FastQCFastQC Report
Thu 26 May 2016
SRR1544830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544830_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences527357
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT16330.3096574047561709Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT14980.28405804796371337Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT11450.2171204705730653Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.15928488670862434No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8190.15530276454090872No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT5660.1073276736631921No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC5650.1071380487980628Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT5470.10372480122573513Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA351.0181611E-746.0000044
CTAGCGG1400.046.0000041
TGATCGA206.3083315E-446.044
TCACGAC253.414624E-546.024
GTGCATT206.3083315E-446.012
TTAGGTT206.3083315E-446.039
AACCGTT253.414624E-546.040
GTCGCTA206.3083315E-446.021
CTCACGA253.414624E-546.023
ACCGGGT206.3083315E-446.03
CGTATTA253.414624E-546.034
CTCGTGA301.8594728E-646.039
CGACGGT206.3083315E-446.027
CTATCGG253.414624E-546.01
CCTAGAA253.414624E-546.044
TAGACAA206.3083315E-446.033
TCACACG206.3083315E-446.021
ATAATGG501.6370905E-1146.01
GACGTCA206.3083315E-446.043
GAATCCG206.3083315E-446.035
Produced by FastQC (version 0.11.5)