FastQCFastQC Report
Thu 26 May 2016
SRR1544828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544828_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences653237
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT43190.6611689172536154No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT39050.597792225486309Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT29470.45113794840157556No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.21783824247554867No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT12090.1850783100161197No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC11960.1830882206611077No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC11830.18109813130609564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10590.16211574053521155No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC9680.1481851150501273No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT9250.14160251179893363No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC9110.1394593386473822No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT8320.12736571872077057No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG7460.11420051221838323No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7400.11328200943914689No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG7290.11159808767721363No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT7180.10991416591528036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG351.01867045E-746.0000041
TATGGGT351.01867045E-746.0000043
AATGCGG700.046.0000041
CCGTAGG351.01867045E-746.0000041
TCACGAC301.8601604E-646.024
CGTATTT301.8601604E-646.04
AATTTCG453.092282E-1046.036
CATGCGG1050.046.01
GACGTAT301.8601604E-646.02
ATTACGG405.6043064E-946.01
CGCGCAT253.415525E-546.042
CACGACG301.8601604E-646.025
TACGGGA850.046.03
GCGCATT253.415525E-546.043
ACGGGTA600.046.04
TTATACG253.415525E-546.09
GTTACGG405.6043064E-946.01
CTAACGG405.6043064E-946.01
CACTAGG551.8189894E-1246.01
CGAGGGT253.415525E-546.03
Produced by FastQC (version 0.11.5)