##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544829_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	385287
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97600749571099	34.0	33.0	34.0	31.0	34.0
2	33.2968644153579	34.0	34.0	34.0	31.0	34.0
3	33.385040242728145	34.0	34.0	34.0	31.0	34.0
4	36.611230070051676	37.0	37.0	37.0	35.0	37.0
5	36.59286194447256	37.0	37.0	37.0	35.0	37.0
6	36.55075307498047	37.0	37.0	37.0	35.0	37.0
7	36.455390397288255	37.0	37.0	37.0	35.0	37.0
8	36.49323491319458	37.0	37.0	37.0	35.0	37.0
9	38.33235484197494	39.0	39.0	39.0	37.0	39.0
10	38.11527510660884	39.0	39.0	39.0	35.0	39.0
11	37.200092398653474	39.0	35.0	39.0	35.0	39.0
12	37.10094293344961	39.0	35.0	39.0	35.0	39.0
13	37.09327592158573	39.0	35.0	39.0	35.0	39.0
14	38.246239816033246	40.0	35.0	41.0	35.0	41.0
15	38.3352955069857	40.0	35.0	41.0	35.0	41.0
16	38.358130432638525	40.0	35.0	41.0	35.0	41.0
17	38.48625050936055	40.0	36.0	41.0	35.0	41.0
18	38.47256201221427	40.0	36.0	41.0	35.0	41.0
19	38.34804963572609	40.0	35.0	41.0	35.0	41.0
20	38.261558266954246	40.0	35.0	41.0	35.0	41.0
21	38.2148683968055	40.0	35.0	41.0	35.0	41.0
22	38.2018495303501	40.0	35.0	41.0	35.0	41.0
23	38.1686015879072	40.0	35.0	41.0	35.0	41.0
24	38.11116648109072	40.0	35.0	41.0	35.0	41.0
25	38.07467160843735	40.0	35.0	41.0	35.0	41.0
26	38.01920386620883	40.0	35.0	41.0	35.0	41.0
27	38.043676532039754	40.0	35.0	41.0	35.0	41.0
28	38.08759963351995	40.0	35.0	41.0	35.0	41.0
29	38.13078043121102	40.0	35.0	41.0	35.0	41.0
30	38.03850895566163	40.0	35.0	41.0	35.0	41.0
31	37.98622325694872	40.0	35.0	41.0	35.0	41.0
32	37.95801830842982	40.0	35.0	41.0	35.0	41.0
33	37.904367393657196	40.0	35.0	41.0	35.0	41.0
34	37.845094695642466	40.0	35.0	41.0	35.0	41.0
35	37.763337460127126	40.0	35.0	41.0	35.0	41.0
36	37.708116287339045	40.0	35.0	41.0	35.0	41.0
37	37.65269526353082	40.0	35.0	41.0	35.0	41.0
38	37.628861082777256	40.0	35.0	41.0	35.0	41.0
39	37.56599885280323	39.0	35.0	41.0	35.0	41.0
40	37.53111836111782	39.0	35.0	41.0	35.0	41.0
41	37.52481137437806	39.0	35.0	41.0	35.0	41.0
42	37.4356700329884	39.0	35.0	41.0	35.0	41.0
43	37.33632331223218	39.0	35.0	41.0	35.0	41.0
44	37.209547687827516	39.0	35.0	41.0	35.0	41.0
45	37.207883992971475	38.0	35.0	41.0	35.0	41.0
46	37.1490758836919	38.0	35.0	41.0	35.0	41.0
47	37.094537318933675	38.0	35.0	41.0	35.0	41.0
48	37.10175271940138	38.0	35.0	41.0	35.0	41.0
49	37.02376150765533	37.0	35.0	41.0	35.0	41.0
50	36.92901914676592	37.0	35.0	41.0	35.0	41.0
51	36.85290964917062	37.0	35.0	41.0	34.0	41.0
52	36.434416422043824	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	8.0
16	12.0
17	19.0
18	22.0
19	48.0
20	90.0
21	139.0
22	244.0
23	386.0
24	640.0
25	1051.0
26	1458.0
27	1726.0
28	1676.0
29	1682.0
30	2164.0
31	2653.0
32	3523.0
33	5404.0
34	18904.0
35	107262.0
36	11386.0
37	17393.0
38	32028.0
39	173293.0
40	2069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.551770498355772	21.519283027976545	24.379488537116487	23.549457936551192
2	24.277486652806868	25.078967107636647	27.93527941508538	22.708266824471107
3	25.447523534404226	25.173442135343265	26.443923620573756	22.935110709678757
4	25.054569710371748	27.726084710877863	24.69950971613369	22.5198358626167
5	22.84686480467807	34.880491685418924	21.545237705917927	20.72740580398508
6	86.52666713385086	2.686049620153288	7.3615253045132585	3.425757941482583
7	87.99180870364171	2.054831852618957	5.317853963409095	4.635505480330247
8	78.84667793099689	3.940698751839538	10.6453111576565	6.567312159507069
9	54.936190424281115	18.027340657743448	13.34589539745696	13.69057352051847
10	48.196798750022715	17.282181854046463	22.955355358473035	11.565664037457791
11	45.23770591792612	17.162530788736706	21.59896388925658	16.000799404080595
12	18.598084025674368	41.11324804626162	24.273333904336248	16.015334023727767
13	11.746049049150374	42.98016803058499	26.933947940106982	18.339834980157647
14	9.737935616825899	23.330140907946546	49.01255427772024	17.91936919750731
