##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544828_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	653237
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.846971313627364	34.0	31.0	34.0	31.0	34.0
2	33.1550738858944	34.0	33.0	34.0	31.0	34.0
3	33.198107271939584	34.0	33.0	34.0	31.0	34.0
4	36.50992518794863	37.0	37.0	37.0	35.0	37.0
5	36.51025584894916	37.0	37.0	37.0	35.0	37.0
6	36.62903050500814	37.0	37.0	37.0	35.0	37.0
7	36.59128922580931	37.0	37.0	37.0	35.0	37.0
8	36.48179297865859	37.0	37.0	37.0	35.0	37.0
9	38.28521501384643	39.0	39.0	39.0	37.0	39.0
10	38.06515399464513	39.0	39.0	39.0	35.0	39.0
11	37.32943173763886	39.0	35.0	39.0	35.0	39.0
12	37.19520786483313	39.0	35.0	39.0	35.0	39.0
13	37.17473749955988	39.0	35.0	39.0	35.0	39.0
14	38.45374190378071	40.0	35.0	41.0	35.0	41.0
15	38.53244534525754	41.0	35.0	41.0	35.0	41.0
16	38.531050751871064	41.0	35.0	41.0	35.0	41.0
17	38.6001680860086	41.0	36.0	41.0	35.0	41.0
18	38.570942858411264	40.0	36.0	41.0	35.0	41.0
19	38.49271244586574	40.0	35.0	41.0	35.0	41.0
20	38.39033153357816	40.0	35.0	41.0	35.0	41.0
21	38.33494887766614	40.0	35.0	41.0	35.0	41.0
22	38.31926697354865	40.0	35.0	41.0	35.0	41.0
23	38.28606769059316	40.0	35.0	41.0	35.0	41.0
24	38.223735030318245	40.0	35.0	41.0	35.0	41.0
25	38.20203080964489	40.0	35.0	41.0	35.0	41.0
26	38.142173514360024	40.0	35.0	41.0	35.0	41.0
27	38.128743472889624	40.0	35.0	41.0	35.0	41.0
28	38.18060979399514	40.0	35.0	41.0	35.0	41.0
29	38.1735602851645	40.0	35.0	41.0	35.0	41.0
30	38.09839001771179	40.0	35.0	41.0	35.0	41.0
31	38.03348095714113	40.0	35.0	41.0	35.0	41.0
32	37.95772590958565	40.0	35.0	41.0	35.0	41.0
33	37.85111376116172	40.0	35.0	41.0	35.0	41.0
34	37.77539086120351	40.0	35.0	41.0	35.0	41.0
35	37.70656591711737	40.0	35.0	41.0	35.0	41.0
36	37.61295823720947	40.0	35.0	41.0	34.0	41.0
37	37.545754450528676	40.0	35.0	41.0	34.0	41.0
38	37.50095753914735	40.0	35.0	41.0	34.0	41.0
39	37.40288899740829	40.0	35.0	41.0	34.0	41.0
40	37.31627265448834	39.0	35.0	41.0	34.0	41.0
41	37.304825048183126	39.0	35.0	41.0	34.0	41.0
42	37.21542564184209	39.0	35.0	41.0	34.0	41.0
43	37.20447249619969	39.0	35.0	41.0	34.0	41.0
44	37.16872436803182	39.0	35.0	41.0	34.0	41.0
45	37.14881275861594	39.0	35.0	41.0	34.0	41.0
46	37.123612104029625	39.0	35.0	41.0	34.0	41.0
47	37.00528445265654	38.0	35.0	41.0	34.0	41.0
48	37.023444783440006	38.0	35.0	41.0	34.0	41.0
49	36.957121228589315	38.0	35.0	41.0	34.0	41.0
50	36.86022377789378	38.0	35.0	41.0	34.0	41.0
51	36.778319354231314	37.0	35.0	41.0	34.0	41.0
52	36.52801203850976	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	4.0
16	16.0
17	14.0
18	46.0
19	82.0
20	165.0
21	311.0
22	516.0
23	850.0
24	1376.0
25	2267.0
26	3156.0
27	3816.0
28	3834.0
29	4379.0
30	5199.0
31	6450.0
32	8352.0
33	11763.0
34	34349.0
35	139134.0
36	25606.0
37	36825.0
38	62365.0
39	299525.0
40	2834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4709102515626	22.270630720550123	24.41166069895	20.846798328937275
2	27.987238934720477	23.81570547902216	26.228153028686375	21.96890255757099
3	26.346792971004398	24.76926444766601	26.69123151321802	22.192711068111574
