FastQCFastQC Report
Thu 26 May 2016
SRR1544824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544824_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences969064
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT59560.6146136890855506Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT57970.5982061040344084Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT38360.3958458884036555Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.20163786911906748No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT16750.17284720101045958Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC15870.16376627343498468No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC15370.15860665549437394No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13930.14374695582541505No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC13810.14250864751966846No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT12240.12630744718615078No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11590.11959994386335682No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG11550.11918717442810794No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT11420.11784567376354915Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC11120.11474990299918271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG351.0193435E-746.0000041
CTTATCG351.0193435E-746.00000412
GTGTCGT351.0193435E-746.00000446
TATCGAC351.0193435E-746.00000414
ACGCGAG206.310963E-446.037
CGGCGAA206.310963E-446.011
CACGGTA206.310963E-446.03
CATGCGT301.861099E-646.013
TCGGGCG206.310963E-446.04
CGTCTAG206.310963E-446.019
CATTACG206.310963E-446.022
TCGCTCA253.4167562E-546.032
AACGAAG206.310963E-446.010
AATTCGG206.310963E-446.012
CGTAATG206.310963E-446.021
CATAAGT301.861099E-646.021
CGATGTT253.4167562E-546.016
GTCCAAC253.4167562E-546.08
GCGTCTA206.310963E-446.018
CGTTTAG206.310963E-446.029
Produced by FastQC (version 0.11.5)