15	10.549278849273398	15.837025386270495	52.5639328604391	21.049762904017005
16	11.822615349077441	20.199228107878024	27.54829516697942	40.42986137606511
17	17.51214030060708	23.346232808270198	34.0875762743098	25.054050616812923
18	24.76880870623717	26.461832348353305	25.86461520892218	22.904743736487347
19	28.67628547031174	21.680202031213096	24.60295831419176	25.040554184283405
20	17.35719087329705	31.9603308702344	26.587193442810165	24.09528481365839
21	19.554513907814176	25.614412113567287	24.220386361335837	30.6106876172827
22	16.50224378190803	31.943200782793085	21.63114768990389	29.923407745394993
23	16.634612639409063	21.891213562876505	40.95777952539276	20.516394272321673
24	16.349370728833314	23.029326190605964	35.99602374333938	24.625279337221347
25	13.833064702416639	35.481082932982424	27.241510873712322	23.44434149088861
26	15.559310332297741	33.705523415012706	26.268729544469448	24.466436708220108
27	17.137614297912982	28.544695253148948	37.82946219311838	16.48822825581969
28	14.43884688556842	24.65045537482448	34.76135971366799	26.149338025939105
29	16.804096686366268	32.93830313506555	27.938653523217756	22.318946655350427
30	27.728420631892593	29.3908177540379	21.08298489178197	21.79777672228754
31	31.746464323997436	27.51870683412625	21.144237931723627	19.59059091015269
32	24.920643572194233	33.00604484449255	21.13826835579711	20.93504322751611
33	18.462860153599784	28.6547430876204	26.59082709772196	26.291569661057864
34	21.651392338698166	26.526978589986168	27.989524691982858	23.832104379332808
35	31.66652391593799	23.38801983975582	24.775297375722513	20.17015886858368
36	22.013979189539228	29.42949022417055	26.76134933179682	21.795181254493404
37	22.560065613425838	28.900793434504667	26.399281574514582	22.139859377554913
38	21.53044353949134	28.53016063350178	25.2154887136083	24.72390711339858
39	28.14966505488117	23.37166839265275	23.975633748348635	24.50303280411745
40	16.69197247765951	20.666152764043428	30.852066122137522	31.78980863615954
41	23.207634828063238	24.660837246001034	21.98205493567133	30.149472990264396
42	22.858025316192865	24.567659952191484	25.106998159813333	27.467316571802318
43	23.125358498989065	28.800867924430364	26.362166385058412	21.711607191522162
44	16.74881322235113	35.2583917962454	23.772408620067637	24.220386361335837
45	22.856208488736968	29.65218136090758	25.14930428485778	22.342305865497668
46	24.725983487633894	24.129285441761596	26.014892794202765	25.129838276401745
47	23.05709769600324	20.04531686768565	32.15395276767708	24.74363266863403
48	19.973162863008614	18.701902737439884	30.090815418116883	31.234118981434616
49	20.01209487992068	26.65467560545775	30.167122171264538	23.16610734335703
50	18.893967354206083	28.60906285444357	26.53943683539803	25.957532955952317
51	21.11023730362042	21.155138896459004	29.9950426565132	27.73958114340738
52	23.93436581042184	19.877390101404927	31.100971483595348	25.087272604577887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	55.5
2	68.0
3	367.5
4	667.0
5	512.0
6	357.0
7	412.5
8	468.0
9	492.0
10	516.0
11	513.5
12	511.0
13	496.5
14	497.5
15	513.0
16	468.0
17	423.0
18	454.0
19	485.0
20	463.0
21	441.0
22	534.5
23	628.0
24	637.0
25	646.0
26	939.5
27	1233.0
28	1648.5
29	2064.0
30	2397.0
31	2730.0
32	2953.5
33	3177.0
34	4346.5
35	5516.0
36	6453.0
37	7390.0
38	8255.0
39	10239.0
40	11358.0
41	17225.5
42	23093.0
43	31374.0
44	39655.0
45	44618.5
46	49582.0
47	50374.0
48	51166.0
49	48717.5
50	46269.0
51	40535.5
52	34802.0
53	29375.5
54	23949.0
55	19576.5
56	15204.0
57	13150.5
58	11097.0
59	10946.5
60	10796.0
61	10165.5
62	9535.0
63	8919.0
64	6520.0
65	4737.0
66	3889.0
67	3041.0
68	2442.0
69	1843.0
70	1585.5
71	1328.0
72	1108.0
73	888.0
74	676.5
75	465.0
76	416.0
77	367.0
78	302.0
79	237.0
80	152.0
81	67.0
82	42.5
83	18.0
84	11.5
85	5.0
86	3.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	385287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.197542799397077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62778356295819	18.502743827171507
