##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544826_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	445449
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89656054901908	34.0	33.0	34.0	31.0	34.0
2	33.18816295468168	34.0	33.0	34.0	31.0	34.0
3	33.22051458191623	34.0	33.0	34.0	31.0	34.0
4	36.525094904242685	37.0	37.0	37.0	35.0	37.0
5	36.51693235364767	37.0	37.0	37.0	35.0	37.0
6	36.65132259809765	37.0	37.0	37.0	35.0	37.0
7	36.618263819202646	37.0	37.0	37.0	35.0	37.0
8	36.52514429261262	37.0	37.0	37.0	35.0	37.0
9	38.31251164555314	39.0	39.0	39.0	37.0	39.0
10	38.083984923077615	39.0	39.0	39.0	35.0	39.0
11	37.17704159174227	39.0	35.0	39.0	35.0	39.0
12	37.00641150838817	39.0	35.0	39.0	35.0	39.0
13	36.97722522668139	39.0	35.0	39.0	35.0	39.0
14	38.198217977815645	40.0	35.0	41.0	35.0	41.0
15	38.28465211505694	40.0	35.0	41.0	35.0	41.0
16	38.304569097696934	40.0	35.0	41.0	35.0	41.0
17	38.39772005324964	40.0	36.0	41.0	35.0	41.0
18	38.37701734654248	40.0	36.0	41.0	35.0	41.0
19	38.27579363743099	40.0	35.0	41.0	35.0	41.0
20	38.16998803454492	40.0	35.0	41.0	35.0	41.0
21	38.12233948218539	40.0	35.0	41.0	35.0	41.0
22	38.12108456860381	40.0	35.0	41.0	35.0	41.0
23	38.08046487925666	40.0	35.0	41.0	35.0	41.0
24	38.033123881746285	40.0	35.0	41.0	35.0	41.0
25	38.01873839653922	40.0	35.0	41.0	35.0	41.0
26	37.9686024662756	40.0	35.0	41.0	35.0	41.0
27	37.97625990854172	40.0	35.0	41.0	35.0	41.0
28	37.99678526610229	40.0	35.0	41.0	35.0	41.0
29	38.00933889176988	40.0	35.0	41.0	35.0	41.0
30	37.91958450911328	40.0	35.0	41.0	35.0	41.0
31	37.85577249022896	40.0	35.0	41.0	35.0	41.0
32	37.782141165430836	40.0	35.0	41.0	35.0	41.0
33	37.69651520151577	40.0	35.0	41.0	35.0	41.0
34	37.622722242052404	40.0	35.0	41.0	35.0	41.0
35	37.568734018933704	40.0	35.0	41.0	35.0	41.0
36	37.493221446226165	40.0	35.0	41.0	34.0	41.0
37	37.418923378433895	39.0	35.0	41.0	34.0	41.0
38	37.395291043419114	39.0	35.0	41.0	35.0	41.0
39	37.26770516939089	39.0	35.0	41.0	34.0	41.0
40	37.19494936569618	39.0	35.0	41.0	34.0	41.0
41	37.215607173885225	39.0	35.0	41.0	34.0	41.0
42	37.127924857840064	39.0	35.0	41.0	34.0	41.0
43	37.131180000404086	39.0	35.0	41.0	34.0	41.0
44	37.102842300689865	39.0	35.0	41.0	34.0	41.0
45	37.09560690449412	39.0	35.0	41.0	34.0	41.0
46	37.080063037519444	38.0	35.0	41.0	34.0	41.0
47	36.995590965520186	38.0	35.0	41.0	34.0	41.0
48	37.03407573033052	38.0	35.0	41.0	34.0	41.0
49	36.972189857873744	38.0	35.0	41.0	34.0	41.0
50	36.88449182734724	38.0	35.0	41.0	34.0	41.0
51	36.81786018152471	38.0	35.0	41.0	34.0	41.0
52	36.57208344838578	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	2.0
16	1.0
17	12.0
18	34.0
19	63.0
20	119.0
21	193.0
22	322.0
23	521.0
24	892.0
25	1627.0
26	2259.0
27	2494.0
28	2494.0
29	2810.0
30	3316.0
31	4179.0
32	5477.0
33	8355.0
34	25073.0
35	111694.0
36	14659.0
37	20377.0
38	36499.0
39	200180.0
40	1794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.19100054102714	22.524239587472415	24.232179216924944	22.052580654575497
2	25.729544796373993	24.067401655408364	27.769508967356533	22.43354458086111
3	25.62695168245972	25.14317014966921	27.28662540492851	21.94325276294256
