##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544830_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	527357
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97024216991526	34.0	33.0	34.0	31.0	34.0
2	33.275160849291844	34.0	33.0	34.0	31.0	34.0
3	33.326494196531	34.0	34.0	34.0	31.0	34.0
4	36.593499280373635	37.0	37.0	37.0	35.0	37.0
5	36.58528852371354	37.0	37.0	37.0	35.0	37.0
6	36.69935546508343	37.0	37.0	37.0	36.0	37.0
7	36.68733704113153	37.0	37.0	37.0	36.0	37.0
8	36.609112991768384	37.0	37.0	37.0	35.0	37.0
9	38.40256410742628	39.0	39.0	39.0	37.0	39.0
10	38.10776760335029	39.0	39.0	39.0	35.0	39.0
11	37.055410281839436	39.0	35.0	39.0	35.0	39.0
12	36.90544735350057	39.0	35.0	39.0	35.0	39.0
13	36.88596908735449	39.0	35.0	39.0	35.0	39.0
14	38.03239930445599	40.0	35.0	41.0	35.0	41.0
15	38.13248141202259	40.0	35.0	41.0	35.0	41.0
16	38.14095005849927	40.0	35.0	41.0	35.0	41.0
17	38.25842076619823	40.0	35.0	41.0	35.0	41.0
18	38.245844845142855	40.0	36.0	41.0	35.0	41.0
19	38.11104811351703	40.0	35.0	41.0	35.0	41.0
20	38.00545740361842	40.0	35.0	41.0	35.0	41.0
21	37.95420180257397	40.0	35.0	41.0	35.0	41.0
22	37.94487415545826	40.0	35.0	41.0	35.0	41.0
23	37.900003223622704	40.0	35.0	41.0	35.0	41.0
24	37.86418877534573	40.0	35.0	41.0	35.0	41.0
25	37.83749717933013	40.0	35.0	41.0	35.0	41.0
26	37.781760742722675	39.0	35.0	41.0	35.0	41.0
27	37.800260544564686	39.0	35.0	41.0	35.0	41.0
28	37.810151377529834	39.0	35.0	41.0	35.0	41.0
29	37.83214217313888	39.0	35.0	41.0	35.0	41.0
30	37.71301604036734	39.0	35.0	41.0	35.0	41.0
31	37.63751500406745	39.0	35.0	41.0	35.0	41.0
32	37.55994326424035	39.0	35.0	41.0	35.0	41.0
33	37.486110926753604	39.0	35.0	41.0	35.0	41.0
34	37.407399541487074	39.0	35.0	41.0	35.0	41.0
35	37.331443405510875	39.0	35.0	41.0	35.0	41.0
36	37.253071448752934	38.0	35.0	41.0	34.0	41.0
37	37.20150865542697	38.0	35.0	41.0	34.0	41.0
38	37.18090022508471	38.0	35.0	41.0	35.0	41.0
39	37.050773953887024	38.0	35.0	41.0	34.0	41.0
40	37.015653532616426	38.0	35.0	41.0	34.0	41.0
41	37.004710281649814	38.0	35.0	41.0	34.0	41.0
42	36.89895080562124	37.0	35.0	41.0	34.0	41.0
43	36.88032205887094	37.0	35.0	41.0	34.0	41.0
44	36.837946590260486	37.0	35.0	41.0	34.0	41.0
45	36.8208860411448	37.0	35.0	41.0	34.0	41.0
46	36.76452953122837	37.0	35.0	41.0	34.0	41.0
47	36.71656392159391	36.0	35.0	41.0	34.0	41.0
48	36.74350961492878	36.0	35.0	41.0	34.0	41.0
49	36.65590671973634	36.0	35.0	41.0	34.0	41.0
50	36.55764690712364	36.0	35.0	41.0	34.0	41.0
51	36.46540389148148	36.0	35.0	40.0	34.0	41.0
52	36.23208187243177	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	6.0
16	6.0
17	19.0
18	36.0
19	63.0
20	128.0
21	230.0
22	388.0
23	615.0
24	1072.0
25	1781.0
26	2623.0
27	2954.0
28	2960.0
29	3079.0
30	3567.0
31	4465.0
32	5882.0
33	8666.0
34	30901.0
35	155908.0
36	16584.0
37	23657.0
38	42709.0
39	216629.0
40	2425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.688590461490033	21.713943305957823	25.61585415572373	20.98161207682841