2	11.620708871453102	6.088680360384519
3	4.175426001281833	3.2815770412288865
4	1.8955979154879694	1.9864003008577582
5	1.066770320905158	1.3973380569519716
6	0.7218444862506198	1.1346331093835642
7	0.509890146602674	0.935050825703008
8	0.4389054398097777	0.9198595235443876
9	0.3719198715432934	0.8769048077431523
>10	8.042267329819461	51.19783051944099
>50	0.43890543980491553	7.7331584374564875
>100	0.08298212187655578	3.7506800617909195
>500	0.003999138403689435	0.7260394644751698
>1k	0.0029993538027670765	1.4691036638676864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	2438	0.6327750482108142	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	1819	0.4721155917536797	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1352	0.3509072457674409	Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTTG	898	0.2330730079135813	Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	799	0.2073778767516163	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	552	0.1432698222364108	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	523	0.13574296563341093	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	468	0.1214678927656526	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	462	0.1199106120891699	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	441	0.11446012972148034	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	395	0.1025209778684461	Illumina Single End Adapter 2 (95% over 21bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	387	0.10044460363313583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5006657374891964	0.0
2	0.0	0.0	0.0	1.7877582166021693	0.0
3	0.0	0.0	0.0	2.7260198241830116	0.0
4	0.0	0.0	0.0	3.3201224022611715	0.0
5	0.0	0.0	0.0	6.292452120107868	0.0
6	0.0	0.0	0.0	6.805316556229512	0.0
7	0.0	0.0	0.0	8.924256463363674	0.0
8	0.0	0.0	0.0	11.278864846205556	0.0
9	0.0	0.0	0.0	13.36847596726596	0.0
10	0.0	0.0	0.0	14.48063391705404	0.0
11	0.0	0.0	0.0	15.509477350650295	0.0
12	0.0	0.0	0.0	16.51911432256993	0.0
13	0.0	0.0	0.0	16.870021568337368	0.0
14	0.0	0.0	0.0	17.160973508060223	0.0
15	0.0	0.0	0.0	18.097158741405757	0.0
16	0.0	0.0	0.0	19.115360757046044	0.0
17	0.0	0.0	0.0	20.63967899254322	0.0
18	0.0	0.0	0.0	21.197185474724037	0.0
19	0.0	0.0	0.0	21.852541092743852	0.0
20	0.0	0.0	0.0	22.714236400397624	0.0
21	0.0	0.0	0.0	23.413195877358955	0.0
22	0.0	0.0	0.0	24.226615484041766	0.0
23	0.0	0.0	0.0	24.776076016060756	0.0
24	0.0	0.0	0.0	25.217565087843607	0.0
25	0.0	0.0	0.0	25.688123398920805	0.0
26	0.0	0.0	0.0	26.057458466026624	0.0
27	0.0	0.0	0.0	26.48077926325051	0.0
28	0.0	0.0	0.0	26.854267078826954	0.0
29	0.0	0.0	0.0	27.44188098741977	0.0
30	0.0	0.0	0.0	27.993417893674067	0.0
31	0.0	0.0	0.0	28.39260084041247	0.0
32	0.0	0.0	0.0	28.788150132239085	0.0
33	2.595467794137876E-4	0.0	0.0	29.190447640330454	0.0
34	2.595467794137876E-4	0.0	0.0	29.820886767526545	0.0
35	2.595467794137876E-4	0.0	0.0	30.24135255017688	0.0
36	2.595467794137876E-4	0.0	0.0	30.645466885724147	0.0
37	2.595467794137876E-4	0.0	0.0	31.01220648503583	0.0
38	2.595467794137876E-4	0.0	0.0	31.381282005362237	0.0
39	2.595467794137876E-4	0.0	0.0	31.797075945983124	0.0
40	2.595467794137876E-4	0.0	0.0	32.24427504691308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAA	20	6.306204E-4	46.000004	8
CGGGTAA	20	6.306204E-4	46.000004	5
AGCCGTT	20	6.306204E-4	46.000004	27
CTGTCGG	40	5.5952114E-9	46.000004	1
ATCTCGG	40	5.5952114E-9	46.000004	1
GCAAGTT	20	6.306204E-4	46.000004	39
ACCGGGT	20	6.306204E-4	46.000004	3
TTAGCCA	20	6.306204E-4	46.000004	10
GTTTAGG	40	5.5952114E-9	46.000004	1
TAGGTAC	20	6.306204E-4	46.000004	41
TAGCGGC	20	6.306204E-4	46.000004	2
ACTTCGC	20	6.306204E-4	46.000004	25
CTCTTGG	20	6.306204E-4	46.000004	1
CTGACTA	55	1.8189894E-12	46.000004	22
TAACGGA	20	6.306204E-4	46.000004	28
ACGGGTC	35	1.01719706E-7	46.000004	4
ACGGGTA	20	6.306204E-4	46.000004	4
CTAACGG	20	6.306204E-4	46.000004	1
TCTACGG	40	5.5952114E-9	46.000004	1
TCGTCTT	20	6.306204E-4	46.000004	42
>>END_MODULE