4	25.458806732083804	27.056520499540916	24.5300808846804	22.954591883694878
5	23.44308776088845	35.10502885852252	21.832577915765892	19.619305464823135
6	85.26722475524696	2.7527281462075344	8.745782345453689	3.234264753091824
7	86.25117577994338	2.451459089592748	5.960951758787202	5.3364133716766675
8	75.56128759970278	4.7307323621783866	12.412644320674197	7.295335717444646
9	51.38859891929267	18.09073541527762	15.15886218175369	15.36180348367602
10	46.44123120716401	18.843683564223962	24.45173297055331	10.263352258058722
11	43.87977074816646	16.24046748337071	23.369903176345666	16.509858592117165
12	19.712469889931285	37.738102453928505	26.76872099836345	15.780706657776761
13	11.388060137075176	41.45839366571706	29.261935709811898	17.891610487395866
14	11.481224562183325	23.58743649665843	48.64956482111308	16.281774120045167
15	12.21441736315493	17.01810981728548	51.196433261720195	19.57103955783939
16	12.433522131602047	20.80148344703883	28.277535699934226	38.4874587214249
17	18.20096127727304	23.2154522739977	34.15318027428505	24.430406174444215
18	23.889154538454456	28.01532835408767	28.060900349983946	20.03461675747392
19	28.265413100040632	22.288297874728645	25.09692467600107	24.349364349229656
20	17.925284375989172	31.516065812247867	28.21243284865383	22.346216963109132
21	18.02383662327225	24.653776302113148	26.569371577891072	30.753015496723528
22	16.34799943427867	30.773219829879515	23.677009040316623	29.201771695525192
23	17.723914522201195	21.72078060563611	39.0902213272451	21.465083544917597
24	17.037416180078978	22.460483691735757	35.50148277356106	25.00061735462421
25	13.440820385723168	32.77838764931563	30.035088191914227	23.74570377304697
26	15.344293061607504	30.398766188721943	30.11006871718199	24.146872032488567
27	16.98039506206098	26.25261253252336	40.015355293198546	16.75163711221711
28	13.959398270060097	24.636939357816495	35.3409705712663	26.062691800857113
29	16.646574579805993	26.116570022606407	32.70228466109476	24.534570736492842
30	26.099957570900372	24.16595390269144	25.17145621608759	24.5626323103206
31	27.26889049026937	24.36418086021071	27.10231698802781	21.264611661492115
32	22.064703254469087	27.193685472410984	26.615392558968594	24.12621871415134
33	15.05133023084573	24.80328836746743	33.1324124647266	27.012968936960235
34	18.462495145347727	22.582383168443528	33.204923571497524	25.750198114711225
35	23.494272071550277	23.915195678966615	28.547824779043168	24.04270747043994
36	20.264497170270896	28.07616584614625	27.825183129830798	23.834153853752056
37	20.108699312379194	28.59743764157064	27.12229682859317	24.171566217456995
38	18.95211348549441	30.04743528439844	25.485296857777207	25.51515437232994
39	24.670388753819182	25.775341284860893	25.756259414658018	23.798010546661907
40	14.270320508071633	21.543206966454072	33.351517233173716	30.834955292300574
41	21.08681352971945	24.56173433995811	26.621004873734144	27.7304472565883
42	20.75209507710198	22.425462847598716	30.19739633493397	26.625045740365337
43	20.936852479183923	26.67420961771157	29.68600221349694	22.702935689607564
