##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544823_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	703953
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83969100209815	34.0	33.0	34.0	31.0	34.0
2	33.18585473746116	34.0	33.0	34.0	31.0	34.0
3	33.27424132008813	34.0	34.0	34.0	31.0	34.0
4	36.53646905404196	37.0	37.0	37.0	35.0	37.0
5	36.53785551024003	37.0	37.0	37.0	35.0	37.0
6	36.468643503188424	37.0	37.0	37.0	35.0	37.0
7	36.30912575129305	37.0	37.0	37.0	35.0	37.0
8	36.363275673233865	37.0	37.0	37.0	35.0	37.0
9	38.21800887275145	39.0	39.0	39.0	37.0	39.0
10	38.10443168791098	39.0	39.0	39.0	35.0	39.0
11	37.52641582605657	39.0	35.0	39.0	35.0	39.0
12	37.479990851661974	39.0	35.0	39.0	35.0	39.0
13	37.47008536081244	39.0	35.0	39.0	35.0	39.0
14	38.76219719214209	41.0	36.0	41.0	35.0	41.0
15	38.822054881504876	41.0	36.0	41.0	35.0	41.0
16	38.82158467965901	41.0	36.0	41.0	35.0	41.0
17	38.877405167674546	41.0	36.0	41.0	35.0	41.0
18	38.861575985896785	41.0	36.0	41.0	35.0	41.0
19	38.81798216642304	41.0	36.0	41.0	35.0	41.0
20	38.73418821995219	41.0	35.0	41.0	35.0	41.0
21	38.69287154113982	41.0	35.0	41.0	35.0	41.0
22	38.66498047454873	41.0	35.0	41.0	35.0	41.0
23	38.65492014381642	41.0	35.0	41.0	35.0	41.0
24	38.58377903070233	41.0	35.0	41.0	35.0	41.0
25	38.51758711163956	40.0	35.0	41.0	35.0	41.0
26	38.47413108545599	40.0	35.0	41.0	35.0	41.0
27	38.4837325787375	40.0	36.0	41.0	35.0	41.0
28	38.51741948681233	40.0	36.0	41.0	35.0	41.0
29	38.569010999313875	40.0	36.0	41.0	35.0	41.0
30	38.50960788575374	40.0	35.0	41.0	35.0	41.0
31	38.462918689173854	40.0	35.0	41.0	35.0	41.0
32	38.41045637990036	40.0	35.0	41.0	35.0	41.0
33	38.26510576700433	40.0	35.0	41.0	35.0	41.0
34	38.2206113192216	40.0	35.0	41.0	35.0	41.0
35	38.16401663179218	40.0	35.0	41.0	35.0	41.0
36	38.041833758787874	40.0	35.0	41.0	35.0	41.0
37	38.046278657808124	40.0	35.0	41.0	35.0	41.0
38	37.964233407628065	40.0	35.0	41.0	35.0	41.0
39	37.88797831673421	40.0	35.0	41.0	35.0	41.0
40	37.834684986071515	40.0	35.0	41.0	35.0	41.0
41	37.86184162863146	40.0	35.0	41.0	35.0	41.0
42	37.815210674576285	40.0	35.0	41.0	35.0	41.0
43	37.72827873451779	40.0	35.0	41.0	35.0	41.0
44	37.61344862512128	40.0	35.0	41.0	35.0	41.0
45	37.61640762948663	40.0	35.0	41.0	35.0	41.0
46	37.55622179321631	39.0	35.0	41.0	35.0	41.0
47	37.46278231643306	39.0	35.0	41.0	35.0	41.0
48	37.38848616313873	39.0	35.0	41.0	34.0	41.0
49	37.3427941922259	39.0	35.0	41.0	34.0	41.0
50	37.23427274264049	39.0	35.0	41.0	34.0	41.0
51	37.123109071202194	39.0	35.0	41.0	34.0	41.0
52	36.68247454020368	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	7.0
14	4.0
15	4.0
16	16.0
17	35.0
18	54.0
19	104.0
20	162.0
21	274.0
22	416.0
23	692.0
24	1075.0
25	1881.0
26	2789.0
27	3105.0
28	3291.0
29	3607.0
30	4343.0
31	5428.0
32	7111.0
33	10419.0
34	31816.0
35	129079.0
36	24702.0
37	40056.0
38	76607.0
39	355144.0
40	1728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.692665561479245	21.350999285463658	23.609246640045573	22.347088513011524
2	27.671591711378458	23.93881409696386	25.669611465538182	22.7199827261195
3	27.36404276990083	24.031433916752963	26.14975715708293	22.454766156263272
4	26.587144312191295	25.55710395438332	24.354182736631564	23.50156899679382