4	26.028684841795553	26.687863669694156	24.196577964812157	23.086873523698138
5	24.44886006150907	35.116657507152844	20.550275015040484	19.88420741629761
6	84.73264680353378	2.744792471951221	9.074348207465285	3.4482125170497078
7	85.44142478151116	2.931554703729274	6.494120816793905	5.132899697965669
8	73.97085590681483	5.4681532123869285	12.854905646802003	7.706085233996237
9	49.97129678814887	20.109393681007045	15.327821296099273	14.591488234744817
10	42.485652221169346	19.374438373821445	25.272144719297895	12.867764685711311
11	40.22460454628259	17.07312966044483	24.333587962714912	18.36867783055767
12	20.639829036016025	33.366297377521484	27.846861093293857	18.147012493168635
13	13.507348787652873	37.089754560749014	29.490368732940723	19.912527918657393
14	12.735041034111664	24.558008808441652	44.206007926678986	18.5009422307677
15	14.407787678897552	18.275449798465182	46.97912090098999	20.337641621647272
16	14.753757059076566	21.94593998809008	27.02510727347042	36.27519567936292
17	18.93799646988765	23.2561842026707	32.1572415524534	25.64857777498825
18	23.430240479335986	28.29907062827121	27.50196329969062	20.76872559270219
19	27.487114171426295	21.90843445793793	25.878662721186952	24.72578864944882
20	19.5763559014569	29.958958234147794	27.855127618306984	22.609558246088326
21	19.741380234126357	24.1563169263223	26.502173024491878	29.600129815059468
22	18.505228577070802	28.52532847955642	23.60353133701857	29.3659116063542
23	18.342959752739052	22.697734512895014	36.92488331187609	22.034422422489847
24	19.53150234907086	21.225221473982643	34.19815472791651	25.045121449029985
25	15.559590164059905	31.471579227753484	28.17109257436428	24.797738033822334
26	16.63837167827297	30.322072999539216	27.552940203938235	25.486615118249578
27	18.7108201158232	27.309230799847526	34.3663938203133	19.613555264015968
28	15.727523088863613	26.197689353175036	30.49842553315259	27.57636202480876
29	19.142822589657353	30.09688673482978	26.16845034803601	24.59184032747686
30	26.40358705952051	24.556937221865876	21.616809825530396	27.422665893083213
31	27.211869505248476	29.63288974751889	21.220935127679542	21.934305619553086
32	25.595916948978704	30.01850783100161	20.846492161344198	23.539083058675487
33	16.59596746663156	29.18925290514775	24.02696111824652	30.187818509974175
34	21.725805488666442	26.042156215891016	25.905452385581345	26.3265859098612
35	26.875544404251446	27.97024663330461	22.48326411394333	22.67094484850062
36	22.61598776554298	30.703098569125753	23.597714152750076	23.083199512581192
37	21.684932114990424	29.70453296429933	24.643123399317552	23.967411521392695
38	20.6510041531634	30.260992564720002	23.604909091187427	25.483094190929172
39	26.350007730731722	26.08149875160164	24.9266345905085	22.641858927158136
40	16.29561705782128	23.324612659723805	30.12428873441033	30.255481548044582
41	21.080404202456382	28.18686020540784	22.38574973554774	28.346985856588034
42	22.246749648289978	23.907402673149257	26.118238862771094	27.727608815789672
43	22.829386577918886	28.47726016744306	25.48340035852225	23.209952896115805
44	19.68933174330297	29.717238919412097	23.822441166069893	26.770988171215045
