FastQCFastQC Report
Thu 26 May 2016
SRR1544823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544823_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences703953
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT70461.0009190954509746Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT65240.9267664176443597Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT38660.5491843915715964Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTG29940.42531248535058447Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA19780.28098466801050637No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT15870.22544118712470862Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC15190.2157814513184829No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC14470.2055534957589498No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC13220.18779662846809375No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC11200.15910153092607035No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT10550.14986795993482518Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT9700.1377932901770431No Hit
CTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTGAAA9590.13623068585544773Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG9320.1323952025206228No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.12955410375408585No Hit
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC7670.10895613769669281No Hit
GTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTGA7540.10710942349844378Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7520.1068253136217901No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT7300.10370010497859942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA206.30978E-446.018
ATAGGCG206.30978E-446.033
TACGGGA1800.046.03
CTAACGG850.046.01
CTACGAT253.415798E-546.010
TGCGACC253.415798E-546.024
TCTCGAG253.415798E-546.012
GTCCGCA206.30978E-446.030
GTATCTA253.415798E-546.024
GCGATCT206.30978E-446.08
ACGTCCT253.415798E-546.022
ATTGGGT253.415798E-546.03
CGAGAGT206.30978E-446.015
CGGTCTA301.8603678E-646.030
ACGTAGG750.046.01
TAGGCGA206.30978E-446.034
ACGAGTT206.30978E-446.017
AGGGCGT206.30978E-446.05
TAAGCAA206.30978E-446.039
AATACGG405.6061253E-946.01
Produced by FastQC (version 0.11.5)