FastQCFastQC Report
Thu 26 May 2016
SRR1544826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544826_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences445449
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT29060.6523754683476671No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT25980.5832317504360769Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT19440.4364135961692584No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.17981856508825927No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC7870.17667566881955063No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT7600.17061436887275536No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC7020.15759379861667666No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC6770.1519814838511255No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT6030.13536903214509405No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC5830.13087918033265314No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT5780.1297567173795429No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4820.10820542867982642No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG4600.1032665916861414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAT351.0176882E-746.00000422
ATAAGCG351.0176882E-746.00000432
GCTACGA700.046.00000410
GACGAGG351.0176882E-746.0000041
AGTACGG351.0176882E-746.0000041
AAACTAG301.858818E-646.09
TCGTTGA253.413762E-546.023
AATCCCC501.6370905E-1146.036
GTACCCT206.307271E-446.017
CTCAATC253.413762E-546.026
GTACACG206.307271E-446.024
TTAGCGG600.046.01
CCCTACA850.046.026
AGCCCGA253.413762E-546.014
CTTTTCG206.307271E-446.031
GACGTTG206.307271E-446.038
GGCGTAC206.307271E-446.07
ACTTAGC206.307271E-446.015
GCGTTGG453.092282E-1046.01
ACGGGTG405.5988494E-946.04
Produced by FastQC (version 0.11.5)