45	21.91394547461335	27.345358575830826	24.217244277344978	26.52345167221085
46	24.63363220393211	24.344150744676128	25.924740943945306	25.097476107446454
47	21.08392512977679	22.70921579763547	31.440043965666366	24.766815106921378
48	19.757300948966456	21.501537726736238	27.688266280079056	31.052895044218253
49	21.13184035809362	24.02665495065344	30.348403412543995	24.493101278708952
50	19.227631012940176	25.54356229056223	27.88880605354565	27.340000642951946
51	19.97881320255895	21.705292259930165	28.79659296702422	29.519301570486668
52	20.750202453320924	19.542830550014774	34.38797863562535	25.318988361038947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	246.0
2	338.0
3	1439.0
4	2540.0
5	1731.5
6	923.0
7	1045.5
8	1168.0
9	1152.5
10	1137.0
11	1087.0
12	1037.0
13	973.5
14	906.5
15	903.0
16	871.0
17	839.0
18	789.5
19	740.0
20	863.0
21	986.0
22	1068.0
23	1150.0
24	1297.0
25	1444.0
26	2097.5
27	2751.0
28	3585.5
29	4420.0
30	4705.0
31	4990.0
32	5695.0
33	6400.0
34	7461.5
35	8523.0
36	9087.0
37	9651.0
38	10843.0
39	14353.5
40	16672.0
41	24887.5
42	33103.0
43	43990.5
44	54878.0
45	62841.5
46	70805.0
47	77030.5
48	83256.0
49	84245.5
50	85235.0
51	73932.0
52	62629.0
53	53094.5
54	43560.0
55	37185.0
56	30810.0
57	27069.0
58	23328.0
59	21798.0
60	20268.0
61	20091.0
62	19914.0
63	18291.5
64	13640.0
65	10611.0
66	8506.0
67	6401.0
68	5445.5
69	4490.0
70	3708.5
71	2927.0
72	2415.0
73	1903.0
74	1493.0
75	1083.0
76	942.5
77	802.0
78	640.0
79	478.0
80	359.0
81	240.0
82	175.5
83	111.0
84	66.5
85	22.0
86	11.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	653237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.187350306015144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.48442845989842	18.619101470464923
2	12.682334172566598	6.895981236950297
3	5.144870066449101	4.1962615432644945
4	2.543742425214456	2.7663046561031184
5	1.4186598585491295	1.928480126972854
6	0.9048740523539119	1.4760676706501579
7	0.66340778714941	1.262540993347854
8	0.4646971344006255	1.0107107023320965
9	0.3929637344297655	0.9615278434951746
>10	6.7591658748029335	43.87078389521831
>50	0.41001316752134537	7.4319588473506935
>100	0.11843571551510455	5.45046092731115
>500	0.007895714367673638	1.4862384383612393
>1k	0.004511836781527792	2.6435816481776673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	4319	0.6611689172536154	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	3905	0.597792225486309	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2947	0.45113794840157556	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.21783824247554867	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	1209	0.1850783100161197	No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1196	0.1830882206611077	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1183	0.18109813130609564	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1059	0.16211574053521155	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	968	0.1481851150501273	No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	925	0.14160251179893363	No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	