2	24.992936473773934	24.51849506122039	28.003041582836673	22.485526882169005
3	25.395889312173725	25.09855752365096	26.33339464537306	23.172158518802252
4	24.826256217325266	28.208026062041462	24.503514696875172	22.4622030237581
5	22.871792732437417	35.67981462273185	21.36105143195217	20.087341212878563
6	88.79544596923905	2.735717929220623	6.129813390170226	2.3390227113700965
7	90.29044840591857	1.9975083292722007	4.299174942211822	3.4128683225974057
8	82.66695995312473	3.66355239429836	8.597401760097998	5.072085892478909
9	57.70493233236687	18.35246332181046	12.024491947580103	11.918112398242558
10	51.26072091581225	17.505029799547557	20.324751544020465	10.909497740619733
11	48.5248133617265	17.209783884541213	20.268243334211927	13.997159419520363
12	19.396348204347337	44.03316918140842	21.76703826819403	14.80344434605021
13	11.783099494270486	45.48872964614104	25.467946760922867	17.26022409866561
14	9.69286460595005	23.662149170296402	50.52156319153818	16.123423032215367
15	10.814306058324815	15.4754748680685	53.78292124689727	19.92729782670942
16	12.247301164107046	18.92076904260302	27.067432498288635	41.7644972950013
17	18.74555566722353	23.227528979420015	33.69956215618642	24.327353197170037
18	25.996810509768526	25.960402535663697	25.134775872890657	22.908011081677117
19	30.58781811941436	21.502701206203767	24.183807174267148	23.725673500114723
20	18.28647386874546	31.015801440011227	26.62845093551427	24.069273755729043
21	20.244540226070765	25.41731692193334	24.08425412007426	30.25388873192164
22	16.95947906257052	31.607810268944945	21.528300562996225	29.90441010548831
23	17.672847805186997	21.522422192177217	41.5128650989747	19.29186490366109
24	15.628312509362727	22.549999336312972	37.24497825950922	24.57670989481509
25	13.865749387985748	36.22726160836018	27.115407589166352	22.79158141448772
26	15.130167988668017	35.01328322180231	27.018509283085272	22.838039506444403
27	15.874255959435448	28.783158277978675	39.558211989221725	15.784373773364155
28	13.497687524769747	24.391067151853488	36.530471767701954	25.580773555674806
29	15.97665338660528	32.607512557906695	29.859848262182926	21.555985793305105
30	28.47444899754815	29.10836492167545	21.59903063768946	20.81815544308694
31	30.85348255546053	28.400874549877976	22.254563796441502	18.49107909821999
32	25.395130812713205	32.66762364015269	21.73783603896412	20.19940950816999
33	18.194126559427485	29.55151823148266	26.474475545029268	25.77987966406059
34	20.19030751464378	27.06875987234454	28.600739157724277	24.140193455287402
35	31.75078741725245	23.440288078095104	25.07144116793747	19.737483336714977
36	21.152464080309922	29.920907468754564	28.119281625160948	20.80734682577457
37	21.54081580409476	29.619214308333824	25.58778967568459	23.252180211886824
38	20.63573632283254	29.567446720153523	26.35008163350444	23.4467353235095
39	27.004477043065705	24.636441727330823	23.94810346691141	24.410977762692067
40	15.97077501578627	21.513889073246396	31.21926892029498	31.296066990672355
