5
83	64.0
84	38.5
85	13.0
86	11.5
87	10.0
88	10.0
89	6.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1152297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.78351084704523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.45646208851676	16.757020303266692
2	11.881477484348167	5.651664972558366
3	4.298109468058269	3.066723994660549
4	2.087256329033787	1.985691341685555
5	1.1923215241060392	1.4178795950870742
6	0.7437520403916782	1.06134208321005
7	0.5412459948194993	0.9010910992096545
8	0.4265802824463345	0.8116461419758411
9	0.3525539491886584	0.754647360722738
>10	7.080095911949	45.67867599007885
>50	0.7863829327890868	11.696916734096092
>100	0.13875189490398956	5.521634734281626
>500	0.009884699636958946	1.6474785272780554
>1k	0.004393199838648421	2.048964891068506
>5k	7.321999731080701E-4	0.9986222308203593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	6407	0.5560198455780063	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	5062	0.43929646610205525	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4132	0.35858810705920435	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3936	0.3415786034329691	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTTG	2628	0.22806620168237876	Illumina Single End Adapter 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1901	0.16497482853812862	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1707	0.14813889127542637	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1466	0.12722414446969835	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1431	0.12418673310787064	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1403	0.12175680401840844	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1316	0.1142066672047224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1272	0.11038820720699612	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1184	0.10275128721154356	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1156	0.10032135812208136	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4478012179151729	0.0
2	0.0	0.0	0.0	1.571730204973197	0.0
3	0.0	0.0	0.0	2.4048487499316584	0.0
4	0.0	0.0	0.0	2.924593225531265	0.0
5	0.0	0.0	0.0	5.593349631214869	0.0
6	0.0	0.0	0.0	6.010689952329998	0.0
7	0.0	0.0	0.0	7.375615835153611	0.0
8	0.0	0.0	0.0	9.014429439632316	0.0
9	0.0	0.0	0.0	10.169513588944517	0.0
10	0.0	0.0	0.0	11.172119687892964	0.0
11	0.0	0.0	0.0	12.074664778264632	0.0
12	0.0	0.0	0.0	12.888343890507395	0.0
13	0.0	0.0	0.0	13.268974925735293	0.0
14	0.0	0.0	0.0	13.543209780117452	0.0
15	0.0	0.0	0.0	14.3841388114349	0.0
16	0.0	0.0	0.0	15.270802579543295	0.0
17	0.0	0.0	0.0	16.302220694838223	0.0
18	0.0	0.0	0.0	16.883147313583216	0.0
19	0.0	0.0	0.0	17.486290426860435	0.0
20	0.0	0.0	0.0	18.24303977186437	0.0
21	0.0	0.0	0.0	18.951711234169665	0.0
22	0.0	0.0	0.0	19.705249601448237	0.0
23	8.678318176650637E-5	0.0	0.0	20.27558867201772	0.0
24	8.678318176650637E-5	0.0	0.0	20.767822878997343	0.0
25	8.678318176650637E-5	0.0	0.0	21.216578711911946	0.0
26	8.678318176650637E-5	0.0	0.0	21.64867217392738	0.0
27	8.678318176650637E-5	0.0	0.0	22.081633467760483	0.0
28	8.678318176650637E-5	0.0	0.0	22.50669749205283	0.0
29	8.678318176650637E-5	0.0	0.0	23.028698330378365	0.0
30	8.678318176650637E-5	0.0	0.0	23.526747010536347	0.0
31	8.678318176650637E-5	0.0	0.0	24.022973243877228	0.0
32	8.678318176650637E-5	0.0	0.0	24.478237815424322	0.0
33	8.678318176650637E-5	0.0	0.0	24.884730238818637	0.0
34	8.678318176650637E-5	0.0	0.0	25.419401421682085	0.0
35	8.678318176650637E-5	0.0	0.0	25.860259985055936	0.0
36	8.678318176650637E-5	0.0	0.0	26.360738594303378	0.0
37	8.678318176650637E-5	0.0	0.0	26.809494427217984	0.0
38	8.678318176650637E-5	0.0	0.0	27.276214378758254	0.0
39	8.678318176650637E-5	0.0	0.0	27.763588727558954	0.0
40	8.678318176650637E-5	0.0	0.0	28.259901744081603	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGT	20	6.3114613E-4	46.0	45
ACGCATA	20	6.3114613E-4	46.0	25
GCGCGAC	30	1.8614082E-6	46.0	8
TAACGTT	20	6.3114613E-4	46.0	23
CGGTCTA	60	0.0	46.0	30
CCGCGAA	30	1.8614082E-6	46.0	13
AATACGG	50	1.6370905E-11	46.0	1
CCGCAAT	20	6.3114613E-4	46.0	32
CCGTAGG	45	3.092282E-10	45.999996	1
TCATACG	45	3.092282E-10	45.999996	31
CTAGTGG	120	0.0	44.083332	1
TACGGGT	95	0.0	43.57895	3
ATAAGCG	90	0.0	43.444443	32
TCGGCGT	590	0.0	43.271183	4
CTAGCGG	335	0.0	43.253735	1
CCAACGG	75	0.0	42.933334	1
GCATAGG	200	0.0	42.550003	1
CGGGATC	185	0.0	42.27027	5
TCAAGCG	305	0.0	42.229507	16
GTCGGCG	605	0.0	42.19835	3
>>END_MODULE