FastQCFastQC Report
Thu 26 May 2016
SRR1544829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544829_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences385287
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT24380.6327750482108142Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT18190.4721155917536797Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT13520.3509072457674409Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTTG8980.2330730079135813Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA7990.2073778767516163No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC5520.1432698222364108Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT5230.13574296563341093Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC4680.1214678927656526Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC4620.1199106120891699Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4410.11446012972148034No Hit
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC3950.1025209778684461Illumina Single End Adapter 2 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG3870.10044460363313583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA206.306204E-446.0000048
CGGGTAA206.306204E-446.0000045
AGCCGTT206.306204E-446.00000427
CTGTCGG405.5952114E-946.0000041
ATCTCGG405.5952114E-946.0000041
GCAAGTT206.306204E-446.00000439
ACCGGGT206.306204E-446.0000043
TTAGCCA206.306204E-446.00000410
GTTTAGG405.5952114E-946.0000041
TAGGTAC206.306204E-446.00000441
TAGCGGC206.306204E-446.0000042
ACTTCGC206.306204E-446.00000425
CTCTTGG206.306204E-446.0000041
CTGACTA551.8189894E-1246.00000422
TAACGGA206.306204E-446.00000428
ACGGGTC351.01719706E-746.0000044
ACGGGTA206.306204E-446.0000044
CTAACGG206.306204E-446.0000041
TCTACGG405.5952114E-946.0000041
TCGTCTT206.306204E-446.00000442
Produced by FastQC (version 0.11.5)