44	15.910912360337548	30.305601763613794	26.35206275016893	27.43142312587973
45	20.437805450231114	27.322095234246795	27.134643921077384	25.10545539444471
46	23.874338027473403	22.701813226654455	28.625948200579636	24.797900545292503
47	20.660277607537562	20.165495937806575	33.84787035103906	25.3263561036168
48	18.403004608832884	19.089951936136348	29.700145246706132	32.80689820832464
49	18.876459482454784	23.918563067825946	32.604405891583546	24.600571558135723
50	18.36080000179594	25.523236105592336	28.50988553122804	27.606078361383684
51	19.25248457174671	20.21488430774342	31.73651753623872	28.79611358427115
52	20.27392585907702	18.838071249458412	35.92420232170237	24.963800569762196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	143.0
2	179.0
3	769.5
4	1360.0
5	957.0
6	554.0
7	648.5
8	743.0
9	772.0
10	801.0
11	774.5
12	748.0
13	738.0
14	634.5
15	541.0
16	554.0
17	567.0
18	601.0
19	635.0
20	686.0
21	737.0
22	748.5
23	760.0
24	861.5
25	963.0
26	1276.0
27	1589.0
28	2079.0
29	2569.0
30	2950.0
31	3331.0
32	3624.0
33	3917.0
34	4656.5
35	5396.0
36	6097.5
37	6799.0
38	7907.0
39	11401.5
40	13788.0
41	21549.5
42	29311.0
43	41730.0
44	54149.0
45	64110.0
46	74071.0
47	70862.0
48	67653.0
49	56475.0
50	45297.0
51	38040.0
52	30783.0
53	26521.5
54	22260.0
55	18531.0
56	14802.0
57	13238.0
58	11674.0
59	11636.0
60	11598.0
61	10923.0
62	10248.0
63	9152.5
64	6116.5
65	4176.0
66	3295.0
67	2414.0
68	1757.5
69	1101.0
70	937.5
71	774.0
72	632.5
73	491.0
74	417.5
75	344.0
76	289.5
77	235.0
78	163.0
79	91.0
80	79.0
81	67.0
82	41.5
83	16.0
84	12.5
85	9.0
86	4.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	445449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.12420730314859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.86065083293819	19.36651219055285
2	12.609660784051325	7.092734278260853
3	4.779115941255274	4.0322654237293625
4	2.2876104626340306	2.573489235198845
5	1.3596871751801547	1.9120061991099588
6	0.7996737167145468	1.349411363025554
7	0.6356972849003169	1.2514937555809655
8	0.48711103752547824	1.0959689439214673
9	0.41603035551628154	1.0530471566648258
>10	7.290444424321503	46.10097527802316
>50	0.37210804880930304	6.899200814832376
>100	0.09342626976542587	4.367105857401465
>500	0.006388121009601768	1.233147087472562
>1k	0.002395545378600663	1.672642416225759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	2906	0.6523754683476671	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	2598	0.5832317504360769	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1944	0.4364135961692584	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.17981856508825927	No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	787	0.17667566881955063	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	760	0.17061436887275536	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	702	0.15759379861667666	No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	677	0.1519814838511255	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	603	0.13536903214509405	No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	583	0.13087918033265314	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	578	0.1297567173795429	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	482	0.10820542867982642	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	460	0.1032665916861414	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5933339170140689	0.0