911	0.1394593386473822	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	832	0.12736571872077057	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	746	0.11420051221838323	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	740	0.11328200943914689	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG	729	0.11159808767721363	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	718	0.10991416591528036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6118759347679327	0.0
2	0.0	0.0	0.0	2.0865321468318543	0.0
3	0.0	0.0	0.0	3.2686452237090062	0.0
4	0.0	0.0	0.0	3.9869143970718133	0.0
5	0.0	0.0	0.0	7.477990377152549	0.0
6	0.0	0.0	0.0	8.046543597499836	0.0
7	0.0	0.0	0.0	10.25783903851129	0.0
8	0.0	0.0	0.0	12.931906796461314	0.0
9	0.0	0.0	0.0	15.07324294245427	0.0
10	0.0	0.0	0.0	16.376445302394078	0.0
11	0.0	0.0	0.0	17.705518823949042	0.0
12	0.0	0.0	0.0	18.927892939316052	0.0
13	1.5308379653938768E-4	0.0	0.0	19.38806283171345	0.0
14	1.5308379653938768E-4	0.0	0.0	19.740002479957504	0.0
15	1.5308379653938768E-4	0.0	0.0	20.957018662445638	0.0
16	1.5308379653938768E-4	0.0	0.0	22.24552497791766	0.0
17	1.5308379653938768E-4	0.0	0.0	24.25413747231097	0.0
18	1.5308379653938768E-4	0.0	0.0	25.021699628159457	0.0
19	1.5308379653938768E-4	0.0	0.0	25.942345580547336	0.0
20	3.0616759307877537E-4	0.0	0.0	27.024341854487727	0.0
21	3.0616759307877537E-4	0.0	0.0	27.904114433199588	0.0
22	3.0616759307877537E-4	0.0	0.0	28.905282462567186	0.0
23	3.0616759307877537E-4	0.0	0.0	29.615897446103023	0.0
24	4.592513896181631E-4	0.0	0.0	30.209862576675846	0.0
25	4.592513896181631E-4	0.0	0.0	30.794795763252846	0.0
26	4.592513896181631E-4	0.0	0.0	31.31130049277674	0.0
27	4.592513896181631E-4	0.0	0.0	31.86362683069085	0.0
28	4.592513896181631E-4	0.0	0.0	32.37691680048742	0.0
29	4.592513896181631E-4	0.0	0.0	33.115699202586505	0.0
30	4.592513896181631E-4	0.0	0.0	33.80518862219991	0.0
31	4.592513896181631E-4	0.0	0.0	34.38828480321843	0.0
32	4.592513896181631E-4	0.0	0.0	34.923618839716674	0.0
33	4.592513896181631E-4	0.0	0.0	35.418538753928516	0.0
34	4.592513896181631E-4	0.0	0.0	36.1969698593313	0.0
35	4.592513896181631E-4	0.0	0.0	36.74699994029732	0.0
36	4.592513896181631E-4	0.0	0.0	37.308817473596875	0.0
37	6.123351861575507E-4	0.0	0.0	37.81965810264881	0.0
38	6.123351861575507E-4	0.0	0.0	38.325600050211484	0.0
39	6.123351861575507E-4	0.0	0.0	38.89935811964111	0.0
40	6.123351861575507E-4	0.0	0.0	39.50878471366441	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	35	1.01867045E-7	46.000004	1
TATGGGT	35	1.01867045E-7	46.000004	3
AATGCGG	70	0.0	46.000004	1
CCGTAGG	35	1.01867045E-7	46.000004	1
TCACGAC	30	1.8601604E-6	46.0	24
CGTATTT	30	1.8601604E-6	46.0	4
AATTTCG	45	3.092282E-10	46.0	36
CATGCGG	105	0.0	46.0	1
GACGTAT	30	1.8601604E-6	46.0	2
ATTACGG	40	5.6043064E-9	46.0	1
CGCGCAT	25	3.415525E-5	46.0	42
CACGACG	30	1.8601604E-6	46.0	25
TACGGGA	85	0.0	46.0	3
GCGCATT	25	3.415525E-5	46.0	43
ACGGGTA	60	0.0	46.0	4
TTATACG	25	3.415525E-5	46.0	9
GTTACGG	40	5.6043064E-9	46.0	1
CTAACGG	40	5.6043064E-9	46.0	1
CACTAGG	55	1.8189894E-12	46.0	1
CGAGGGT	25	3.415525E-5	46.0	3
>>END_MODULE