5	24.036832004409387	33.57766782725552	21.657695897311328	20.727804271023775
6	81.99837205040677	3.1844455524729636	10.233779812004494	4.583402585115768
7	83.00781444215735	3.021508538212068	7.54837325787375	6.4223037617568215
8	70.20269819149858	5.803228340528416	14.679531161881545	9.31454230609146
9	46.72655702866527	19.80217429288603	16.712337329338748	16.758931349109954
10	38.71338001258607	20.0379854905086	27.654687173717562	13.593947323187768
11	36.85530141927089	17.09162401467143	25.874454686605496	20.178619879452178
12	20.41926094497786	30.281993258072625	29.413185255265624	19.88556054168389
13	14.099094683878043	33.05476359927438	31.353655712810376	21.4924860040372
14	13.641393672588936	24.529336475588572	40.71180888496817	21.11746096685432
15	15.735709628341665	18.279061244145563	44.22440134497616	21.76082778253662
16	15.521206671468125	23.74306239194946	28.25813655173002	32.477594384852395
17	18.546408638076688	23.56819276286911	32.11606456681057	25.76933403224363
18	22.09238400859148	28.50843735306192	28.110825580685074	21.288353057661517
19	25.862948236601024	22.673388706348295	26.094213676197132	25.369449380853553
20	19.532838129818327	30.623209219933717	27.323273002601027	22.520679647646933
21	19.626736444052373	24.2855702014197	26.95847592097768	29.129217433550252
22	18.4914333769442	28.550627669744998	23.84278495865491	29.115153994655895
23	19.248444143287973	22.039539571533894	36.10525134490513	22.606764940273003
24	20.6951316352086	21.75443531031191	31.27808248562049	26.272350568859
25	17.473751798770657	29.17240213480161	28.368087073994996	24.985758992432732
26	19.134231972873188	26.406592485577875	27.39884622979091	27.06032931175803
27	21.89506969925549	24.934335104758414	32.54606486512594	20.624530330860157
28	18.488876388054315	23.981004413646932	29.993621733269126	27.536497465029626
29	24.863023525718337	24.491123697178647	26.650216704808415	23.995636072294598
30	26.873100903043245	24.407737448380786	21.810262901074363	26.90889874750161
31	30.268498038931575	23.9670830296909	24.33912491316892	21.4252940182086
32	30.621078395858813	23.755421171583897	22.60548644582806	23.018013986729226
33	20.36258102458545	25.065451812834095	28.32873785607846	26.243229306502
34	25.85357261067145	20.2591650294835	29.562769105323795	24.324493254521254
35	30.5162418513736	22.293249691385647	25.23023554129324	21.960272915947513
36	25.532528450052773	26.11537986200783	27.47754466562398	20.874547022315408
37	25.3347879759018	26.02162360271211	27.545162816267567	21.098425605118525
38	24.415834579865418	24.40319169035433	26.50034874487359	24.680624984906665
39	29.97458637153333	20.40221435237864	28.51440366047165	21.108795615616383
40	20.2416922720693	19.30682872294031	32.16464735571835	28.286831649272038
41	23.517479149886427	22.773111273053743	24.922402489938957	28.787007087120877
42	23.336359103519698	18.958510014162876	29.862647080131772	27.842483802185658
43	25.135911062244215	23.308090170792653	28.517102704299862	23.038896062663277
44	21.722757059065025	25.25623159500705	26.719539514711922	26.301471831216006
45	23.210072263347126	23.979157699448685	26.577058411570093	26.233711625634097
46	26.3585779164234	19.54988472241755	28.990287703866592	25.101249657292463
