FastQCFastQC Report
Thu 26 May 2016
SRR1544827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544827_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences521612
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT58851.1282332461676494No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT49940.9574166238506783Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT31650.6067728503178608No Hit
TGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTTG24660.47276519711969817Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18440.35351947424522445No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT13630.26130533806737577No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC12290.2356157450365406No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC12240.23465717813240494No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC10330.19803992239442345No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC9720.18634540616396864No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT9520.18251113854742604No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8380.16065581313313343No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT7190.13784192081470517No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG6540.12538055106094184No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.12480541091846047No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC6340.12154628344439929No Hit
GTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTTGA6280.12039600315943652Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC5950.11406946159214129No Hit
TGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC5450.10448379255078488No Hit
AGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5410.10371693902747636No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT5410.10371693902747636No Hit
CTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTTGAAA5370.10295008550416786Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATC253.4145713E-546.027
CTATCTG253.4145713E-546.029
CATGCGG1000.046.01
CCTAGCG253.4145713E-546.045
ATAATAG206.308269E-446.015
ATTACGG453.092282E-1046.01
TAAACGG206.308269E-446.01
GATCACT206.308269E-446.08
GTCTAGA206.308269E-446.033
ATAGAGG750.046.01
CACGAGG453.092282E-1046.01
GGATAGT253.4145713E-546.07
TAGCCGG253.4145713E-546.01
TCGGCGT2400.046.04
ACTAGTG405.6006684E-946.011
ACTAGTA206.308269E-446.041
AATGCGG750.046.01
CTAACGG301.8594346E-646.01
CCATAGG405.6006684E-946.01
CGCAGGT600.046.038
Produced by FastQC (version 0.11.5)