41	23.1983267501901	24.840288457344833	22.448550033468788	29.512834758996277
42	23.401225355878466	25.593478421638473	24.509013817963922	26.49628240451914
43	21.765710894138127	30.544204400434623	26.38516223355336	21.30492247187389
44	16.307359151390806	37.74407090452957	22.519848982757413	23.428720961322217
45	23.217668486433286	31.160105962374633	24.687450816050607	20.93477473514147
46	23.858979780300633	26.34875425944853	25.40783567867687	24.384430281573962
47	23.94013922257598	21.235140521506306	31.08463526605317	23.740084989864553
48	20.09321958369757	19.83931188928942	30.32253293309845	29.744935593914555
49	19.843104386592007	28.720771697351132	29.288129293818038	22.147994622238826
50	18.236033654621064	31.165036208867996	25.30145612933933	25.297474007171612
51	21.324264208117082	22.991825272064276	29.551707856347786	26.132202663470856
52	24.324888073923358	21.17844268683264	29.221381341292517	25.275287897951483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	122.0
2	155.0
3	759.5
4	1364.0
5	988.5
6	613.0
7	730.5
8	848.0
9	869.0
10	890.0
11	877.0
12	864.0
13	836.5
14	844.5
15	880.0
16	783.5
17	687.0
18	744.0
19	801.0
20	779.0
21	757.0
22	878.0
23	999.0
24	978.5
25	958.0
26	1429.5
27	1901.0
28	2745.0
29	3589.0
30	3994.0
31	4399.0
32	4667.5
33	4936.0
34	6800.0
35	8664.0
36	9943.5
37	11223.0
38	12415.0
39	15264.5
40	16922.0
41	25666.0
42	34410.0
43	46279.5
44	58149.0
45	64434.5
46	70720.0
47	67719.5
48	64719.0
49	59754.0
50	54789.0
51	48969.0
52	43149.0
53	37029.5
54	30910.0
55	25644.5
56	20379.0
57	17929.0
58	15479.0
59	15513.0
60	15547.0
61	14550.5
62	13554.0
63	12413.5
64	9047.5
65	6822.0
66	5571.0
67	4320.0
68	3407.5
69	2495.0
70	2198.5
71	1902.0
72	1525.0
73	1148.0
74	890.5
75	633.0
76	570.5
77	508.0
78	429.0
79	350.0
80	228.0
81	106.0
82	65.5
83	25.0
84	19.0
85	13.0
86	6.5
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	527357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.752114597748136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1903990506647	16.19732117460143
2	11.675615823052574	5.312898984107471
3	4.38062782847317	2.990056390905184
4	2.000586663716463	1.820703081424143
5	1.1227196229061183	1.2772122760750286
6	0.7142223151822042	0.9750040777976698
7	0.511420051569484	0.8145121334626613
8	0.39043940954489365	0.7106657751554029
9	0.2995022714906379	0.6132879002916781
>10	5.936644007272595	38.97779854199611
>50	1.5725932765366923	22.37653012406577
>100	0.19855554366882378	6.488960265519629
>500	0.004171334951025709	0.63340725460829
>1k	0.0025028009706154256	0.8116420199895259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1633	0.3096574047561709	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	1498	0.28405804796371337	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1145	0.2171204705730653	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	840	0.15928488670862434	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	819	0.15530276454090872	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	566	0.1073276736631921	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	565	0.1071380487980628	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	547	0.10372480122573513	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3056752825884553	0.0
