FastQCFastQC Report
Thu 26 May 2016
SRR1544825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544825_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences339934
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT37481.1025669688821949No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT32400.9531261950849282Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT18380.5406931933845982No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTTG15940.46891455400165916Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT8150.2397524225290792No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC7430.21857184041608077No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC7180.21121747162684523No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC6540.19239028752640217No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA5850.17209222966811205No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT5750.16915048215241782No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC5730.168562132649279No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT4590.13502621097036482No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG4260.1253184441685739No Hit
GTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTTGA4120.12119999764660198Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1147281531120747No Hit
TCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC3780.11119805609324163No Hit
ATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC3600.10590291056499201No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC3510.10325533780086722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGG351.0167241E-746.0000041
GGTAAGG700.046.0000041
GGCACCG1100.046.0000047
ATAGCGG551.8189894E-1246.0000041
AATGGGC750.046.0000043
GCTCTGG551.8189894E-1246.0000041
CTACGAA351.0167241E-746.00000411
GTCACGG351.0167241E-746.0000041
GGTATGC551.8189894E-1246.0000047
GCATAAA206.305153E-446.08
TGTCAAC206.305153E-446.035
CTCACGT206.305153E-446.044
CCGGGAC206.305153E-446.04
GGTGTTC405.5915734E-946.08
CTCAATC253.412043E-546.026
GGTAAGT206.305153E-446.07
CGACGGT206.305153E-446.027
ACGCACA206.305153E-446.031
CTATAGC206.305153E-446.01
GCCGGTT206.305153E-446.029
Produced by FastQC (version 0.11.5)