2	0.0	0.0	0.0	2.0139230304703792	0.0
3	0.0	0.0	0.0	3.161529153730281	0.0
4	0.0	0.0	0.0	3.9306407692014127	0.0
5	0.0	0.0	0.0	7.5315019227790385	0.0
6	2.2449259062204651E-4	0.0	0.0	8.131121632330524	0.0
7	2.2449259062204651E-4	0.0	0.0	10.775868842448855	0.0
8	2.2449259062204651E-4	0.0	0.0	13.84468255625223	0.0
9	2.2449259062204651E-4	0.0	0.0	16.59988012095661	0.0
10	2.2449259062204651E-4	0.0	0.0	17.993979108719518	0.0
11	2.2449259062204651E-4	0.0	0.0	19.360690000426537	0.0
12	2.2449259062204651E-4	0.0	0.0	20.66746137043747	0.0
13	2.2449259062204651E-4	0.0	0.0	21.09332381484749	0.0
14	2.2449259062204651E-4	0.0	0.0	21.42983820818994	0.0
15	2.2449259062204651E-4	0.0	0.0	22.684302804585936	0.0
16	2.2449259062204651E-4	0.0	0.0	23.964359556312843	0.0
17	2.2449259062204651E-4	0.0	0.0	26.276633239719924	0.0
18	2.2449259062204651E-4	0.0	0.0	27.0421529737411	0.0
19	2.2449259062204651E-4	0.0	0.0	28.053716587084043	0.0
20	2.2449259062204651E-4	0.0	0.0	29.087280474307946	0.0
21	4.4898518124409303E-4	0.0	0.0	29.897698726453534	0.0
22	4.4898518124409303E-4	0.0	0.0	30.910160310158965	0.0
23	4.4898518124409303E-4	0.0	0.0	31.543229415713135	0.0
24	4.4898518124409303E-4	0.0	0.0	32.053501074197044	0.0
25	4.4898518124409303E-4	0.0	0.0	32.60463038417417	0.0
26	4.4898518124409303E-4	0.0	0.0	33.05877889500257	0.0
27	4.4898518124409303E-4	0.0	0.0	33.56725461276151	0.0
28	4.4898518124409303E-4	0.0	0.0	34.02791340871794	0.0
29	4.4898518124409303E-4	0.0	0.0	34.77637170585185	0.0
30	4.4898518124409303E-4	0.0	0.0	35.46511497388029	0.0
31	4.4898518124409303E-4	0.0	0.0	36.02140761344172	0.0
32	4.4898518124409303E-4	0.0	0.0	36.537740571872426	0.0
33	4.4898518124409303E-4	0.0	0.0	37.02937934533471	0.0
34	4.4898518124409303E-4	0.0	0.0	37.79624603489962	0.0
35	4.4898518124409303E-4	0.0	0.0	38.30247682675233	0.0
36	4.4898518124409303E-4	0.0	0.0	38.84170802942649	0.0
37	4.4898518124409303E-4	0.0	0.0	39.35489809158849	0.0
38	4.4898518124409303E-4	0.0	0.0	39.820046739357366	0.0
39	4.4898518124409303E-4	0.0	0.0	40.3783598122344	0.0
40	4.4898518124409303E-4	0.0	0.0	40.96989778852349	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTTAT	35	1.0176882E-7	46.000004	22
ATAAGCG	35	1.0176882E-7	46.000004	32
GCTACGA	70	0.0	46.000004	10
GACGAGG	35	1.0176882E-7	46.000004	1
AGTACGG	35	1.0176882E-7	46.000004	1
AAACTAG	30	1.858818E-6	46.0	9
TCGTTGA	25	3.413762E-5	46.0	23
AATCCCC	50	1.6370905E-11	46.0	36
GTACCCT	20	6.307271E-4	46.0	17
CTCAATC	25	3.413762E-5	46.0	26
GTACACG	20	6.307271E-4	46.0	24
TTAGCGG	60	0.0	46.0	1
CCCTACA	85	0.0	46.0	26
AGCCCGA	25	3.413762E-5	46.0	14
CTTTTCG	20	6.307271E-4	46.0	31
GACGTTG	20	6.307271E-4	46.0	38
GGCGTAC	20	6.307271E-4	46.0	7
ACTTAGC	20	6.307271E-4	46.0	15
GCGTTGG	45	3.092282E-10	46.0	1
ACGGGTG	40	5.5988494E-9	46.0	4
>>END_MODULE