2	0.0	0.0	0.0	1.0565897485005413	0.0
3	0.0	0.0	0.0	1.7092785342756425	0.0
4	0.0	0.0	0.0	2.1057841272610394	0.0
5	0.0	0.0	0.0	4.144441052266302	0.0
6	0.0	0.0	0.0	4.504159421416611	0.0
7	0.0	0.0	0.0	5.9764068742806105	0.0
8	0.0	0.0	0.0	7.713560263730263	0.0
9	1.8962486512931468E-4	0.0	0.0	9.181067094966028	0.0
10	1.8962486512931468E-4	0.0	0.0	9.9609941652429	0.0
11	1.8962486512931468E-4	0.0	0.0	10.717407752243735	0.0
12	1.8962486512931468E-4	0.0	0.0	11.442343611633106	0.0
13	1.8962486512931468E-4	0.0	0.0	11.688097436840698	0.0
14	1.8962486512931468E-4	0.0	0.0	11.897632912808591	0.0
15	1.8962486512931468E-4	0.0	0.0	12.643806757092444	0.0
16	1.8962486512931468E-4	0.0	0.0	13.49161952908561	0.0
17	1.8962486512931468E-4	0.0	0.0	14.8227860822934	0.0
18	1.8962486512931468E-4	0.0	0.0	15.279592382389918	0.0
19	1.8962486512931468E-4	0.0	0.0	15.825522369097214	0.0
20	3.7924973025862935E-4	0.0	0.0	16.533012740894687	0.0
21	3.7924973025862935E-4	0.0	0.0	17.099991087631338	0.0
22	5.68874595387944E-4	0.0	0.0	17.793828469139502	0.0
23	5.68874595387944E-4	0.0	0.0	18.23717140381184	0.0
24	5.68874595387944E-4	0.0	0.0	18.598027522152925	0.0
25	5.68874595387944E-4	0.0	0.0	18.996429363789616	0.0
26	5.68874595387944E-4	0.0	0.0	19.337374871292123	0.0
27	5.68874595387944E-4	0.0	0.0	19.697282865307564	0.0
28	5.68874595387944E-4	0.0	0.0	20.037469873349554	0.0
29	5.68874595387944E-4	0.0	0.0	20.570884618958313	0.0
30	5.68874595387944E-4	0.0	0.0	21.06201301964324	0.0
31	5.68874595387944E-4	0.0	0.0	21.444486372609067	0.0
32	5.68874595387944E-4	0.0	0.0	21.795481997963428	0.0
33	5.68874595387944E-4	0.0	0.0	22.15538999197887	0.0
34	5.68874595387944E-4	0.0	0.0	22.77754917446815	0.0
35	5.68874595387944E-4	0.0	0.0	23.163246150141177	0.0
36	5.68874595387944E-4	0.0	0.0	23.55501112149834	0.0
37	5.68874595387944E-4	0.0	0.0	23.9092303695599	0.0
38	5.68874595387944E-4	0.0	0.0	24.248469253276244	0.0
39	5.68874595387944E-4	0.0	0.0	24.64288897274522	0.0
40	5.68874595387944E-4	0.0	0.0	25.06897604469079	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.0181611E-7	46.000004	4
CTAGCGG	140	0.0	46.000004	1
TGATCGA	20	6.3083315E-4	46.0	44
TCACGAC	25	3.414624E-5	46.0	24
GTGCATT	20	6.3083315E-4	46.0	12
TTAGGTT	20	6.3083315E-4	46.0	39
AACCGTT	25	3.414624E-5	46.0	40
GTCGCTA	20	6.3083315E-4	46.0	21
CTCACGA	25	3.414624E-5	46.0	23
ACCGGGT	20	6.3083315E-4	46.0	3
CGTATTA	25	3.414624E-5	46.0	34
CTCGTGA	30	1.8594728E-6	46.0	39
CGACGGT	20	6.3083315E-4	46.0	27
CTATCGG	25	3.414624E-5	46.0	1
CCTAGAA	25	3.414624E-5	46.0	44
TAGACAA	20	6.3083315E-4	46.0	33
TCACACG	20	6.3083315E-4	46.0	21
ATAATGG	50	1.6370905E-11	46.0	1
GACGTCA	20	6.3083315E-4	46.0	43
GAATCCG	20	6.3083315E-4	46.0	35
>>END_MODULE