47	22.622959203242264	17.806728574208787	34.19120310588917	25.379109116659777
48	20.904520614302374	17.548472696330577	30.685003118105897	30.86200357126115
49	22.31370560250471	20.632201297529807	32.39775950951271	24.65633359045277
50	21.00893099397261	21.46137597254362	30.34364510130648	27.186047932177292
51	21.52814179355724	18.26187259660801	30.562977926083136	29.647007683751614
52	22.203470970363078	17.44889218456346	36.04715087512945	24.300485969944017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	187.0
2	258.0
3	1130.0
4	2002.0
5	1376.5
6	751.0
7	833.0
8	915.0
9	956.5
10	998.0
11	948.5
12	899.0
13	828.0
14	758.0
15	759.0
16	770.0
17	781.0
18	814.0
19	847.0
20	889.0
21	931.0
22	971.5
23	1012.0
24	1333.5
25	1655.0
26	2089.5
27	2524.0
28	3167.0
29	3810.0
30	4749.0
31	5688.0
32	5727.0
33	5766.0
34	7396.0
35	9026.0
36	9953.0
37	10880.0
38	11840.5
39	14614.5
40	16428.0
41	22722.0
42	29016.0
43	36612.0
44	44208.0
45	48100.0
46	51992.0
47	56187.0
48	60382.0
49	75102.0
50	89822.0
51	93244.5
52	96667.0
53	84160.0
54	71653.0
55	59813.5
56	47974.0
57	40203.5
58	32433.0
59	29297.0
60	26161.0
61	25547.5
62	24934.0
63	22706.0
64	16053.0
65	11628.0
66	8915.0
67	6202.0
68	5133.0
69	4064.0
70	3349.5
71	2635.0
72	2099.5
73	1564.0
74	1315.5
75	1067.0
76	1016.5
77	966.0
78	651.5
79	337.0
80	221.0
81	105.0
82	75.5
83	46.0
84	26.0
85	6.0
86	5.0
87	4.0
88	3.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	703953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.831722272624617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.11190141435952	19.92615147311826
2	12.750803902730782	7.35255273752464
3	4.890057879604026	4.229663720454087
4	2.297154501347375	2.6492368240062807
5	1.3546121148486527	1.952790014122452
6	0.8166733486088505	1.4127659504726833
7	0.6171148608148882	1.2454738994126975
8	0.5129577039068636	1.183156324531672
9	0.41771221456182	1.083902630411644
>10	6.844703434904241	42.34071290329046
>50	0.2652784287168829	5.158491283949701
>100	0.10818617483895215	5.571107917897108
>500	0.007410011975270696	1.555893720953803
>1k	0.004446007185162418	2.4053399084097244
>5k	9.880015967027595E-4	1.9327606914448106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	7046	1.0009190954509746	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	6524	0.9267664176443597	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	3866	0.5491843915715964	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTG	2994	0.42531248535058447	Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1978	0.28098466801050637	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1587	0.22544118712470862	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1519	0.2157814513184829	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1447	0.2055534957589498	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1322	0.18779662846809375	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1120	0.15910153092607035	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1055	0.14986795993482518	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	970	0.1377932901770431	No Hit
CTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTGAAA	959	0.13623068585544773	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	932	0.1323952025206228	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.12955410375408585	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	767	0.10895613769669281	No Hit
GTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTGA	754	0.10710942349844378	Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	752	0.1068253136217901	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	730	0.10370010497859942	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9466541090101186	0.0
2	1.4205493832684853E-4	0.0	0.0	2.91098979619378	0.0
3	1.4205493832684853E-4	0.0	0.0	4.313498202294755	0.0
4	1.4205493832684853E-4	0.0	0.0	5.236997356357597	0.0
5	1.4205493832684853E-4	0.0	0.0	9.243088672113053	0.0
6	1.4205493832684853E-4	0.0	0.0	9.90989455261928	0.0
7	1.4205493832684853E-4	0.0	0.0	12.495294430167924	0.0
8	1.4205493832684853E-4	0.0	0.0	15.488534035652949	0.0
9	1.4205493832684853E-4	0.0	0.0	17.80900145322202	0.0
10	1.4205493832684853E-4	0.0	0.0	19.359815214936223	0.0
11	1.4205493832684853E-4	0.0	0.0	20.883922648244983	0.0
12	1.4205493832684853E-4	0.0	0.0	22.278902142614633	0.0
13	1.4205493832684853E-4	0.0	0.0	22.818852963194985	0.0
14	1.4205493832684853E-4	0.0	0.0	23.228539405329617	0.0
15	1.4205493832684853E-4	0.0	0.0	24.444245567530785	0.0
16	1.4205493832684853E-4	0.0	0.0	25.718762474199274	0.0
17	1.4205493832684853E-4	0.0	0.0	27.38890238410803	0.0
18	1.4205493832684853E-4	0.0	0.0	28.225037751099862	0.0
19	1.4205493832684853E-4	0.0	0.0	29.149815399607643	0.0
20	1.4205493832684853E-4	0.0	0.0	30.26608310498002	0.0
21	1.4205493832684853E-4	0.0	0.0	31.213873653496755	0.0
22	1.4205493832684853E-4	0.0	0.0	32.2738876032917	0.0
23	1.4205493832684853E-4	0.0	0.0	33.01484616160454	0.0
24	1.4205493832684853E-4	0.0	0.0	33.663753119881584	0.0
25	1.4205493832684853E-4	0.0	0.0	34.275299629378665	0.0
26	1.4205493832684853E-4	0.0	0.0	34.80843181291933	0.0
27	1.4205493832684853E-4	0.0	0.0	35.38432253289637	0.0
28	1.4205493832684853E-4	0.0	0.0	35.9305237707631	0.0
29	1.4205493832684853E-4	0.0	0.0	36.65628245067497	0.0
30	1.4205493832684853E-4	0.0	0.0	37.35760768119462	0.0
31	1.4205493832684853E-4	0.0	0.0	37.959068290070505	0.0
32	1.4205493832684853E-4	0.0	0.0	38.53851038350572	0.0
33	1.4205493832684853E-4	0.0	0.0	39.04195308493607	0.0
34	1.4205493832684853E-4	0.0	0.0	39.76330806175981	0.0
35	1.4205493832684853E-4	0.0	0.0	40.30482148666175	0.0
36	1.4205493832684853E-4	0.0	0.0	40.87687672330397	0.0
37	2.8410987665369706E-4	0.0	0.0	41.41895836795923	0.0
38	2.8410987665369706E-4	0.0	0.0	41.954931650266424	0.0
39	2.8410987665369706E-4	0.0	0.0	42.565199665318566	0.0
40	2.8410987665369706E-4	0.0	0.0	43.17575179024736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	6.30978E-4	46.0	18
ATAGGCG	20	6.30978E-4	46.0	33
TACGGGA	180	0.0	46.0	3
CTAACGG	85	0.0	46.0	1
CTACGAT	25	3.415798E-5	46.0	10
TGCGACC	25	3.415798E-5	46.0	24
TCTCGAG	25	3.415798E-5	46.0	12
GTCCGCA	20	6.30978E-4	46.0	30
GTATCTA	25	3.415798E-5	46.0	24
GCGATCT	20	6.30978E-4	46.0	8
ACGTCCT	25	3.415798E-5	46.0	22
ATTGGGT	25	3.415798E-5	46.0	3
CGAGAGT	20	6.30978E-4	46.0	15
CGGTCTA	30	1.8603678E-6	46.0	30
ACGTAGG	75	0.0	46.0	1
TAGGCGA	20	6.30978E-4	46.0	34
ACGAGTT	20	6.30978E-4	46.0	17
AGGGCGT	20	6.30978E-4	46.0	5
TAAGCAA	20	6.30978E-4	46.0	39
AATACGG	40	5.6061253E-9	46.0	1
>>END_MODULE